Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 119,028 T→C V99A (GTC→GCC)  ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913119,0280TC100.0% 451.2 / NA 115V99A (GTC→GCC) ampD1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (68/47);  total (68/47)

Only 100 of 130 total aligned reads displayed.

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074
                                               |                                              
aaTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcct                                              <  1:2029838/50‑1 (MQ=255)
 aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGtctc                                             <  2:2163872/50‑1 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:3293868/1‑50 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:3879417/1‑50 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  2:1760443/50‑1 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:1758519/1‑50 (MQ=255)
   aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:3211270/1‑49 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:2673558/1‑48 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:1761259/1‑48 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:3735459/1‑48 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCa                                          <  1:761406/50‑1 (MQ=255)
     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  2:2545065/1‑50 (MQ=255)
     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  1:1934801/1‑50 (MQ=255)
     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  2:2551754/1‑50 (MQ=255)
      ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  2:2042612/1‑49 (MQ=255)
      ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  2:538113/1‑49 (MQ=255)
       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGt                                        <  1:2993380/49‑1 (MQ=255)
       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  1:810943/1‑50 (MQ=255)
       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       <  2:2427906/50‑1 (MQ=255)
        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  2:767715/1‑50 (MQ=255)
         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  2:739484/1‑49 (MQ=255)
         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                                     <  1:1634027/50‑1 (MQ=255)
         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                                     <  1:4567553/50‑1 (MQ=255)
          tATGTTCCTTTCGATAAACGTGTATGGCATGCGGGAGCCTCTCAGTAt                                      >  2:327568/1‑48 (MQ=38)
           aTGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   >  2:3046923/1‑50 (MQ=255)
            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  <  2:4438695/50‑1 (MQ=255)
             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:139464/1‑50 (MQ=255)
             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  2:4101839/1‑50 (MQ=255)
              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                >  2:2590293/1‑50 (MQ=255)
              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                >  1:2219542/1‑50 (MQ=255)
              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                <  2:1312797/50‑1 (MQ=255)
               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  1:2857170/1‑50 (MQ=255)
                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              >  1:1044290/1‑50 (MQ=255)
                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              >  2:2705593/1‑50 (MQ=255)
                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               <  1:3926275/49‑1 (MQ=255)
                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  2:1080895/50‑1 (MQ=255)
                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  2:1396179/50‑1 (MQ=255)
                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  2:769507/50‑1 (MQ=255)
                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              <  2:4182852/49‑1 (MQ=255)
                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:2672739/1‑49 (MQ=255)
                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:1104696/1‑49 (MQ=255)
                   ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            >  2:17201/1‑49 (MQ=255)
                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                          >  1:1558732/1‑50 (MQ=255)
                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:3023942/1‑50 (MQ=255)
                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  2:3329539/1‑50 (MQ=255)
                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:1187907/49‑1 (MQ=255)
                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:1654599/49‑1 (MQ=255)
                       aTAAACGTGTATGGCATGTGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  2:1578263/1‑50 (MQ=38)
                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  1:3709903/1‑50 (MQ=255)
                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  2:1498721/1‑50 (MQ=255)
                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      >  1:4346815/1‑50 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCTCGAACGCTg                     >  2:43549/1‑49 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     <  2:2591050/49‑1 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  2:2396436/1‑49 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:349463/1‑49 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:3568724/1‑49 (MQ=255)
                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    >  1:1513983/1‑49 (MQ=255)
                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCa                   <  1:915350/50‑1 (MQ=255)
                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  2:3115885/1‑50 (MQ=255)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCact                 <  2:3751803/50‑3 (MQ=39)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  2:46477/1‑50 (MQ=255)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:978232/1‑50 (MQ=255)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  2:1315825/1‑50 (MQ=255)
                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:1364378/1‑49 (MQ=255)
                               gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               >  1:109509/1‑50 (MQ=255)
                               gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               >  1:1855152/1‑50 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               <  1:2586609/49‑1 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  2:1830388/1‑50 (MQ=255)
                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             <  1:2898896/50‑1 (MQ=255)
                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  1:3680983/1‑50 (MQ=255)
                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             <  2:2648022/50‑1 (MQ=255)
                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:2958498/1‑50 (MQ=255)
                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:1250673/1‑50 (MQ=255)
                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:593588/1‑50 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          >  2:2836739/1‑50 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          <  2:3174187/50‑1 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          >  2:3618741/1‑50 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt           <  1:838395/49‑1 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt           <  2:1051622/49‑1 (MQ=255)
                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         <  2:4394848/50‑1 (MQ=255)
                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:1177721/1‑50 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:1434123/1‑49 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  2:3735923/1‑49 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:107739/1‑49 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  2:2841737/1‑49 (MQ=255)
                                       tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa       <  1:204889/50‑1 (MQ=255)
                                        gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      >  2:4397613/1‑50 (MQ=255)
                                        gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      >  1:4326231/1‑50 (MQ=255)
                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt     >  1:3107798/1‑50 (MQ=255)
                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt     <  2:3052324/50‑1 (MQ=255)
                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt     <  1:3758733/50‑1 (MQ=255)
                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  2:1504002/49‑1 (MQ=255)
                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  2:1569435/49‑1 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    <  1:3024673/50‑1 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    <  1:4205724/50‑1 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    <  2:530927/50‑1 (MQ=255)
                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:3076556/50‑1 (MQ=255)
                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:3673776/50‑1 (MQ=255)
                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   >  1:2253543/1‑49 (MQ=255)
                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   >  2:1125896/1‑49 (MQ=255)
                                               |                                              
AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑