| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,944,197 | 0 | T | C | 30.8% | 250.6 / 146.4 | 156 | *262Q (TAA→CAA) | znuB | Zn(2(+)) ABC transporter membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (54/54); new base C (31/17); total (85/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 160 total aligned reads displayed.
TGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCC > NC_000913/1944149‑1944245 | tgtgCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCt < 2:5321559/49‑1 (MQ=255)tgtgCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCt > 2:2255777/1‑49 (MQ=255)tgtgCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCt > 1:4415827/1‑49 (MQ=255) tgCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCaa < 1:2945643/49‑1 (MQ=255) cGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATc < 1:3277854/49‑1 (MQ=255) cGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATc > 1:2256240/1‑49 (MQ=255) cGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATc < 2:2509291/49‑1 (MQ=255) cGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCt < 2:1829374/50‑1 (MQ=255) ggCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTa < 2:2715823/50‑1 (MQ=255) gCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTAt > 1:1369659/1‑50 (MQ=255) cACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATc < 1:2370535/50‑1 (MQ=255) cACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATc > 1:2606156/1‑50 (MQ=255) cACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATc < 1:4631028/50‑1 (MQ=255) cACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATc < 1:1101383/50‑1 (MQ=255) cACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATc > 1:3138520/1‑50 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCg > 1:5346376/1‑50 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCg < 1:3734548/50‑1 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCg < 2:4248530/50‑1 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCg > 2:4387701/1‑50 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCg > 2:3241339/1‑50 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCg < 1:330765/50‑1 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCg > 2:3964792/1‑50 (MQ=255) aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCg > 2:1029482/1‑50 (MQ=255) cTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGc > 1:4923530/1‑50 (MQ=255) cTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGc > 2:284068/1‑50 (MQ=255) cTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGc < 2:2540420/50‑1 (MQ=255) cTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGc > 1:4350700/1‑50 (MQ=255) cTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGc > 2:2137192/1‑50 (MQ=255) cTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGc > 2:3463706/1‑50 (MQ=255) tGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCt > 2:1785588/1‑50 (MQ=255) tGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCt > 1:204074/1‑50 (MQ=255) tGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCt > 1:4463417/1‑50 (MQ=255) ttatttatTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTg > 1:3438647/1‑49 (MQ=255) ttatttatTCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGa < 1:3951713/50‑1 (MQ=255) ttatttatTCTCAGAATGATGAAAAAGCAGGCCAGCCAATCTATCGCTg > 1:63114/1‑49 (MQ=38) tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAaca > 2:5023504/1‑50 (MQ=255) tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAaca < 2:3372818/50‑1 (MQ=255) tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAac < 1:2697942/49‑1 (MQ=255) tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAac < 1:4223564/49‑1 (MQ=255) tttattCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAaca < 2:3890895/50‑1 (MQ=255) tttattCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAaca > 1:78983/1‑50 (MQ=255) tattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAacac < 1:241598/49‑1 (MQ=255) tattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAAcaca > 2:5346507/1‑50 (MQ=255) attCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACAt > 1:2029186/1‑50 (MQ=255) attCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACAt > 2:4709983/1‑50 (MQ=255) attCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACAt > 2:3260312/1‑50 (MQ=255) attCTCAGTATGAGGAAAAAGCAGGCCAGCTAATCTATCGCTGAAcaca < 1:3578385/49‑1 (MQ=255) ttCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACGCAtt < 2:3915244/50‑1 (MQ=255) ttCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACAtt < 1:694756/50‑1 (MQ=255) ttCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACAtt > 2:703954/1‑50 (MQ=255) ttCTCAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACAtt > 2:3970670/1‑50 (MQ=255) tctcAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACAttt > 2:4897565/1‑50 (MQ=255) ctcAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACAttt < 2:739771/49‑1 (MQ=255) ctcAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTg > 2:2849576/1‑50 (MQ=255) ctcAGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTg > 1:3481318/1‑50 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTg < 2:4594308/47‑1 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCg > 1:4151067/1‑50 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCg > 2:3049638/1‑50 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTg < 1:1266641/47‑1 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTc < 2:360762/49‑1 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCg < 1:969990/50‑1 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCg < 1:942598/50‑1 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCg > 2:2810784/1‑50 (MQ=255) aGTATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCg < 2:2966027/50‑1 (MQ=255) tATGATGAAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCGGa > 1:2191609/1‑50 (MQ=255) tgatgaAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCGGATg < 2:594255/50‑1 (MQ=255) atgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcg > 1:4446220/1‑50 (MQ=255) atgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcg > 1:3743575/1‑50 (MQ=255) tgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcgg < 2:528195/50‑1 (MQ=255) tgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcgg < 1:3191100/50‑1 (MQ=255) tgaAAAAGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCGGATgcgg > 1:526453/1‑50 (MQ=255) aaaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcggcg > 2:1360974/1‑50 (MQ=255) aaaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcggcg < 2:370358/50‑1 (MQ=255) aaaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcggc < 2:1668727/49‑1 (MQ=255) aaaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATgcggc < 1:1256016/49‑1 (MQ=255) aaaaaGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCGGATgcggcg > 1:4972937/1‑50 (MQ=255) aaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGgcgc < 1:5175791/50‑1 (MQ=255) aaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGgcgc > 2:3597760/1‑50 (MQ=255) aaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGgcgc > 2:1274559/1‑49 (MQ=255) aaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGgcgc > 2:3107238/1‑48 (MQ=255) aaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGgcgc > 2:2662681/1‑48 (MQ=255) aaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGa < 1:2334446/50‑1 (MQ=255) aaGCAGGCCAGCTAATCTATCGCTGAACACATTTGCCGGATGCGgcgc > 1:5121513/1‑48 (MQ=255) aaGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCGGATGCGgcgc > 2:1614476/1‑48 (MQ=255) aaGCAGGCCAGCCAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGa < 2:2227960/50‑1 (MQ=255) gCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAgc < 2:4531931/50‑1 (MQ=255) gCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAgc < 1:337003/50‑1 (MQ=255) aGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAgcgc > 2:3433170/1‑50 (MQ=255) aGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAgcgc < 2:4951015/50‑1 (MQ=255) aGGCCAGCCAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAgcgc > 1:1965250/1‑50 (MQ=255) ggCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGcc < 1:404053/50‑1 (MQ=255) ggCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGcc > 1:2717192/1‑50 (MQ=255) gccagccaATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCt > 1:3894811/1‑50 (MQ=255) cAGCCAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTa > 1:4365270/1‑50 (MQ=255) aGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTAt > 2:4431838/1‑50 (MQ=255) aGCCAATCTATCGCTGAACACATTTGCCGGATGCGGCGCGAGCGCCTTAt > 2:1749795/1‑50 (MQ=25) gCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATc < 2:201440/50‑1 (MQ=255) cTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATcc < 2:4329088/50‑1 (MQ=255) cTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATcc < 2:2082909/50‑1 (MQ=255) cTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATcc < 1:1111163/50‑1 (MQ=255) | TGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCC > NC_000913/1944149‑1944245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |