| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,760 | A→G | N431S (AAC→AGC) | igaA → | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,760 | 0 | A | G | 97.1% | 398.9 / 1.5 | 106 | N431S (AAC→AGC) | igaA | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (48/54); total (50/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC > NC_000913/3527715‑3527803 | ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 1:3833396/50‑1 (MQ=255)ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 1:7986195/1‑50 (MQ=255)ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 1:5021114/50‑1 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:3342933/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:6747645/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:2199519/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:1491639/1‑50 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc > 1:4548451/1‑50 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc > 1:8295400/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc < 1:156065/50‑1 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc < 1:309259/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc > 1:1153207/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:3470556/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:3938970/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:1849090/50‑1 (MQ=255) tCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg > 1:6944841/1‑50 (MQ=255) cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg < 1:2630572/49‑1 (MQ=255) cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc > 1:6679530/1‑50 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc < 1:454193/49‑1 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc < 1:3008137/49‑1 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:5415484/1‑50 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt < 1:4400450/50‑1 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCCGCTCATCTGGATCGCCGCCCtctc > 1:6450384/1‑50 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc < 1:2666122/50‑1 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc < 1:1364929/50‑1 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa > 1:596705/1‑50 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa < 1:4980094/50‑1 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa > 1:4976253/1‑50 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa < 1:4616056/50‑1 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:4729742/1‑50 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:1823753/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 1:481185/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 1:4394804/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt < 1:6269821/49‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt < 1:8252864/48‑1 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg < 1:4143100/49‑1 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 1:6955685/1‑50 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTg < 1:6972125/49‑1 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGc > 1:5959319/1‑50 (MQ=255) tttACCGTTTGACTGCTCACAGATCATCTGGAGCGACGCCCGCTCATTg < 1:3754609/49‑1 (MQ=39) ttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 1:7264497/1‑49 (MQ=255) ttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 1:5610671/1‑49 (MQ=255) ttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc < 1:6413690/49‑1 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGcc < 1:3935220/49‑1 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGcc < 1:6609223/49‑1 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa > 1:2038632/1‑50 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCCCTCATTGcc < 1:3131464/49‑1 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa < 1:1085228/49‑1 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAtt < 1:7453732/50‑1 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAtt < 1:5968581/50‑1 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa > 1:3293881/1‑50 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa > 1:3983499/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 1:869472/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAcc < 1:6498275/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAcc < 1:5289896/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:1112667/1‑49 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:5282390/1‑49 (MQ=255) ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:3258941/1‑48 (MQ=255) ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:699093/1‑48 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCa < 1:7164654/49‑1 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:547246/1‑49 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg < 1:6327168/49‑1 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:2931832/1‑49 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg < 1:2280518/49‑1 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:4869554/1‑49 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg < 1:497105/49‑1 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa < 1:3969641/50‑1 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 1:7997763/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGa < 1:6780086/49‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:1322474/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:2695968/50‑1 (MQ=255) gCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:3969937/1‑50 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt < 1:4164428/49‑1 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt < 1:6655735/49‑1 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg < 1:7774958/50‑1 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg < 1:1772969/50‑1 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:7208806/1‑49 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAGTCTGAAc > 1:1473775/1‑50 (MQ=39) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:2380358/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:3930147/1‑50 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt < 1:3625666/50‑1 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt < 1:5273765/50‑1 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt < 1:6994271/50‑1 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:1220014/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:5940508/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg > 1:4501563/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg > 1:7357459/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg > 1:2131627/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg > 1:2477070/1‑50 (MQ=255) atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt > 1:7469439/1‑50 (MQ=255) atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt > 1:5225062/1‑50 (MQ=255) atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt < 1:8064643/50‑1 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc < 1:1709385/50‑1 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc < 1:4294002/50‑1 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc < 1:2807297/50‑1 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc < 1:2891008/50‑1 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:5973339/1‑50 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:4199130/1‑50 (MQ=255) tcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:1131811/1‑49 (MQ=255) cTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaac > 1:3481762/1‑49 (MQ=255) | CCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC > NC_000913/3527715‑3527803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |