| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,213,803 | T→C | S253P (TCT→CCT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,213,803 | 0 | T | C | 78.7% | 309.7 / 85.4 | 136 | S253P (TCT→CCT) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (17/12); new base C (61/46); total (78/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 | |||||||||||
Only 100 of 138 total aligned reads displayed.
GCGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACTACCT > NC_000913/3213755‑3213852 | gCGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTc < 1:4513783/50‑1 (MQ=255) cGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCt < 2:4880597/50‑1 (MQ=255) cGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt > 2:4394454/1‑50 (MQ=255) gAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt > 2:3814508/1‑49 (MQ=255) gAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt > 1:4255975/1‑49 (MQ=255) gAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt > 1:2663190/1‑49 (MQ=255) gAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt > 1:44207/1‑49 (MQ=255) aaaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTg > 2:2245538/1‑49 (MQ=255) aaGGTCGCAGTCACGCTACCGCTCAGGAAGTGATCCTGAAACTGCCTg > 1:4406262/1‑48 (MQ=255) aaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTg > 2:536948/1‑48 (MQ=255) aaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTg > 2:5102247/1‑48 (MQ=255) aaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTg > 2:4097214/1‑48 (MQ=255) gTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTa > 1:540753/1‑50 (MQ=255) tCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAt > 1:3557651/1‑50 (MQ=255) tCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAt > 2:3723925/1‑50 (MQ=255) cGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt > 1:973158/1‑50 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTAtt < 1:214731/49‑1 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTc < 1:3886739/50‑1 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt > 2:2545908/1‑49 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTc < 1:13238/50‑1 (MQ=255) cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCa < 1:54012/50‑1 (MQ=255) cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCa < 2:5091436/50‑1 (MQ=255) cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa < 2:4390661/50‑1 (MQ=255) aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaa < 1:3860037/50‑1 (MQ=255) aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa > 2:3815567/1‑49 (MQ=255) gTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCaaa < 1:2802762/50‑1 (MQ=255) gTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaaa > 1:1828023/1‑50 (MQ=255) tCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAAc < 1:4888755/50‑1 (MQ=255) cACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaaa > 1:3653445/1‑48 (MQ=255) cACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaaa > 2:4907657/1‑48 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAg < 2:4073658/50‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACa < 1:5298793/49‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACa < 1:2889123/49‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACa < 1:1379826/49‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACa < 1:6980/49‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAg < 1:1025606/50‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAg < 1:3151383/50‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAg > 1:4960409/1‑50 (MQ=255) cGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGt > 2:1997604/1‑50 (MQ=255) cGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGt < 2:2474957/50‑1 (MQ=255) cGCTACCGCTCAGGAAGAAATCCTGAAACTGCCTGAAGTATTCAAACAGt > 2:3708590/1‑50 (MQ=39) gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt > 1:4193790/1‑50 (MQ=255) gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt < 2:1037963/50‑1 (MQ=255) gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt > 2:1153343/1‑50 (MQ=255) gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt < 1:3610402/50‑1 (MQ=255) cTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTc > 2:2709960/1‑50 (MQ=255) cTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTc < 1:193046/50‑1 (MQ=255) cTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTc < 2:3572938/50‑1 (MQ=255) cTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTc < 1:3071744/50‑1 (MQ=255) aCCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCg > 1:1796515/1‑50 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGc < 1:2000982/50‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc < 1:4288560/48‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc < 1:1563647/48‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc < 2:2256719/48‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCg < 1:2797451/49‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGc < 1:2004305/50‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGc > 2:1813604/1‑50 (MQ=255) tCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTgg > 1:3199575/1‑50 (MQ=255) tCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTgg > 1:4784471/1‑50 (MQ=255) tCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTgg > 2:1824008/1‑50 (MQ=255) tCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTgg > 1:3817291/1‑50 (MQ=255) tCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTgg > 1:3436620/1‑50 (MQ=255) cAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGt > 2:3940157/1‑50 (MQ=255) cAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGt > 2:1771441/1‑50 (MQ=255) aGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGt < 2:603190/49‑1 (MQ=255) aGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGt < 1:4479782/49‑1 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGt < 2:2536039/49‑1 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTg < 1:4210002/50‑1 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTg < 2:2205967/50‑1 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTg > 2:2446741/1‑50 (MQ=255) ggAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGc > 1:3741022/1‑50 (MQ=255) gAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGcc > 2:1738145/1‑50 (MQ=255) aaGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCg < 1:1098146/50‑1 (MQ=255) aaGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCg < 1:5090002/50‑1 (MQ=255) agagATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGa < 2:349693/50‑1 (MQ=255) gagaTCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCg > 1:1010234/1‑48 (MQ=255) gagaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCg > 1:5339751/1‑48 (MQ=255) gagaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGaa < 1:1257510/50‑1 (MQ=255) agaTCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 1:4686889/1‑50 (MQ=255) agaTCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 1:319046/1‑50 (MQ=255) agaTCCTGAAACTGCCTGAAGTCTTCAAACAGTTCCGCCTGGTGCCGAAg > 2:3510834/1‑50 (MQ=39) gaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGc < 2:570080/50‑1 (MQ=255) gaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGc < 2:4557593/50‑1 (MQ=255) aTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 1:3982975/1‑48 (MQ=255) tCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAg > 1:4074530/1‑50 (MQ=255) cTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGt < 1:2053740/49‑1 (MQ=255) cTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGt < 1:396327/49‑1 (MQ=255) tGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt > 1:315212/1‑50 (MQ=255) tGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt > 1:1016550/1‑50 (MQ=255) gAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt > 1:2655952/1‑49 (MQ=255) gAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt > 1:192012/1‑49 (MQ=255) gAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt > 1:1107746/1‑49 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt < 1:1990147/48‑1 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt < 2:1271937/48‑1 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa > 2:2720203/1‑50 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa > 1:5015459/1‑50 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa > 1:911828/1‑50 (MQ=255) aaCTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGAc > 2:2546084/1‑50 (MQ=255) aaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGAc < 1:1544531/50‑1 (MQ=255) cTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACTa < 2:3746046/50‑1 (MQ=255) | GCGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACTACCT > NC_000913/3213755‑3213852 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |