| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,477,150 | C→T | K90K (AAG→AAA) | fkpA ← | peptidyl‑prolyl cis‑trans isomerase FkpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,477,150 | 0 | C | T | 92.2% | 438.0 / 21.4 | 128 | K90K (AAG→AAA) | fkpA | peptidyl‑prolyl cis‑trans isomerase FkpA |
| Reads supporting (aligned to +/- strand): ref base C (6/4); new base T (58/60); total (64/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT > NC_000913/3477104‑3477196 | cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt < 1:1252954/49‑1 (MQ=255)cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt < 2:3985325/49‑1 (MQ=255)cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt < 1:2799167/49‑1 (MQ=255)cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa > 1:1477401/1‑50 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt > 2:3799351/1‑49 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt > 2:1764187/1‑49 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt > 2:144181/1‑49 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt > 2:3128078/1‑49 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc < 1:3621187/50‑1 (MQ=255) aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc < 1:721926/50‑1 (MQ=255) aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa < 1:3851285/50‑1 (MQ=255) tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg > 1:2209501/1‑50 (MQ=255) gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg > 1:4496691/1‑49 (MQ=255) cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc < 1:4242923/49‑1 (MQ=255) ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa > 1:5342234/1‑49 (MQ=255) ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa > 1:818661/1‑49 (MQ=255) tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa > 1:4772498/1‑50 (MQ=255) tAGAGTCTGTTCGATCTCTTGGTCGGAGGGTTTGCTCTTATCAGCaaagg < 2:2165488/50‑3 (MQ=255) tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt < 1:987577/49‑1 (MQ=255) tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt < 2:1173602/49‑1 (MQ=255) tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt < 1:3203426/49‑1 (MQ=255) tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg > 2:1383889/1‑50 (MQ=255) agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg > 1:1235617/1‑49 (MQ=255) gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa < 1:1825680/50‑1 (MQ=255) gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa > 1:3647852/1‑50 (MQ=255) gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCa > 2:465104/1‑50 (MQ=255) agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt < 1:395190/50‑1 (MQ=255) agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt < 1:2953494/50‑1 (MQ=255) agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCAt > 1:2117009/1‑50 (MQ=255) gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc < 2:1324136/50‑1 (MQ=255) gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc > 2:3576417/1‑50 (MQ=255) tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc < 1:4894736/50‑1 (MQ=255) cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt > 2:4923862/1‑50 (MQ=255) cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt > 2:3050658/1‑50 (MQ=255) tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt > 1:2466060/1‑49 (MQ=255) gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg > 1:939773/1‑49 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt < 2:4869963/47‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt < 1:4217427/47‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt < 1:1452041/47‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt < 2:2849634/47‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg < 1:3860338/48‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg < 1:3346062/48‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg < 1:1437067/48‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg < 1:4629749/48‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa < 1:328044/50‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa < 1:373481/49‑1 (MQ=255) ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa < 1:1503664/49‑1 (MQ=255) tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa > 2:4871255/1‑49 (MQ=255) gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:284706/1‑50 (MQ=255) gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 2:2239106/1‑50 (MQ=255) gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca < 1:492107/49‑1 (MQ=255) aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 2:4138833/1‑49 (MQ=255) aTCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAacac > 1:4589508/1‑49 (MQ=255) tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:1458004/1‑48 (MQ=255) tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 2:209427/1‑48 (MQ=255) tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:3813798/1‑48 (MQ=255) tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 2:3678759/1‑48 (MQ=255) tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:688198/1‑48 (MQ=255) tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac > 1:3449869/1‑48 (MQ=255) ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc > 2:2797443/1‑49 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac < 2:1152134/44‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc < 1:5296393/45‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa < 1:218049/46‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg < 1:209358/47‑1 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa > 1:1504714/1‑50 (MQ=255) ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa > 2:3276421/1‑50 (MQ=255) ttGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGc < 1:4651084/48‑1 (MQ=255) tGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt > 2:1237891/1‑50 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg < 1:977115/47‑1 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg < 2:1466729/47‑1 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa < 1:4725618/48‑1 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt > 1:61714/1‑49 (MQ=255) ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt > 2:4698209/1‑49 (MQ=255) ggTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGa < 1:1216369/48‑1 (MQ=255) gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc < 2:3303/49‑1 (MQ=255) gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa < 2:5196302/50‑1 (MQ=255) gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa < 1:1662162/50‑1 (MQ=255) tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg > 1:4172906/1‑50 (MQ=255) cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc > 2:486825/1‑50 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc < 1:2915118/49‑1 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt > 1:3231808/1‑50 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt < 2:1786805/50‑1 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt < 2:1307768/50‑1 (MQ=255) ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt < 2:1570473/50‑1 (MQ=255) gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt > 1:2782672/1‑49 (MQ=255) gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt > 1:1170251/1‑49 (MQ=255) agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa > 2:2941685/1‑50 (MQ=255) agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa > 2:4057603/1‑50 (MQ=255) agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa < 1:2226591/50‑1 (MQ=255) gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt < 1:1791077/50‑1 (MQ=255) tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt < 2:4567149/50‑1 (MQ=255) tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt < 1:2064730/50‑1 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt < 1:4346094/49‑1 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt < 2:4994086/49‑1 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt < 2:3382959/49‑1 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt < 2:2448597/49‑1 (MQ=255) tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa > 1:4054204/1‑50 (MQ=255) gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa < 1:3218233/49‑1 (MQ=255) gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt > 1:44617/1‑50 (MQ=255) ctctTATCAGCAAATGCATCCTGAACGCCAGCGATCAGCTGATCTTTAt < 2:2241732/49‑1 (MQ=255) | CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT > NC_000913/3477104‑3477196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |