Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,742,755 G→C P163R (CCG→CGG)  ribC ← riboflavin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,742,7550GC100.0% 465.1 / NA 116P163R (CCG→CGG) ribCriboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (64/52);  total (64/52)

Only 100 of 116 total aligned reads displayed.

GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801
                                               |                                              
gTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGa                                              <  1:1095288/50‑1 (MQ=255)
gTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGa                                              <  2:67372/50‑1 (MQ=255)
 tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                             >  2:44962/1‑50 (MQ=255)
 tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                             <  1:3175679/50‑1 (MQ=255)
   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            <  1:447996/49‑1 (MQ=255)
   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            <  2:526448/49‑1 (MQ=255)
   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            <  1:4044405/49‑1 (MQ=255)
   cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  2:2735754/1‑49 (MQ=255)
    gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           >  2:1851097/1‑49 (MQ=255)
    gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  2:1403643/50‑1 (MQ=255)
     ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                         <  2:388257/50‑1 (MQ=255)
     ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          >  1:4561798/1‑49 (MQ=255)
      cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:3570423/1‑50 (MQ=255)
      cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        <  2:4129801/50‑1 (MQ=255)
       aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       >  2:3859152/1‑50 (MQ=255)
        aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  2:3602710/1‑50 (MQ=255)
         gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  2:3786006/1‑49 (MQ=255)
         gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  2:3980713/1‑49 (MQ=255)
         gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:3845280/1‑49 (MQ=255)
          ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    <  1:4020377/50‑1 (MQ=255)
          ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:4138130/1‑50 (MQ=255)
          ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:2354855/1‑50 (MQ=255)
          ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  2:1448390/1‑50 (MQ=255)
          ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:1050822/1‑50 (MQ=255)
              ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                 >  1:294498/1‑49 (MQ=255)
               tcttcCCAAGAGTCTTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  2:1311546/49‑1 (MQ=255)
               tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                >  1:858317/1‑49 (MQ=255)
                cttcCCAAGAGTCGCGCGTTCCAGTGTTTCCCGAATTAAATGGACCCaa                               >  1:108908/1‑49 (MQ=38)
                 ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              >  1:3072966/1‑49 (MQ=255)
                  tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            >  2:802494/1‑50 (MQ=255)
                  tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            >  1:2278495/1‑50 (MQ=255)
                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            <  2:2144956/49‑1 (MQ=255)
                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            <  2:3120599/49‑1 (MQ=255)
                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                             <  2:2285176/48‑1 (MQ=255)
                   cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              <  1:1570936/47‑1 (MQ=255)
                    ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                          <  2:683068/50‑1 (MQ=255)
                    ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                          >  2:2265310/1‑50 (MQ=255)
                    ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                          >  2:3785105/1‑50 (MQ=255)
                    ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:3122788/49‑1 (MQ=255)
                     cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:285090/1‑50 (MQ=255)
                     cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         <  1:532625/50‑1 (MQ=255)
                      aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        >  1:2151182/1‑50 (MQ=255)
                      aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        >  1:2385968/1‑50 (MQ=255)
                      aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        >  1:1983536/1‑50 (MQ=255)
                      aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        <  2:4226226/50‑1 (MQ=255)
                      aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        >  1:3801956/1‑50 (MQ=255)
                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                       >  2:588535/1‑50 (MQ=255)
                       agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGC‑AAAACGCGtt                      >  2:2503305/1‑50 (MQ=255)
                         agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  2:3126294/50‑1 (MQ=255)
                         agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     >  1:816483/1‑50 (MQ=255)
                         agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:177559/50‑1 (MQ=255)
                         agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  2:686016/50‑1 (MQ=255)
                          gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    <  1:3707107/50‑1 (MQ=255)
                          gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    <  2:4539950/50‑1 (MQ=255)
                           tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                   >  2:3437309/1‑50 (MQ=255)
                           tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                   >  1:574782/1‑50 (MQ=255)
                             gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  <  2:581922/49‑1 (MQ=255)
                             gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:3277702/1‑50 (MQ=255)
                             gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:964949/1‑50 (MQ=255)
                             gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  2:4173733/1‑50 (MQ=255)
                             gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  2:2705231/1‑50 (MQ=255)
                              tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:4341821/1‑49 (MQ=255)
                              tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  2:4079442/1‑49 (MQ=255)
                              tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  2:3039102/1‑49 (MQ=255)
                               gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa               >  2:3844748/1‑50 (MQ=255)
                                cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa               <  1:2255139/49‑1 (MQ=255)
                                cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              >  2:1626740/1‑50 (MQ=255)
                                cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              >  1:3334611/1‑50 (MQ=255)
                                cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              <  1:1700716/50‑1 (MQ=255)
                                 gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGaat             <  1:3746936/50‑3 (MQ=255)
                                 gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             >  1:4103467/1‑50 (MQ=255)
                                 gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             >  1:4534041/1‑50 (MQ=255)
                                 gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:2956993/50‑1 (MQ=255)
                                 gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             >  2:3602884/1‑50 (MQ=255)
                                  ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            >  2:1908851/1‑50 (MQ=255)
                                  ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            <  2:1828879/50‑1 (MQ=255)
                                   tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           >  2:1449088/1‑50 (MQ=255)
                                    ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  2:3113242/49‑1 (MQ=255)
                                    ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  2:4551488/49‑1 (MQ=255)
                                     cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg          <  1:2944134/49‑1 (MQ=255)
                                      aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        <  2:1382667/50‑1 (MQ=255)
                                      aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGCGACTTCGcc        >  2:1280861/1‑50 (MQ=255)
                                       gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  2:2555956/1‑49 (MQ=255)
                                        tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      >  1:3430467/1‑50 (MQ=255)
                                        tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      <  2:4112560/50‑1 (MQ=255)
                                        tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      >  2:4339093/1‑50 (MQ=255)
                                         gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:474833/1‑50 (MQ=255)
                                         gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     >  1:1302604/1‑50 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     <  2:1878416/49‑1 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     <  2:512509/49‑1 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:1240955/1‑50 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:3693282/1‑50 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:1574257/50‑1 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:686985/50‑1 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:3048573/50‑1 (MQ=255)
                                          tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:2086482/1‑50 (MQ=255)
                                           ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   >  1:167722/1‑50 (MQ=255)
                                            tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   <  2:219170/49‑1 (MQ=255)
                                            tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt  >  2:3071015/1‑50 (MQ=255)
                                            tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt  >  1:2738494/1‑50 (MQ=255)
                                               |                                              
GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑