Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 257,794 T→C intergenic (+23/‑35) frsA → / → crl fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913257,7940TC100.0% 682.9 / NA 165intergenic (+23/‑35)frsA/crlfermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (102/63);  total (102/63)

Only 100 of 165 total aligned reads displayed.

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840
                                               |                                              
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  2:2893339/50‑1 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  2:3692456/50‑1 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              >  1:1775219/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  2:237165/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:1302599/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:1683231/1‑50 (MQ=255)
  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  2:3938784/1‑49 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                            >  1:1038070/1‑49 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                            <  2:2324541/49‑1 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                            >  1:2795158/1‑49 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  2:1776140/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  2:153908/50‑1 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:1174706/1‑50 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        <  2:300969/49‑1 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:3599583/1‑49 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:4198359/50‑1 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:3017563/50‑1 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:664046/1‑48 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:200835/1‑48 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa                                      <  2:3321155/50‑1 (MQ=255)
         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     >  2:2516615/1‑50 (MQ=255)
         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     <  1:3335163/50‑1 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  2:4230843/1‑50 (MQ=255)
           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   <  2:1635118/50‑1 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  2:3593127/50‑1 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  2:1132454/50‑1 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  1:3255149/50‑1 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  2:687225/1‑49 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  2:3765402/1‑49 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  2:3861187/1‑49 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  1:1088525/1‑49 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGc                                 <  1:4055369/50‑1 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                >  1:1572100/1‑50 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                >  2:1050888/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:526194/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               <  1:4173270/50‑1 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  2:3983583/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               <  2:3069022/50‑1 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:1486448/1‑50 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:3136188/1‑50 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:897194/1‑50 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  2:3731923/1‑50 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                           <  1:2726294/50‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                           <  1:2608061/50‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                           <  2:4141436/50‑1 (MQ=255)
                    gtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:3210549/1‑50 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:3976996/1‑49 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  2:2051952/1‑49 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          <  1:2030426/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  2:2788121/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:2363539/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:673068/1‑50 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:1371356/1‑50 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:2881947/1‑50 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  2:1536193/49‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:2441736/1‑49 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:708366/1‑49 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:8995/1‑48 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:2996221/1‑48 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:3541965/1‑48 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGga                     <  2:2049960/50‑1 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:4177280/1‑47 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                    <  2:3283773/50‑1 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:221995/1‑46 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:256440/1‑46 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:3588854/1‑46 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  2:470171/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:2236706/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  2:1638993/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:268908/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt                  <  1:4481736/50‑1 (MQ=255)
                             ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  2:2156602/1‑48 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                 <  2:3688742/49‑1 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                 <  1:3162392/49‑1 (MQ=255)
                              tGCTAAATTTTGCCAAGCTGGTAAAACAGTTGCATCACAACAGGAGATa                 <  2:404784/49‑1 (MQ=39)
                               gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                <  2:769029/49‑1 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  1:1837318/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:3920897/49‑1 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  1:1880585/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               >  2:4142629/1‑49 (MQ=255)
                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             >  1:1398492/1‑50 (MQ=255)
                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             >  1:3234363/1‑50 (MQ=255)
                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             >  2:4330449/1‑50 (MQ=255)
                                  aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            >  1:1002492/1‑50 (MQ=255)
                                  aaaTTTTCCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            >  2:2661930/1‑50 (MQ=39)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             <  2:2373094/48‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  2:1798315/49‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:3950881/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:1991067/1‑50 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         >  1:3192592/1‑50 (MQ=255)
                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  2:1884907/1‑50 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:1138976/1‑49 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  2:3091721/1‑49 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  1:3413910/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     >  2:3425530/1‑50 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     <  1:4308074/49‑1 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     <  1:1090496/49‑1 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    >  2:1556924/1‑50 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGGTAc    <  2:766225/50‑1 (MQ=39)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2042734/1‑50 (MQ=255)
                                               |                                              
CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑