| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,915,727 | 0 | G | A | 32.8% | 158.3 / 128.9 | 122 | R224Q (CGG→CAG) | barA | sensory histidine kinase BarA |
| Reads supporting (aligned to +/- strand): ref base G (39/43); new base A (18/22); total (57/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 129 total aligned reads displayed.
AACATGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATC > NC_000913/2915678‑2915776 | aaCATGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCg > 1:4778580/1‑50 (MQ=255)aaCATGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCg > 1:2175792/1‑50 (MQ=255)aaCATGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCg < 2:3683790/50‑1 (MQ=255) cATGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCggg > 2:1358280/1‑50 (MQ=255) cATGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCagg < 1:4931185/50‑1 (MQ=255) aTGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGt > 1:4975449/1‑50 (MQ=255) aTGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGt < 1:2589749/50‑1 (MQ=255) aTGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGt < 2:2551716/50‑1 (MQ=255) aTGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGt > 1:180050/1‑50 (MQ=255) tGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTg > 2:3889836/1‑50 (MQ=255) tGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTg > 1:353300/1‑50 (MQ=255) gTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGa < 2:3949952/50‑1 (MQ=255) gTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTgg < 1:5096818/49‑1 (MQ=255) tGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGaa < 1:427382/50‑1 (MQ=255) tGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTgg > 1:1011815/1‑48 (MQ=255) gAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAg > 1:4056409/1‑50 (MQ=255) aaTACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAgg < 1:27923/50‑1 (MQ=255) aaTACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAg < 1:844712/49‑1 (MQ=255) gtACCGTCGACCGCAGGCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGa < 2:1586893/49‑1 (MQ=255) aTACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGa < 1:3351793/50‑1 (MQ=255) aTACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGa > 2:1939108/1‑50 (MQ=255) tACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGAt > 1:1154748/1‑50 (MQ=255) tACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGAt < 1:1650450/50‑1 (MQ=255) tACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGAt > 2:569863/1‑50 (MQ=255) aCCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGAtt > 1:2109367/1‑50 (MQ=255) ccGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGAtt < 1:4532465/49‑1 (MQ=255) cGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTa > 1:4167739/1‑50 (MQ=255) gTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTag < 2:3469357/50‑2 (MQ=255) gTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTAt > 1:3618017/1‑50 (MQ=255) gTCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTAt < 2:2526604/50‑1 (MQ=255) tCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATg > 1:4906414/1‑50 (MQ=255) tCGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATg < 1:718878/50‑1 (MQ=255) cGACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGc > 1:4159190/1‑50 (MQ=255) gACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCt > 2:1666174/1‑50 (MQ=255) gACCGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCt > 2:1549948/1‑50 (MQ=255) aCCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTc < 2:3610997/50‑1 (MQ=255) aCCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTc > 2:152189/1‑50 (MQ=255) aCCGCATCCGTCGCGGGCAACTCGAAAGCCGGGTGGAAGGAGTTATGCTc < 2:3738659/50‑1 (MQ=255) ccGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCg < 1:39241/50‑1 (MQ=255) ccGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCg < 2:2875654/50‑1 (MQ=255) ccGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCg > 2:410520/1‑50 (MQ=255) ccGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCg < 2:4409429/50‑1 (MQ=255) ccGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCg < 1:873649/50‑1 (MQ=255) ccGCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCg < 2:1706199/50‑1 (MQ=255) gCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGc < 2:606510/50‑1 (MQ=255) gCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGc < 2:4819773/50‑1 (MQ=255) gCATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGc < 2:2861143/50‑1 (MQ=255) cATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCg < 2:1753960/50‑1 (MQ=255) cATCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCg < 2:266233/50‑1 (MQ=255) aTCCGTCGCGGGCAACTCGACAGCCGGGCGGAAGGATTTATGCTCGGc > 2:250996/1‑48 (MQ=255) tCCGTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAg > 1:681451/1‑50 (MQ=255) cGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGc < 2:746498/49‑1 (MQ=255) cGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGc < 1:4370922/49‑1 (MQ=255) cGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCt < 2:3939052/50‑1 (MQ=255) cGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCt > 2:4044931/1‑50 (MQ=255) cGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCt > 1:340120/1‑50 (MQ=255) gTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTg > 2:642499/1‑50 (MQ=255) gTCGCGGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTg > 2:55421/1‑50 (MQ=255) tCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTg > 2:187685/1‑49 (MQ=255) cgcgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGa > 2:4207096/1‑50 (MQ=255) cgcgGGCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGa > 2:4208227/1‑50 (MQ=255) gcgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGat < 1:3856079/50‑1 (MQ=255) gcgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGa < 1:2252058/49‑1 (MQ=255) gcgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGa > 1:1915177/1‑49 (MQ=255) gcgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGa < 1:3242892/49‑1 (MQ=255) cgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGata > 2:1922574/1‑50 (MQ=255) cgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGata < 1:3493830/50‑1 (MQ=255) cgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGata < 2:3618111/50‑1 (MQ=255) cgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGata < 2:4928791/50‑1 (MQ=255) gggCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGatat > 2:529790/1‑50 (MQ=255) gggCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGatat < 2:3879002/50‑1 (MQ=255) gggCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGatat < 2:4071508/50‑1 (MQ=255) gggCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGatat < 2:884829/50‑1 (MQ=255) ggCAACTCGACAGCCGGGTGTAAGGATTTATGCTCGGCGAGCTGGATATg < 1:2788967/50‑1 (MQ=255) ggCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATg < 2:2649346/50‑1 (MQ=255) ggCAACTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGATATg < 2:631646/50‑1 (MQ=255) gCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGc < 1:5033002/50‑1 (MQ=255) cAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCt < 1:4945646/50‑1 (MQ=255) aaCTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCt > 2:3845301/1‑49 (MQ=255) cTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGa > 2:5219655/1‑49 (MQ=255) cTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGa > 1:5184058/1‑49 (MQ=255) cTCGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGa > 2:3657473/1‑49 (MQ=255) tCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGaa > 2:3829258/1‑49 (MQ=255) tCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGaa > 1:4045920/1‑49 (MQ=255) tCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGaa > 1:126144/1‑49 (MQ=255) cGACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGaaa > 1:1777126/1‑49 (MQ=255) gACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGaaaaa < 1:2388681/50‑1 (MQ=255) gACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGaaaa < 1:3945273/49‑1 (MQ=255) gACAGCCAGGTGGAAGGATTTATGCTCGGCGGGCTGGATATGCTGaaa < 2:4177283/48‑1 (MQ=39) gACAGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGaaaa < 2:899244/49‑1 (MQ=255) aCAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAAc < 1:1409399/50‑1 (MQ=255) aCAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAAc < 1:2364984/50‑1 (MQ=255) aCAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAAc < 1:2881373/50‑1 (MQ=255) cAGCCGGGTGGACGGATTTATGCTCGGCGAGCTGGATATGCTGAACAACg > 1:215045/1‑50 (MQ=255) aGCCAGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACgg > 1:4799027/1‑50 (MQ=255) aGCAGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACgg > 2:2329316/1‑50 (MQ=255) gCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAGCGGt < 2:3425594/50‑1 (MQ=255) ccGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTa > 1:4670590/1‑50 (MQ=255) cGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTAt < 2:4788407/50‑1 (MQ=255) gggTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATc < 1:3521790/50‑1 (MQ=255) | AACATGGTGAATACCGTCGACCGCATCCGTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATC > NC_000913/2915678‑2915776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |