Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	414.4 / NA	88	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (47/41);  total (47/41)

GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765140‑765233                                                  |                                             gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                              <  1:1837700/50‑1 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                              <  1:3501368/50‑1 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                              <  2:4768206/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                             <  2:3448879/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                              >  2:1647961/1‑49 (MQ=255)   cTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                            >  1:3228008/1‑49 (MQ=255)    ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                            >  1:4099832/1‑48 (MQ=255)     tttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                            >  1:1259231/1‑47 (MQ=255)      ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                           <  2:530580/48‑3 (MQ=255)      ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                           <  1:4391012/48‑3 (MQ=255)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       >  1:4114120/1‑49 (MQ=38)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       <  2:1148003/49‑1 (MQ=38)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                                      <  1:4938692/49‑1 (MQ=38)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                     >  2:4507922/1‑50 (MQ=39)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                     >  2:4158053/1‑50 (MQ=39)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                     >  2:4856409/1‑50 (MQ=39)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    >  1:1486814/1‑50 (MQ=39)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    >  2:2343886/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  1:2333170/50‑1 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   >  2:4931217/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  1:4788201/50‑1 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  1:5534203/50‑1 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   >  1:4536277/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  2:2915110/50‑1 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  1:3687514/50‑1 (MQ=39)            gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                  >  2:4822215/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 >  2:2482514/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                  <  1:1887217/49‑1 (MQ=38)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                  <  2:4828155/49‑1 (MQ=38)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 >  1:3630320/1‑49 (MQ=38)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 >  1:2304991/1‑49 (MQ=38)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 >  2:3025682/1‑49 (MQ=38)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 >  2:4930044/1‑49 (MQ=38)               tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                               >  1:3664893/1‑50 (MQ=39)               tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                               >  1:1829926/1‑50 (MQ=39)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                <  2:4497532/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                <  1:3775631/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                               <  2:1598081/49‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                               <  2:4837161/49‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                               <  2:486171/49‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                              >  1:2654396/1‑50 (MQ=39)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                              >  2:3393176/1‑50 (MQ=39)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                              >  1:4813405/1‑50 (MQ=39)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                             <  1:5255737/50‑1 (MQ=39)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                              >  1:3733983/1‑49 (MQ=38)                  cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  2:41702‑M1/1‑50 (MQ=255)                  cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  1:2899921‑M1/1‑50 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAt                           <  1:4901727‑M1/50‑2 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAt                           <  2:4450588‑M1/50‑2 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            <  1:5459985‑M1/49‑1 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            <  1:4721224‑M1/49‑1 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            <  1:1471900‑M1/49‑1 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                          <  1:1210292‑M1/50‑3 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                          <  2:245942‑M1/50‑3 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  1:401316‑M1/1‑48 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  1:2015369‑M1/1‑48 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         <  1:577091‑M1/50‑4 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         >  2:1749720‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         >  1:1251453‑M1/1‑47 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        <  2:3850489‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        <  1:5641200‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        <  1:696198‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        <  1:3865622‑M1/50‑5 (MQ=255)                      atgagTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         >  1:2437331‑M1/1‑46 (MQ=255)                        gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                      >  2:1533300‑M1/1‑44 (MQ=255)                        gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                      >  1:1294961‑M1/1‑44 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                     >  2:1948896‑M1/1‑43 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                     >  1:3391962‑M1/1‑43 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                     <  2:3542271‑M1/50‑8 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                    <  1:3148646‑M1/50‑9 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                     >  1:4870495‑M1/1‑42 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCAGCGGTATTAtagcaatc                    <  2:717837‑M1/50‑9 (MQ=255)                           tttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                    >  1:644029‑M1/1‑41 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                   <  1:1929916‑M1/49‑10 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                   <  2:1078246‑M1/49‑10 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                >  1:1975806‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                 <  2:831662‑M1/49‑12 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                 <  2:1836603‑M1/49‑12 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                 <  2:2910796‑M1/49‑12 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc               >  2:1393361‑M1/1‑37 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc               >  2:1102438‑M1/1‑37 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc               >  2:3422912‑M1/1‑37 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag           >  1:2067722‑M1/1‑33 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccga             <  1:3261355‑M1/48‑16 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:2934536‑M1/1‑32 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          <  2:3038085‑M1/50‑19 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:130419‑M1/1‑32 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:549711‑M1/1‑31 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca        >  2:4527266‑M1/1‑30 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca        >  1:3053576‑M1/1‑30 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca      >  1:770307‑M1/1‑28 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca        <  1:3754827‑M1/48‑21 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca        <  2:1999471‑M1/48‑21 (MQ=255)                                         cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca      >  2:1341399‑M1/1‑27 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata    >  2:5094727‑M1/1‑26 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat     <  2:3550679‑M1/49‑24 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat   <  1:1499538‑M1/50‑26 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg  >  2:5468385‑M1/1‑24 (MQ=255)                                                  |                                             GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765140‑765233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑