Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,477,150 C→T K90K (AAG→AAA)  fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,1500CT100.0% 647.7 / NA 163K90K (AAG→AAA) fkpApeptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (80/83);  total (80/83)

Only 100 of 167 total aligned reads displayed.

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196
                                              |                                              
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  2:4260743/49‑1 (MQ=255)
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  2:184208/49‑1 (MQ=255)
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  1:1336617/1‑50 (MQ=255)
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  2:1878469/1‑50 (MQ=255)
 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:2539051/49‑1 (MQ=255)
 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:1324580/1‑50 (MQ=255)
  attGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTAGCTTTTAt                                            >  1:2608339/3‑49 (MQ=255)
  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  2:813753/1‑49 (MQ=255)
  aaTGCTTGCAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCttttct                                            >  2:3316101/1‑47 (MQ=255)
   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  2:1346357/1‑48 (MQ=255)
   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:1096655/50‑1 (MQ=255)
   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:1979886/50‑1 (MQ=255)
    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  2:4480818/1‑50 (MQ=255)
    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  2:4230185/1‑50 (MQ=255)
     gCTTGCAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:4135736/1‑49 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:4460444/49‑1 (MQ=255)
       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  2:3662589/1‑49 (MQ=255)
        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  2:1572470/1‑50 (MQ=255)
        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:1342074/1‑50 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:615708/48‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    >  1:1652838/1‑50 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    >  2:4071903/1‑50 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:1729268/49‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  2:870388/50‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  2:1110934/50‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:3548408/50‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:237125/50‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  2:4345072/1‑50 (MQ=255)
           agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  2:3506292/1‑49 (MQ=255)
            gagTCTGTTCGGTCTCTTGGTCGGAGAGTGTGCTTTTATCAGCAAATGc                                  <  1:1244078/49‑1 (MQ=255)
            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  2:2287239/49‑1 (MQ=255)
            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  2:2019762/49‑1 (MQ=255)
             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                >  2:2760394/1‑50 (MQ=255)
             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  2:1978118/50‑1 (MQ=255)
             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                >  1:1802431/1‑50 (MQ=255)
             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  2:4382256/50‑1 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  2:4143423/1‑50 (MQ=255)
               tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                              <  1:1400780/50‑1 (MQ=255)
                cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:2915016/1‑50 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:3448594/48‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  2:1026706/48‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:1446390/50‑1 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  1:2133180/1‑50 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  2:3295275/1‑50 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  2:4355499/1‑50 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                         <  1:2859670/49‑1 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                         <  1:4337922/49‑1 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:3393246/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:3783176/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:369148/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:826228/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:315434/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        <  2:4211155/49‑1 (MQ=255)
                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:2192866/1‑48 (MQ=255)
                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:237495/1‑48 (MQ=255)
                         ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    >  2:1097011/1‑50 (MQ=255)
                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:2299250/1‑50 (MQ=255)
                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:802102/1‑50 (MQ=255)
                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  2:3859718/1‑50 (MQ=255)
                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:2165320/1‑49 (MQ=255)
                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  2:2730576/1‑49 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:845291/45‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  2:2932480/48‑1 (MQ=255)
                             tGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  1:4532624/1‑50 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 <  1:4018158/48‑1 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:3940171/49‑1 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  2:1426975/49‑1 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  1:663861/1‑49 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  2:4545492/1‑49 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  2:3341984/1‑49 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:1926729/49‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:71826/49‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:2985122/50‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:1855517/50‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:4445344/50‑1 (MQ=255)
                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  2:2887196/1‑50 (MQ=255)
                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  2:1522634/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  2:3373623/49‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  2:695910/50‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:4542747/50‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  2:2446907/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  2:4448285/50‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  2:2193925/1‑50 (MQ=255)
                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:420825/50‑1 (MQ=255)
                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  1:4454280/50‑1 (MQ=255)
                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  1:1019547/50‑1 (MQ=255)
                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         >  2:2625357/1‑50 (MQ=255)
                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  2:2585494/50‑1 (MQ=255)
                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        <  2:3478854/50‑1 (MQ=255)
                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       <  2:3571938/50‑1 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:2979430/1‑50 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:2481414/49‑1 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  2:3861995/49‑1 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  2:3136246/49‑1 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   >  2:187104/1‑50 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   >  1:3635036/1‑50 (MQ=255)
                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  2:4220929/1‑50 (MQ=255)
                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:4290679/1‑50 (MQ=255)
                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  2:3961780/1‑50 (MQ=255)
                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  2:677441/1‑50 (MQ=255)
                                              |                                              
CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑