Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,270,811 C→T V347M (GTG→ATG)  chaA ← Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,8110CT100.0% 601.5 / NA 148V347M (GTG→ATG) chaANa(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (79/69);  total (79/69)

Only 100 of 148 total aligned reads displayed.

CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857
                                               |                                              
catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:4016702/1‑50 (MQ=255)
catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  2:3954205/1‑50 (MQ=255)
 atcaAATAGGCGGCAAACAGTGCCAGATGGGCTGCGCCATTGAGCATGt                                              >  2:215870/1‑49 (MQ=38)
 atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                                             <  1:189312/50‑1 (MQ=255)
 atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              <  2:3284902/49‑1 (MQ=255)
  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  2:2971571/1‑48 (MQ=255)
  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:4315367/50‑1 (MQ=255)
   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  2:5511432/1‑50 (MQ=255)
    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                          <  2:2042381/50‑1 (MQ=255)
     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         >  1:5622242/1‑50 (MQ=255)
     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         >  1:2210184/1‑50 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  1:1698902/1‑50 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:5341284/50‑1 (MQ=255)
       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  1:4814073/50‑1 (MQ=255)
       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  2:4350515/50‑1 (MQ=255)
       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       >  2:2005393/1‑50 (MQ=255)
        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  2:5546297/1‑50 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      <  2:4128330/49‑1 (MQ=255)
         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  2:1226202/50‑1 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:1711285/50‑1 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    >  2:4946470/1‑50 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:5075668/50‑1 (MQ=255)
            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  >  2:1720394/1‑50 (MQ=255)
            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  >  2:3702231/1‑50 (MQ=255)
             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 >  1:2494187/1‑50 (MQ=255)
             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 >  1:488076/1‑50 (MQ=255)
             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 <  1:686068/50‑1 (MQ=255)
             gcAAACAGTGACAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 <  2:2873679/50‑1 (MQ=255)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:4176657/50‑1 (MQ=255)
              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:3567771/1‑50 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:1276013/1‑49 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:3377938/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:133347/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                              <  2:4270066/50‑1 (MQ=255)
                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:424521/1‑47 (MQ=255)
                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  2:2901185/50‑1 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                            <  1:3778991/50‑1 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                            <  2:1682325/50‑1 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  2:2383707/1‑49 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  2:462285/1‑49 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:5221951/1‑48 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:2427249/1‑48 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  2:3052638/1‑48 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                           <  1:596082/50‑1 (MQ=255)
                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:103652/1‑48 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:4927359/1‑47 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:3415277/1‑47 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                       <  2:1574285/50‑1 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:4602928/1‑46 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:206034/1‑46 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:2603077/1‑46 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:3107650/1‑46 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:3138151/50‑1 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  2:3215383/1‑49 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  2:4110894/50‑1 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:3935871/50‑1 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGGGGAGAAGGatat                     <  2:3450621/50‑1 (MQ=255)
                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   <  2:1782213/50‑1 (MQ=255)
                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   <  1:3885690/50‑1 (MQ=255)
                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  1:3002886/50‑1 (MQ=255)
                             gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:2435969/1‑50 (MQ=255)
                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 <  2:4104672/49‑1 (MQ=255)
                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 <  1:1004971/49‑1 (MQ=255)
                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 <  1:4157530/49‑1 (MQ=255)
                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc                <  2:4824104/50‑1 (MQ=255)
                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               <  2:3314217/50‑1 (MQ=255)
                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  2:3898977/1‑50 (MQ=255)
                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               <  2:803360/50‑1 (MQ=255)
                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  2:1106843/1‑50 (MQ=255)
                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:4665519/1‑49 (MQ=255)
                                  gcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            >  2:2330473/1‑50 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  1:4565785/50‑1 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  2:1316608/1‑50 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  2:1182584/1‑50 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  2:3766956/50‑1 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         <  1:2413171/50‑1 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:1512628/1‑50 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  2:3281195/1‑50 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  2:1765722/1‑49 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  2:1206449/1‑49 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:890126/1‑49 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:3264961/50‑1 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:5340778/50‑1 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  2:1066026/50‑1 (MQ=255)
                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:5623976/50‑1 (MQ=255)
                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  2:3077501/50‑1 (MQ=255)
                                        ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa      <  2:196979/50‑1 (MQ=255)
                                         tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  2:65937/1‑48 (MQ=255)
                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     <  1:788640/49‑1 (MQ=255)
                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:2940637/1‑47 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  1:1932608/1‑48 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  2:2605575/1‑48 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  2:269580/50‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  2:3458519/50‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  2:1866617/50‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:1980627/49‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:1428361/49‑1 (MQ=255)
                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  2:4390793/1‑50 (MQ=255)
                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  1:4899435/50‑1 (MQ=255)
                                            gCATGTTAGTACGTCCGGCGGAGAAGGAGATATGGCACAGCACTAAagag  >  2:2324851/1‑50 (MQ=255)
                                               |                                              
CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑