Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,363	Δ2 bp	pseudogene (915‑916/1358 nt)	gatC ←	galactitol‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,361	0	C	.	100.0%	34.0 / NA	8	pseudogene (918/1358 nt)	gatC	galactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (4/4);  total (4/4)
*	NC_000913	2,173,362	0	C	.	100.0%	34.0 / NA	8	pseudogene (917/1358 nt)	gatC	galactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (4/4);  total (4/4)

ACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCA  >  NC_000913/2173312‑2173404                                                  ||                                           aCAGCAATTAAAATGGTGAGTGGGATAAACATCAGGCTTGCCGATaccac                                             >  1:3927529/1‑50 (MQ=255)              tGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTcc                               >  2:3732529/1‑49 (MQ=255)                   aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag                         <  1:1556271/50‑1 (MQ=255)                      gggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCaa                      >  1:3210856/1‑50 (MQ=255)                          tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc                  <  1:1842333/50‑1 (MQ=255)                          tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc                  <  2:3435670/50‑1 (MQ=255)                               aTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt              >  1:5090649/1‑49 (MQ=255)                                        gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt    >  1:5122236/1‑50 (MQ=255)                                          cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa  <  1:2083664/50‑1 (MQ=255)                                                  ||                                           ACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCA  >  NC_000913/2173312‑2173404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑