| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 74.5% | 297.0 / 106.4 | 102 | coding (65/1386 nt) | yifK | putative transporter YifK |
| Reads supporting (aligned to +/- strand): ref base . (15/11); new base G (34/42); total (49/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 126 total aligned reads displayed.
CCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCT > NC_000913/3980902‑3980999 | ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑t < 1:311617/50‑1 (MQ=255)ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑t < 2:2054659/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑g > 1:4321034/1‑50 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑g < 1:3717044/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑g < 2:4867874/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑g < 1:1337746/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑g < 1:1136976/50‑1 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑gg > 1:3829941/1‑50 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑gg < 2:50756/50‑1 (MQ=255) gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑ggg < 1:2913417/50‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑gggg > 2:3647412/1‑50 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑gggg > 2:2249762/1‑50 (MQ=255) cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑gggggg < 1:2328840/50‑1 (MQ=255) cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑gggggg > 1:663257/1‑50 (MQ=255) cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑gggggg > 1:2761664/1‑50 (MQ=255) tACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCTCCC‑T‑‑ggggggg > 1:4086086/1‑49 (MQ=255) tACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑ggggggg > 1:1254296/1‑49 (MQ=255) aCAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑ggggggg > 2:3293980/1‑48 (MQ=255) aCAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑ggggggg > 2:2761018/1‑48 (MQ=255) aCAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCa > 2:2634010/1‑50 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGc < 2:3296667/48‑1 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCa < 1:4456399/49‑1 (MQ=255) aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCAc > 1:4094364/1‑49 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCa > 2:2894814/1‑50 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCa < 2:4537508/50‑1 (MQ=255) gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCAt > 2:1460649/1‑50 (MQ=255) gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCAt > 2:1177230/1‑50 (MQ=255) gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCAtt < 1:1361571/50‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTg < 2:952927/50‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTg > 1:4094963/1‑50 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTg < 2:5090561/50‑1 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTgg < 1:4652474/50‑1 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTgg > 1:5269781/1‑50 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTg < 1:2606120/49‑1 (MQ=39) ggCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTgg < 2:4654046/49‑1 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGc > 2:3835420/1‑50 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGc < 1:526763/50‑1 (MQ=255) cTGGAAGCTCGACATATCGAACTCATTGCCC‑TG‑GGGGGGCACCATTGGCg > 1:4460627/1‑50 (MQ=255) cTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCg > 2:1266857/1‑50 (MQ=255) cTGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCg < 2:106456/50‑1 (MQ=255) tGGAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGt > 2:556172/1‑50 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTc > 2:616946/1‑50 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCg > 1:398228/1‑50 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCg > 2:5279974/1‑50 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTc < 2:2194685/49‑1 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCg > 1:2142731/1‑50 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCg < 2:4292699/50‑1 (MQ=255) aGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGc > 2:5232799/1‑50 (MQ=255) aGCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGc < 1:3198310/50‑1 (MQ=255) gCTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGcc > 2:1674479/1‑50 (MQ=255) cTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCt < 2:3469713/50‑1 (MQ=255) cTCGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCt < 1:4829/50‑1 (MQ=255) cGACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGt > 1:1162022/1‑50 (MQ=255) gACATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGtt < 2:1719424/50‑1 (MQ=255) gACATATCGAACTCAGCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGt < 2:3106090/49‑1 (MQ=38) gACAGATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGtt < 1:3464357/50‑1 (MQ=255) aCATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGttt < 2:4091208/50‑1 (MQ=255) aCATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTa > 1:2081253/1‑50 (MQ=255) cATATCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTa < 1:2529128/50‑1 (MQ=255) cATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTAt < 1:4993940/50‑1 (MQ=255) atatCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTAt > 2:2188048/1‑50 (MQ=255) atatCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTAt > 1:4054246/1‑50 (MQ=255) atatCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTAt < 1:5196079/50‑1 (MQ=255) tatCGAACTCATCGCCC‑TGGGGGGGGCACCATTGGCGTCGGCCTGTTTAt < 1:3142322/50‑1 (MQ=255) tatCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg > 2:4544842/1‑50 (MQ=255) atCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg > 2:3968971/1‑50 (MQ=255) atCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg > 1:320703/1‑50 (MQ=255) tCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggg > 2:531976/1‑50 (MQ=255) tCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggg < 1:2537937/50‑1 (MQ=255) tCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg < 1:2875542/49‑1 (MQ=39) tCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg < 2:2483979/49‑1 (MQ=39) tCGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg < 1:1034526/49‑1 (MQ=39) cGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg > 2:3414332/1‑50 (MQ=255) cGAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg > 2:1292261/1‑50 (MQ=255) gAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggggg < 1:493072/50‑1 (MQ=255) gAACTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggggg > 2:426718/1‑50 (MQ=255) gAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATggggg < 2:4846369/49‑1 (MQ=255) aaCTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc < 2:3998801/50‑1 (MQ=255) aaCTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc < 2:5149049/50‑1 (MQ=255) aaCTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc > 1:3147026/1‑50 (MQ=255) aaCTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgcc < 2:2866802/50‑1 (MQ=255) aCTCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgcc < 1:834293/50‑1 (MQ=255) aCTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg > 1:3285662/1‑50 (MQ=255) tCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 2:503127/1‑50 (MQ=255) tCATCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 2:1657003/1‑50 (MQ=255) cATCGCCCTT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 1:1031973/1‑49 (MQ=39) aTCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc < 2:2593793/49‑1 (MQ=39) aTCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc < 2:4085751/49‑1 (MQ=39) aTCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc < 2:911544/49‑1 (MQ=39) aTCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc < 1:226453/49‑1 (MQ=39) aTCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 1:2547512/48‑1 (MQ=39) aTCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:4695083/50‑1 (MQ=255) tCGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg < 1:4297356/50‑1 (MQ=255) tCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg > 1:376778/1‑49 (MQ=255) cGCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg > 2:2557885/1‑49 (MQ=39) gCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt < 1:1792824/49‑1 (MQ=39) gCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt < 1:55935/49‑1 (MQ=39) gCCC‑TG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa < 2:378187/50‑1 (MQ=255) gCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc > 1:3533892/1‑50 (MQ=255) t‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCt > 2:2111687/1‑49 (MQ=255) | CCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑T‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCT > NC_000913/3980902‑3980999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |