Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,270,811 C→T V347M (GTG→ATG)  chaA ← Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,8110CT100.0% 500.6 / NA 124V347M (GTG→ATG) chaANa(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/79);  total (45/79)

Only 100 of 124 total aligned reads displayed.

CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857
                                               |                                              
catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  2:3110933/1‑50 (MQ=255)
catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:2327068/1‑50 (MQ=255)
 atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  2:4482457/1‑49 (MQ=255)
 atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:4164656/1‑49 (MQ=255)
  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:220268/1‑48 (MQ=255)
  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  2:3461916/50‑1 (MQ=255)
    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           <  2:4407218/49‑1 (MQ=255)
    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                          <  1:3670289/50‑1 (MQ=255)
    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                          <  1:18578/50‑1 (MQ=255)
     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  2:1973393/50‑1 (MQ=255)
     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  2:1581321/50‑1 (MQ=255)
     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:2062145/50‑1 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  2:3030909/50‑1 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:854617/50‑1 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:1447256/50‑1 (MQ=255)
       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  1:635251/50‑1 (MQ=255)
       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  1:125424/50‑1 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      <  2:672200/49‑1 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      <  2:3114877/49‑1 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      <  1:3148504/49‑1 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     >  2:3563503/1‑50 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:2465273/50‑1 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:3514444/50‑1 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    >  1:4405887/1‑50 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  2:1528009/50‑1 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:3357738/50‑1 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  2:3705409/50‑1 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  2:3126044/50‑1 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  2:422785/50‑1 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  2:3047264/50‑1 (MQ=255)
            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  <  1:3214691/50‑1 (MQ=255)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCggag                                >  1:2584738/1‑48 (MQ=39)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:867947/50‑1 (MQ=255)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  2:2151227/50‑1 (MQ=255)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:2838475/1‑50 (MQ=255)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:659100/50‑1 (MQ=255)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  2:4538413/50‑1 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  2:831249/50‑1 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  1:2446993/50‑1 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:3812753/1‑49 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:712746/1‑49 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:4063950/1‑49 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:3574144/1‑49 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:2314636/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:3727305/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:3254625/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                              <  1:1550339/50‑1 (MQ=255)
                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:656312/1‑47 (MQ=255)
                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:2589401/50‑1 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  2:4538803/1‑49 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:3922874/1‑49 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                           <  1:1111334/50‑1 (MQ=255)
                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          <  2:2202302/50‑1 (MQ=255)
                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:916474/50‑1 (MQ=255)
                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:134498/50‑1 (MQ=255)
                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  2:3512329/50‑1 (MQ=255)
                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:2561249/1‑48 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:4305273/1‑47 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                       <  2:1715737/50‑1 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:245762/1‑46 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:2292553/1‑46 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:2084142/1‑46 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  2:4468669/1‑49 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  2:434280/1‑49 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:2735778/50‑1 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:3901644/50‑1 (MQ=255)
                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                    >  2:2399006/1‑50 (MQ=255)
                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                    <  1:1726231/50‑1 (MQ=255)
                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   <  2:1900362/50‑1 (MQ=255)
                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  2:946968/50‑1 (MQ=255)
                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  2:2941534/50‑1 (MQ=255)
                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  1:4262124/50‑1 (MQ=255)
                              cgctgcgcCATTGACCATGTTAGTACGGCCGGTGGAGAAGGAGATATGGc                <  1:2518265/50‑1 (MQ=38)
                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               <  2:1303105/50‑1 (MQ=255)
                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  2:4397127/1‑49 (MQ=255)
                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:3147356/1‑49 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  2:3462982/48‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:1964983/49‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            >  1:2486094/1‑49 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:1701540/49‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  2:3388980/50‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  2:4531315/50‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  2:702084/50‑1 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  2:1476679/50‑1 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  1:2993612/50‑1 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:1037536/1‑50 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         <  1:2813185/50‑1 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         <  1:3096815/50‑1 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  2:1705850/1‑50 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:3389817/50‑1 (MQ=255)
                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:917048/50‑1 (MQ=255)
                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  2:1483137/50‑1 (MQ=255)
                                         tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:2651016/1‑48 (MQ=255)
                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     <  1:2625936/49‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  2:392713/50‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  2:1762935/50‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  2:4307996/49‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  2:513650/49‑1 (MQ=255)
                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  1:3966759/1‑50 (MQ=255)
                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  1:1303794/50‑1 (MQ=255)
                                               |                                              
CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑