| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,843,412 | C→T | R12H (CGT→CAT) | hycH ← | formate hydrogenlyase assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,843,412 | 0 | C | T | 91.6% | 439.1 / 22.4 | 131 | R12H (CGT→CAT) | hycH | formate hydrogenlyase assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (3/8); new base T (50/70); total (53/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
ACCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGCC > NC_000913/2843364‑2843458 | ccTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATgg > 2:3344834/1‑50 (MQ=255) ctgctgCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGc > 2:4218646/1‑50 (MQ=255) ctgctgCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGc < 1:4993899/50‑1 (MQ=255) tgctgcGCCTCGGCGGGCGTGGCCTACTCCTTATCCATAAATTTATGGCt > 2:3010121/1‑50 (MQ=255) ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt > 1:119454/1‑48 (MQ=255) ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt < 2:931202/48‑1 (MQ=255) ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTc < 2:1898149/49‑1 (MQ=255) ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCa < 2:4635762/50‑1 (MQ=255) tgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAg > 2:1636354/1‑50 (MQ=255) gcgcCTCGGCGGGGGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGt > 2:2074453/1‑50 (MQ=255) gcgcCTCGGCGGGCGTGGCCTCGTTCTCATCAATAAATTTATGGCTCAGt > 1:1983455/1‑50 (MQ=255) cgcCTCGGCGGGCGTGGCATCGTTCTCATCAGTAAATTTATGGCTCAGt < 1:1267482/49‑1 (MQ=255) cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGtt < 1:4311323/50‑1 (MQ=255) cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAg < 2:4390062/48‑1 (MQ=255) cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGt < 1:769471/49‑1 (MQ=255) gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTg < 1:5264904/50‑1 (MQ=255) gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTg < 2:3918043/50‑1 (MQ=255) gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTg < 1:5354476/50‑1 (MQ=255) gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTg < 1:2960384/50‑1 (MQ=255) ccTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGa < 1:5065657/50‑1 (MQ=255) ccTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGa < 2:2083081/50‑1 (MQ=255) cTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGAc < 1:2065134/50‑1 (MQ=255) cTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGAc < 2:3471691/50‑1 (MQ=255) tCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt > 2:477706/1‑50 (MQ=255) ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt < 1:3825638/48‑1 (MQ=255) ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTg < 1:4616555/49‑1 (MQ=255) ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGa < 2:826740/50‑1 (MQ=255) tcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa < 2:4908287/49‑1 (MQ=255) gcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa < 2:304552/50‑1 (MQ=255) gcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa < 1:3178554/50‑1 (MQ=255) cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGatc < 2:4728913/50‑3 (MQ=255) cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa > 2:3599339/1‑49 (MQ=255) cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa > 2:5358378/1‑49 (MQ=255) cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa > 1:1858261/1‑49 (MQ=255) cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa > 1:2420533/1‑49 (MQ=255) cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAac < 1:1940346/50‑1 (MQ=255) gggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAaca > 2:4862990/1‑50 (MQ=255) gggcgTGGCAGCGTTCTCATCAATAAATTGACGGCTCAGTTGACTGAaca < 1:1245916/50‑1 (MQ=255) gtcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac > 2:4097073/3‑50 (MQ=255) ggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGCacac > 1:2823301/1‑50 (MQ=255) ggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac < 2:3704817/50‑1 (MQ=255) gcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcacc < 2:2525806/50‑1 (MQ=255) cgTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAAcacca < 2:3664455/50‑1 (MQ=255) gTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAAcaccac > 1:168799/1‑50 (MQ=255) tGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACaccacc > 1:3877950/1‑50 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt > 1:1177750/1‑50 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt > 1:4389909/1‑50 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt > 1:2216983/1‑50 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt > 1:5371394/1‑50 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt < 2:835627/50‑1 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt > 1:1638916/1‑50 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCt < 1:3652568/49‑1 (MQ=255) gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCt < 2:2267632/49‑1 (MQ=255) cATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCttt > 1:3370820/1‑50 (MQ=255) cATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCttt < 2:4172663/50‑1 (MQ=255) cATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCttt < 1:426649/50‑1 (MQ=255) aTCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt < 2:1533175/50‑1 (MQ=255) aTCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt < 2:1895235/50‑1 (MQ=255) aTCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt > 2:4349402/1‑50 (MQ=255) tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt > 2:4583456/1‑49 (MQ=255) tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTc < 1:4131546/50‑1 (MQ=255) tCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTc < 1:4075199/50‑1 (MQ=255) cGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa > 2:2709806/1‑50 (MQ=255) gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCGCCTTTTCa < 1:2082702/49‑1 (MQ=39) gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa < 1:4745893/49‑1 (MQ=255) gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc > 2:3707057/1‑50 (MQ=255) gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc > 2:1075349/1‑50 (MQ=255) gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc < 1:1506941/50‑1 (MQ=255) gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTGTCa < 2:2907069/49‑1 (MQ=39) ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCTCCTTTTCACt < 2:667743/50‑1 (MQ=255) ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt > 1:4354348/1‑50 (MQ=255) ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt < 1:3907117/50‑1 (MQ=255) ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt > 2:1150123/1‑50 (MQ=255) ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt > 2:3767211/1‑50 (MQ=255) ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt > 1:2648648/1‑50 (MQ=255) tcatcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCAt > 2:2939351/1‑50 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa < 1:5182051/47‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCAt < 2:3893378/48‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATc < 1:3481822/49‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATc < 2:5154901/49‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATc < 1:2428061/49‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATc < 2:4358965/49‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATc < 2:2678472/49‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg > 1:3229189/1‑50 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg < 2:4358284/50‑1 (MQ=255) atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg > 1:2948847/1‑50 (MQ=255) atcaATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg < 2:2605348/50‑1 (MQ=255) tcaATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg > 2:2358160/1‑49 (MQ=255) aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa > 2:3946571/1‑49 (MQ=255) tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc < 1:325880/50‑1 (MQ=255) tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc < 2:1912216/50‑1 (MQ=255) tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc < 2:2605324/50‑1 (MQ=255) aaaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcg > 2:2733086/1‑50 (MQ=255) aaaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcg > 2:2551723/1‑50 (MQ=255) aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgc > 1:5122010/1‑50 (MQ=255) aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcg < 1:4910005/49‑1 (MQ=255) aTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgcg < 1:5346489/50‑1 (MQ=255) aTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgcg > 1:1978717/1‑50 (MQ=255) aTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgcg > 2:2088910/1‑50 (MQ=255) ttATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGcc > 2:1248443/1‑50 (MQ=255) | ACCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGCC > NC_000913/2843364‑2843458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |