BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	420.1 / NA	105	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (41/64); total (41/64)

Only 100 of 105 total aligned reads displayed.

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:3675660/1‑50 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             <  1:5704231/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAGAACAGACGATTGTATGCCCggg                                            <  1:7578992/49‑1 (MQ=38)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:5644006/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:7331466/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           <  1:7250249/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:399828/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:2565256/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:5873883/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:5987320/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:1402098/50‑1 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         <  1:2096502/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  1:4373316/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        >  1:271403/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  1:7851405/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  1:1212734/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       >  1:7647531/1‑50 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:4612022/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       >  1:1832595/1‑50 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       >  1:353164/1‑50 (MQ=255)

       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      <  1:393985/50‑1 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:1036214/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:6307778/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:6025488/50‑1 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:8422853/50‑1 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:858064/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    <  1:716443/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    <  1:413855/50‑1 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:6897379/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:4286199/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                   <  1:4932404/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:3593400/49‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:4497993/50‑1 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                <  1:1838957/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:3601364/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:1483832/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:4600880/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:6252754/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  1:697949/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                >  1:3263706/1‑49 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                >  1:4742388/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGGTATTCTACAGaaaa                             <  1:5751627/49‑1 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  1:571298/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             <  1:490815/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           <  1:7259320/50‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:6425132/1‑50 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:721235/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:1903012/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:1873785/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:3751688/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:5625077/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:3229990/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:7621261/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:788261/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:5263481/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:5080949/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:3231283/50‑1 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:2813474/1‑50 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:4593119/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      >  1:5796982/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      <  1:1903813/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:7760781/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:5439525/50‑1 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:6260930/1‑49 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                    <  1:5534024/50‑1 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:6629089/1‑49 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:1954529/1‑49 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:4337264/1‑49 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                  <  1:5872093/50‑1 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 <  1:6282572/50‑1 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 <  1:5016947/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:821767/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:892047/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:6543672/50‑1 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              <  1:113935/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:1089504/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:6546785/1‑50 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:3350513/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:4706190/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:5708433/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:6525942/50‑1 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:1608351/1‑49 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:7606874/50‑1 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:2674746/50‑1 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          <  1:4863680/50‑1 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa           >  1:7046061/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:637035/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:6786530/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:6542143/1‑49 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa        <  1:6473704/50‑1 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa        <  1:662968/50‑1 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:2723063/1‑48 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:6960041/1‑50 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:8501276/1‑50 (MQ=255)

                                       tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:1166820/1‑49 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:8468895/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:317785/1‑50 (MQ=255)

                                          aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:6469081/1‑49 (MQ=255)

                                            gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  1:8402176/1‑49 (MQ=255)

                                            gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  1:5909580/1‑49 (MQ=255)

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`