| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,957 | (G)6→7 | coding (71/1386 nt) | yifK → | putative transporter YifK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 93.2% | 264.7 / 12.6 | 73 | coding (65/1386 nt) | yifK | putative transporter YifK |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (29/39); total (32/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
CCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTA > NC_000913/3980902‑3980995 | ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCt < 2:31492/50‑1 (MQ=255)ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCt < 1:3488811/50‑1 (MQ=255)ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCt < 1:3316704/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑g < 1:4796889/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑g > 1:5047199/1‑50 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑g < 2:2150792/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑g < 1:2659910/50‑1 (MQ=255) cAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑g > 1:2469122/1‑50 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg < 1:4054998/50‑1 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg < 1:190281/50‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg < 1:4319658/50‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg < 2:5230450/50‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg > 2:3857314/1‑50 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg < 2:1503010/50‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg < 2:3452596/50‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg > 1:259286/1‑50 (MQ=255) gCTACAGCGTGGGCTGGAAGCTCGGCATATCGAACTCAACGCCCC‑gg‑gg‑‑g < 2:5843511/50‑1 (MQ=38) gCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑g > 1:4680032/1‑50 (MQ=255) gCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑g < 2:2908885/50‑1 (MQ=255) gCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑g > 1:1665342/1‑50 (MQ=255) cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑gg > 1:1589877/1‑50 (MQ=255) cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑gg < 1:3430063/50‑1 (MQ=255) tACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑ggg > 2:5074987/1‑49 (MQ=255) tACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑ggg > 1:5144699/1‑49 (MQ=255) aCAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑ggg > 2:3362877/1‑48 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑gggc < 1:2353164/49‑3 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑ggg < 2:2522617/48‑2 (MQ=255) cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gg‑‑ggg < 1:3205806/48‑2 (MQ=255) aGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GG‑‑GGCaac < 2:4406052/50‑3 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCa < 2:3245626/50‑1 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCa > 1:131049/1‑50 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCa < 2:2003452/50‑1 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCa < 1:4542300/50‑1 (MQ=255) gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCAt > 1:5713703/1‑50 (MQ=255) gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCAt > 1:4285588/1‑50 (MQ=255) gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCAt > 1:4275231/1‑50 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCAtt < 1:2753696/50‑1 (MQ=255) gggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTg > 2:5352750/1‑50 (MQ=255) gggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTg > 2:5237248/1‑50 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTgg < 2:607451/50‑1 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTg < 2:4584017/49‑1 (MQ=39) ggCTGGAAGCGCGACATATCGAACTCACCGCCCTGGG‑GG‑‑GGCACCATTg < 2:1734011/49‑1 (MQ=25) gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGc < 2:3951169/50‑1 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGc < 2:4937033/50‑1 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGc > 2:5101660/1‑50 (MQ=255) cTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCg > 1:3526523/1‑50 (MQ=255) cTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCg > 2:2879584/1‑50 (MQ=255) tGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGt > 1:2233269/1‑50 (MQ=255) tGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GG‑‑GGCACCATTGGCGTc > 2:682435/1‑50 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTc > 1:2127362/1‑50 (MQ=255) ggAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTc > 1:4864326/1‑50 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTc < 2:4193185/49‑1 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCg < 2:4182654/50‑1 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCg > 1:590377/1‑50 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCg < 1:1583496/50‑1 (MQ=255) aaGCTCGACATATCGAACTCATCGCCCT‑GGTGG‑‑GGCACCATTGGCGTCgg > 2:3569922/1‑50 (MQ=255) aGCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGc > 2:2439133/1‑50 (MQ=255) gCTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGcc < 2:1548391/50‑1 (MQ=255) cTCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCt < 1:544731/50‑1 (MQ=255) cTCGACATATCGAACTCATCGCCCT‑GG‑GG‑‑GGCACCATTGGCGTCGGCCTg < 2:1589877/50‑1 (MQ=255) cTCGACATAACGAACTAATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCt < 2:2103636/50‑1 (MQ=255) tCGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTg > 2:3060029/1‑50 (MQ=255) cGACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGt > 2:1167167/1‑50 (MQ=255) cGACATATCGAACTCATCGCCCT‑GG‑GGGTGGCACCATTGGCGTCGGCCTg > 2:5228835/1‑50 (MQ=255) gACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGtt < 2:639576/50‑1 (MQ=255) gACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGt < 1:3208023/49‑1 (MQ=39) gACATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGt < 2:4799592/49‑1 (MQ=39) aCATATCGAACTCATCGCCCTGGG‑GG‑‑GGCGCCATTGGCGTCGGCCTGttt < 1:2873105/50‑1 (MQ=255) aCATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGttt < 1:800507/50‑1 (MQ=255) aCATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGtt > 1:1041462/1‑49 (MQ=39) cATATCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTa > 1:3301346/1‑50 (MQ=255) atatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTAt > 2:3061483/1‑50 (MQ=255) atatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTAt < 1:1676954/50‑1 (MQ=255) atatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTAt < 2:5232886/50‑1 (MQ=255) atatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTAt > 1:4159434/1‑50 (MQ=255) atatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTAt > 2:1593989/1‑50 (MQ=255) atatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTAt > 2:3616987/1‑50 (MQ=255) tatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATg < 2:817000/50‑1 (MQ=255) tatCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATg > 1:631916/1‑50 (MQ=255) atCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATgg > 1:1627314/1‑50 (MQ=255) tCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATggg < 2:3017893/50‑1 (MQ=255) tCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATggg < 1:2025956/50‑1 (MQ=255) tCGAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATggg < 1:3556418/50‑1 (MQ=255) gAACTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATggggg < 1:3492035/50‑1 (MQ=255) aaCTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgc < 2:5165795/50‑1 (MQ=255) aaCTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGCGGgc < 2:2283593/50‑1 (MQ=255) aCTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgcc < 1:3604918/50‑1 (MQ=255) aCTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgcc < 1:4632910/50‑1 (MQ=255) aCTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgcc < 1:4703055/50‑1 (MQ=255) aCTCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgcc < 1:1399039/50‑1 (MQ=255) tCATCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 1:5621537/1‑50 (MQ=255) tCATCGCCCTGGG‑GG‑‑GGCACCATTGCCGTCGGCCTGCTTATGGGcgccgc > 2:224844/1‑50 (MQ=255) aTCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc < 2:5445070/49‑1 (MQ=39) aTCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 2:3485792/48‑1 (MQ=39) aTCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:2629266/50‑1 (MQ=255) tCGCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg < 2:1986115/50‑1 (MQ=255) gCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGt < 1:3738736/49‑1 (MQ=39) gCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa > 2:3266220/1‑50 (MQ=255) gCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa < 1:2171797/50‑1 (MQ=255) gCCCTGGG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa > 1:593528/1‑50 (MQ=255) | CCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GG‑‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTA > NC_000913/3980902‑3980995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |