| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 119,028 | T→C | V99A (GTC→GCC) | ampD → | 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,028 | 0 | T | C | 93.9% | 461.4 / 12.7 | 132 | V99A (GTC→GCC) | ampD | 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base C (65/59); total (68/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 146 total aligned reads displayed.
AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGGAT > NC_000913/118981‑119076 | aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGtctc < 1:2225/50‑1 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc < 1:4961844/50‑1 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc < 2:2134644/50‑1 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc > 2:1781852/1‑50 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc > 2:1578651/1‑50 (MQ=255) aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc > 1:2349046/1‑50 (MQ=255) tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct < 2:679314/50‑1 (MQ=255) tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct < 1:3930493/50‑1 (MQ=255) tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct < 1:935981/50‑1 (MQ=255) gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCa < 2:1969490/50‑1 (MQ=255) gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCa < 2:3966020/50‑1 (MQ=255) gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCa < 2:112005/50‑1 (MQ=255) tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg > 2:3248078/1‑50 (MQ=255) tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg > 2:3604089/1‑50 (MQ=255) tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg > 1:4903378/1‑50 (MQ=255) tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg > 2:321633/1‑50 (MQ=255) tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg > 2:2140887/1‑50 (MQ=255) tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTAAg < 2:4015754/50‑1 (MQ=39) ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg > 2:1774878/1‑49 (MQ=255) ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg > 1:306055/1‑49 (MQ=255) cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGt < 2:4099269/49‑1 (MQ=255) cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa < 1:4725052/50‑1 (MQ=255) cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa > 1:68303/1‑50 (MQ=255) aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt > 1:3240325/1‑50 (MQ=255) aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt < 1:1752788/50‑1 (MQ=255) aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt > 2:5057666/1‑50 (MQ=255) gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt > 1:1224074/1‑49 (MQ=255) gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc < 1:4907314/50‑1 (MQ=255) tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt > 2:2754615/1‑48 (MQ=255) tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg < 2:3331317/50‑1 (MQ=255) tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg < 1:68719/50‑1 (MQ=255) tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg < 1:3730459/49‑1 (MQ=255) gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg > 2:4717739/1‑50 (MQ=255) ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg > 1:2191419/1‑50 (MQ=255) ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg < 2:1158255/50‑1 (MQ=255) tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc > 2:5025889/1‑50 (MQ=255) ccTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGgc < 2:3619754/49‑1 (MQ=255) ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg > 1:245872/1‑50 (MQ=255) ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc < 2:2396714/49‑1 (MQ=255) cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc < 1:3356879/50‑1 (MQ=255) cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc > 1:1772660/1‑50 (MQ=255) cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc < 2:4295277/50‑1 (MQ=255) tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc > 2:3815184/1‑49 (MQ=255) tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc > 1:5251329/1‑49 (MQ=255) ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg > 1:2254949/1‑49 (MQ=255) ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg > 1:1958871/1‑49 (MQ=255) tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa > 2:733918/1‑50 (MQ=255) tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGa < 1:2275590/49‑1 (MQ=255) tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGa < 1:22162/49‑1 (MQ=255) tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGa < 2:4859217/49‑1 (MQ=255) cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc > 2:4979550/1‑50 (MQ=255) cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc > 2:2827042/1‑50 (MQ=255) cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc > 1:862238/1‑50 (MQ=255) cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc > 2:5350511/1‑50 (MQ=255) gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc < 2:309759/49‑1 (MQ=255) gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc < 2:2081418/49‑1 (MQ=255) gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc < 2:802175/49‑1 (MQ=255) aTAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGc > 1:1330045/1‑50 (MQ=255) aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc > 1:2925359/1‑50 (MQ=255) tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt > 2:5026213/1‑50 (MQ=255) tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt > 2:915377/1‑50 (MQ=255) tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt < 1:5231140/50‑1 (MQ=255) tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt < 2:1801622/50‑1 (MQ=255) tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt > 1:5350171/1‑50 (MQ=255) aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg > 2:4449684/1‑50 (MQ=255) aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg > 2:4798730/1‑50 (MQ=255) aaCGTGCATGGTATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg > 2:4969102/1‑49 (MQ=38) aaCGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTg < 2:294122/49‑1 (MQ=255) aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg < 1:5037994/49‑1 (MQ=255) aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg > 2:534987/1‑49 (MQ=255) aCGTGCATGGCATGCTGGAGCCTCTCAGTATCAGGGGCACGAACGCTGCa < 2:1357217/50‑1 (MQ=255) aCGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCa < 1:3392776/50‑1 (MQ=255) cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa < 1:2789574/50‑1 (MQ=255) gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt > 2:927724/1‑50 (MQ=255) gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt < 1:317555/50‑1 (MQ=255) gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt > 1:1436495/1‑50 (MQ=255) tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt > 1:2900417/1‑49 (MQ=255) tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt > 1:13634/1‑49 (MQ=255) tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg < 2:1694614/50‑1 (MQ=255) gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa > 1:1806193/1‑50 (MQ=255) gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa > 1:2588172/1‑50 (MQ=255) catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt > 1:3831922/1‑50 (MQ=255) catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt < 2:3278104/50‑1 (MQ=255) catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt < 2:1430690/50‑1 (MQ=255) catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt < 2:2707844/50‑1 (MQ=255) atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt > 1:4107703/1‑50 (MQ=255) atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt > 2:5256811/1‑50 (MQ=255) tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt < 1:2780547/50‑1 (MQ=255) gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt > 2:4333278/1‑50 (MQ=255) catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc > 2:4640105/1‑50 (MQ=255) catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc < 2:5160687/50‑1 (MQ=255) catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc < 2:693270/50‑1 (MQ=255) catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc < 2:2994274/50‑1 (MQ=255) catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc < 2:255201/50‑1 (MQ=255) atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt < 2:2196508/50‑1 (MQ=255) tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa > 2:2383396/1‑50 (MQ=255) tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa < 1:1704299/50‑1 (MQ=255) gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt > 2:3437400/1‑50 (MQ=255) cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt < 1:3729354/50‑1 (MQ=255) cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt > 1:1338198/1‑50 (MQ=255) | AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGGAT > NC_000913/118981‑119076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |