BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	459.9 / NA	118	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (71/47); total (71/47)

Only 100 of 136 total aligned reads displayed.

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074

aaTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcct                                              >  1:4299934/1‑50 (MQ=255)

aaTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcct                                              <  2:2893472/50‑1 (MQ=255)

 aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             >  1:496806/1‑50 (MQ=255)

 aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             >  1:2300683/1‑50 (MQ=255)

  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:5667133/1‑50 (MQ=255)

  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:5757577/1‑50 (MQ=255)

  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:1176826/50‑1 (MQ=255)

  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:798231/50‑1 (MQ=255)

  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  2:2216879/50‑1 (MQ=255)

   aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:1228149/1‑49 (MQ=255)

   aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:3848194/1‑49 (MQ=255)

    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  2:218383/49‑1 (MQ=255)

    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:2553382/1‑48 (MQ=255)

    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:1647303/1‑48 (MQ=255)

    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCa                                          <  1:1760133/50‑1 (MQ=255)

    gTCCAGTATGTTCCTTGCGATAAACGTGCATGGCATGCGGGGGcctctc                                           <  2:1256354/49‑1 (MQ=255)

     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  2:2591531/1‑50 (MQ=255)

     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         <  1:789564/50‑1 (MQ=255)

      ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  2:4771018/1‑49 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGt                                        <  2:588602/49‑1 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  2:3687567/1‑50 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       <  1:4607397/50‑1 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  2:5654122/1‑50 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       <  1:186433/50‑1 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  2:1559114/1‑50 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  1:2579984/1‑50 (MQ=255)

          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:2217014/1‑48 (MQ=255)

           aTGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   >  2:2054404/1‑50 (MQ=255)

           aTGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   >  1:5701306/1‑50 (MQ=255)

            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   <  2:4537778/49‑1 (MQ=255)

             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:74275/1‑50 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                >  2:2937012/1‑50 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                <  2:3101763/50‑1 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                <  2:3632971/50‑1 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                <  1:2056768/50‑1 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                >  1:3315626/1‑50 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 <  1:2893101/49‑1 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 <  2:5062072/49‑1 (MQ=255)

               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCTTCTCAGTATCAGGGgc                               >  2:1410829/1‑50 (MQ=255)

               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  1:4438632/1‑50 (MQ=255)

               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  2:5737823/1‑50 (MQ=255)

                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              >  1:233427/1‑50 (MQ=255)

                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:1507448/1‑50 (MQ=255)

                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  1:4455289/50‑1 (MQ=255)

                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:1983733/1‑49 (MQ=255)

                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  2:1336100/1‑49 (MQ=255)

                   ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            >  2:1927052/1‑49 (MQ=255)

                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                          >  2:4506546/1‑50 (MQ=255)

                     cGATCAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:4028085/1‑50 (MQ=39)

                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:291755/1‑50 (MQ=255)

                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  2:1030334/1‑50 (MQ=255)

                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:3098951/1‑50 (MQ=255)

                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:3422020/1‑50 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        >  2:3274909/1‑50 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        >  2:5711389/1‑50 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        >  1:4804322/1‑50 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        >  1:4299612/1‑50 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        <  1:4571544/50‑1 (MQ=255)

                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  1:2726529/1‑50 (MQ=255)

                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  2:3155231/1‑50 (MQ=255)

                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      <  2:2207535/50‑1 (MQ=255)

                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      <  1:621456/50‑1 (MQ=255)

                         aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGTGGCGCGAACGCTg                     >  1:5445716/1‑50 (MQ=38)

                         aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:5536765/1‑50 (MQ=255)

                         aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  2:3700303/1‑50 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     <  2:579993/49‑1 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  2:547059/1‑49 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  2:3885733/1‑49 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  1:5429988/50‑1 (MQ=255)

                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  1:5358554/1‑50 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:1887195/1‑50 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  2:2807968/50‑1 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:2161033/1‑50 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGACGCGAACGCTGCAAt                 >  1:442517/1‑50 (MQ=255)

                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  2:4549068/50‑1 (MQ=255)

                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  2:4133208/50‑1 (MQ=255)

                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  1:2805504/50‑1 (MQ=255)

                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:640773/1‑50 (MQ=255)

                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:5607510/1‑50 (MQ=255)

                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:4551473/1‑50 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  1:5401450/1‑50 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             <  2:1554723/50‑1 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  2:5229261/1‑50 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             <  2:861738/50‑1 (MQ=255)

                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt           <  2:4318506/49‑1 (MQ=255)

                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt           <  2:1112003/49‑1 (MQ=255)

                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         <  1:3973877/50‑1 (MQ=255)

                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         <  2:901176/50‑1 (MQ=255)

                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  2:4636274/1‑49 (MQ=255)

                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:4249617/1‑49 (MQ=255)

                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt        <  2:3617561/50‑1 (MQ=255)

                                       tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa       <  1:4046166/50‑1 (MQ=255)

                                       tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa       <  2:2417738/50‑1 (MQ=255)

                                        gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      >  2:4357139/1‑50 (MQ=255)

                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  1:3507914/1‑50 (MQ=255)

                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  1:952385/1‑50 (MQ=255)

                                           ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   <  1:5589921/50‑1 (MQ=255)

                                           ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   <  1:1730172/50‑1 (MQ=255)

                                           ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   >  1:1319188/1‑50 (MQ=255)

                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:2923517/50‑1 (MQ=255)

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`