| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,270,811 | C→T | V347M (GTG→ATG) | chaA ← | Na(+)/K(+):H(+) antiporter ChaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,270,811 | 0 | C | T | 96.8% | 471.5 / 3.1 | 123 | V347M (GTG→ATG) | chaA | Na(+)/K(+):H(+) antiporter ChaA |
| Reads supporting (aligned to +/- strand): ref base C (0/4); new base T (51/68); total (51/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG > NC_000913/1270764‑1270857 | catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt > 1:2298283/1‑50 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt < 2:875515/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt < 1:4908838/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt < 1:2789992/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGtt < 2:4522181/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt < 2:1253415/49‑1 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt > 1:3233530/1‑48 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa < 2:3786603/50‑1 (MQ=255) caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:1366763/1‑50 (MQ=255) caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:2971453/1‑50 (MQ=255) caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 2:439915/1‑50 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt < 2:4999137/50‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt < 2:2119067/50‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt < 2:3972234/50‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt < 2:1927392/50‑1 (MQ=255) aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa < 1:4732544/50‑1 (MQ=255) aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc < 2:3501848/50‑1 (MQ=255) aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc < 1:2747967/50‑1 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg > 2:4881509/1‑50 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg < 1:3270016/50‑1 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc > 2:5073264/1‑50 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc < 1:4266696/50‑1 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc > 1:4053676/1‑50 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc < 1:2982714/50‑1 (MQ=255) gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc < 2:4966647/50‑1 (MQ=255) gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc < 2:3113359/50‑1 (MQ=255) gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc < 1:4933719/50‑1 (MQ=255) gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc < 1:4857585/50‑1 (MQ=255) cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg > 2:2566174/1‑50 (MQ=255) cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg > 1:4840055/1‑50 (MQ=255) ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg > 1:3135437/1‑50 (MQ=255) ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg > 1:580132/1‑50 (MQ=255) ggcAAACAGTGCCAGATGCGCTGCGCCATGGAGCATGTTAGTACGTCCgg < 1:2899849/50‑1 (MQ=39) gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt < 1:1954664/50‑1 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg < 2:2207878/50‑1 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 2:1717299/1‑49 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:1809907/1‑49 (MQ=255) aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:1514048/1‑48 (MQ=255) aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 2:4811229/1‑48 (MQ=255) aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga < 1:1026257/50‑1 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 2:2852916/1‑47 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag < 1:454344/50‑1 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag < 2:1980780/50‑1 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag < 1:1121914/50‑1 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag < 2:5071145/50‑1 (MQ=255) cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga < 1:1722211/50‑1 (MQ=255) cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga < 1:339126/50‑1 (MQ=255) cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag > 1:4341522/1‑49 (MQ=255) cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag > 2:1635647/1‑49 (MQ=255) aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa < 2:1539573/50‑1 (MQ=255) aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa < 1:3495129/50‑1 (MQ=255) gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:5271458/1‑50 (MQ=255) gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg < 1:291203/50‑1 (MQ=255) tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:5336331/1‑49 (MQ=255) tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 2:2203949/1‑49 (MQ=255) tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 2:366212/1‑49 (MQ=255) gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:1110527/1‑48 (MQ=255) ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag < 1:821349/50‑1 (MQ=255) ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag < 1:3660692/50‑1 (MQ=255) ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga < 1:2203282/49‑1 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 2:3274016/1‑46 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga < 2:2887154/50‑1 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga > 2:830945/1‑49 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga > 1:325076/1‑49 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga > 1:3081267/1‑49 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga > 1:1066647/1‑49 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat < 2:3216334/50‑1 (MQ=255) gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata < 2:3940335/50‑1 (MQ=255) aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat > 1:4450641/1‑50 (MQ=255) tgcgctgcgcCATTGAGCATGTTAGTCCGTCCGGTGGAGAAGGAGATATg < 2:3360415/50‑1 (MQ=39) tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg < 1:138511/50‑1 (MQ=255) tgcgctgcgcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATg < 2:2790038/50‑1 (MQ=255) cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg > 2:3857011/1‑49 (MQ=255) cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg > 1:1055685/1‑49 (MQ=255) cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc < 1:1346805/50‑1 (MQ=255) cgctgcgcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATgg < 2:1341795/49‑1 (MQ=255) gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca < 1:3636161/50‑1 (MQ=255) gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:641424/1‑50 (MQ=255) gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:476902/1‑50 (MQ=255) ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 2:1429169/1‑49 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:3473932/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 2:601624/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:1914829/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:2308522/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 2:3901218/1‑49 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg < 2:1975291/49‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 1:1836792/1‑49 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 1:935529/1‑49 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 2:1189601/1‑49 (MQ=255) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa > 1:5384659/1‑50 (MQ=255) ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc < 1:2691442/50‑1 (MQ=255) ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc > 1:4235650/1‑50 (MQ=255) cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt < 2:4826366/50‑1 (MQ=255) cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt < 1:4095781/50‑1 (MQ=255) aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt > 1:2595171/1‑49 (MQ=255) aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa < 2:3349612/50‑1 (MQ=255) ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa < 2:685031/50‑1 (MQ=255) aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga < 2:3161139/50‑1 (MQ=255) gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag < 1:5158100/50‑1 (MQ=255) gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag < 2:3608041/50‑1 (MQ=255) | CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG > NC_000913/1270764‑1270857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |