Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 988,499 G→A P163S (CCA→TCA)  asnS ← asparagine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913988,4990GA100.0% 644.9 / NA 161P163S (CCA→TCA) asnSasparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (54/107);  total (54/107)

Only 100 of 161 total aligned reads displayed.

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545
                                               |                                              
gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  2:4618256/1‑50 (MQ=255)
gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              <  2:5583514/50‑1 (MQ=255)
 cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             <  1:4846248/50‑1 (MQ=255)
 cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             <  2:4626110/50‑1 (MQ=255)
  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            <  2:4484845/50‑1 (MQ=255)
  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  2:5264122/1‑50 (MQ=255)
  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGGAATCAGGGACGtt                                            >  2:805419/1‑50 (MQ=255)
    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:1223878/1‑49 (MQ=255)
    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                          <  2:4904195/50‑1 (MQ=255)
    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                          <  2:5846897/50‑1 (MQ=255)
    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                          <  2:4289659/50‑1 (MQ=255)
     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:5010529/50‑1 (MQ=255)
      cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        <  2:1656838/50‑1 (MQ=255)
        tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      <  2:4159656/50‑1 (MQ=255)
        tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      <  1:24578/50‑1 (MQ=255)
        tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      <  1:324574/50‑1 (MQ=255)
          tCGCCTGCGCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  2:139591/49‑1 (MQ=38)
          tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  1:583261/49‑1 (MQ=255)
           cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  2:4879166/49‑1 (MQ=255)
           cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  2:2309615/49‑1 (MQ=255)
           cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                    <  1:2046365/49‑1 (MQ=255)
            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                  <  1:846244/50‑1 (MQ=255)
            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  2:288173/1‑49 (MQ=255)
            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   <  1:2262786/49‑1 (MQ=255)
            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  2:5128854/1‑49 (MQ=255)
             ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                 <  2:4452480/50‑1 (MQ=255)
             ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                 <  2:2110790/50‑1 (MQ=255)
              cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                <  1:4030882/50‑1 (MQ=255)
              cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  2:5124638/1‑47 (MQ=255)
               tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                >  2:1079212/1‑49 (MQ=255)
               tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                >  1:5325860/1‑49 (MQ=255)
                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                               <  1:3134177/48‑1 (MQ=255)
                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                               <  1:3637635/48‑1 (MQ=255)
                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                               <  2:2692034/48‑1 (MQ=255)
                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                              <  2:2845948/49‑1 (MQ=255)
                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             >  1:2877800/1‑50 (MQ=255)
                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             <  2:2490934/50‑1 (MQ=255)
                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             <  2:4792336/50‑1 (MQ=255)
                  aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  2:4129818/50‑1 (MQ=255)
                  aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  1:3330716/50‑1 (MQ=255)
                  aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  2:1527844/50‑1 (MQ=255)
                   ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                           <  1:404214/50‑1 (MQ=255)
                   ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                           >  2:5496137/1‑50 (MQ=255)
                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          <  1:795600/50‑1 (MQ=255)
                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          <  2:4371840/50‑1 (MQ=255)
                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          <  2:4863887/50‑1 (MQ=255)
                     ttCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         >  1:3335822/1‑50 (MQ=255)
                     ttCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         >  1:3731117/1‑50 (MQ=255)
                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  2:4750014/48‑1 (MQ=255)
                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  1:1767278/48‑1 (MQ=255)
                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  2:3587344/48‑1 (MQ=255)
                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  2:4198313/49‑1 (MQ=255)
                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  1:4905156/49‑1 (MQ=255)
                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  2:2563661/49‑1 (MQ=255)
                        ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      >  2:4967343/1‑50 (MQ=255)
                        ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      <  2:5512046/50‑1 (MQ=255)
                        ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      <  2:5587616/50‑1 (MQ=255)
                         gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCta                     <  2:657013/50‑2 (MQ=255)
                         gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     <  1:267604/50‑1 (MQ=255)
                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    >  2:3732004/1‑50 (MQ=255)
                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    <  2:3058422/50‑1 (MQ=255)
                           aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:2306020/1‑50 (MQ=255)
                           aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:5151079/1‑50 (MQ=255)
                            tCAGATGCGGTAATCAGTGACTTTGAAACCCAGAAGAATCCCTGCTCGt                   <  2:3148026/49‑1 (MQ=255)
                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  <  1:683137/50‑1 (MQ=255)
                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  <  1:4881371/50‑1 (MQ=255)
                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  2:2419276/1‑49 (MQ=255)
                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   <  1:1378246/49‑1 (MQ=255)
                             cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:1192349/1‑49 (MQ=255)
                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaa                <  1:5281709/50‑1 (MQ=255)
                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaa                <  2:3792131/50‑1 (MQ=255)
                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 <  2:5085033/49‑1 (MQ=255)
                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 <  1:1970906/49‑1 (MQ=255)
                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  2:2034625/1‑49 (MQ=255)
                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:3713037/1‑49 (MQ=255)
                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:1273882/50‑1 (MQ=255)
                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              <  2:411275/50‑1 (MQ=255)
                                 tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaaga             <  1:2676680/50‑1 (MQ=255)
                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa            <  2:4273006/49‑1 (MQ=255)
                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa            <  2:472612/49‑1 (MQ=255)
                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  2:1975570/1‑49 (MQ=255)
                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  2:3438168/1‑49 (MQ=255)
                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCg         <  2:1285283/50‑1 (MQ=255)
                                      tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa        >  1:4195035/1‑50 (MQ=255)
                                      tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa        >  2:3993562/1‑50 (MQ=255)
                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:1193608/49‑1 (MQ=255)
                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:1570554/49‑1 (MQ=255)
                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  2:3219058/49‑1 (MQ=255)
                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:1938393/49‑1 (MQ=255)
                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:1677845/1‑50 (MQ=255)
                                         tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  2:3243517/1‑49 (MQ=255)
                                          cAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  2:1546605/1‑48 (MQ=255)
                                          cAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:1819900/1‑48 (MQ=255)
                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCa    <  2:1721005/49‑1 (MQ=255)
                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCa    <  2:2412653/49‑1 (MQ=255)
                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   >  2:2200801/1‑50 (MQ=255)
                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   >  2:374325/1‑50 (MQ=255)
                                            gTGACGTTGAAGCCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  2:2555492/50‑1 (MQ=255)
                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  2:5739267/50‑1 (MQ=255)
                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  2:2633362/50‑1 (MQ=255)
                                               |                                              
GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑