| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 214,885 | C→T | T18I (ACC→ATC) | arfB → | peptidyl‑tRNA hydrolase, ribosome rescue factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 214,885 | 0 | C | T | 94.8% | 500.3 / 11.6 | 136 | T18I (ACC→ATC) | arfB | peptidyl‑tRNA hydrolase, ribosome rescue factor |
| Reads supporting (aligned to +/- strand): ref base C (3/4); new base T (55/73); total (58/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
TTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC > NC_000913/214837‑214931 | ttGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCAcc < 2:4483434/50‑1 (MQ=255) tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg > 1:1725192/1‑50 (MQ=255) tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg > 1:3141496/1‑50 (MQ=255) gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg < 2:4637656/49‑1 (MQ=255) gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg < 2:64750/49‑1 (MQ=255) gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg < 1:2646052/49‑1 (MQ=255) gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc > 2:4001290/1‑50 (MQ=255) tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc > 1:2790090/1‑49 (MQ=255) gATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa > 2:4010238/1‑50 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa < 2:1197093/49‑1 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt > 2:1671729/1‑50 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt < 2:5502937/50‑1 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt > 2:5153985/1‑50 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt > 1:4998777/1‑50 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt > 2:2869411/1‑50 (MQ=255) tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt < 1:2725551/50‑1 (MQ=255) tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt < 1:4612995/50‑1 (MQ=255) tttCCCGACATGTTGCTATTCCCGATGGTGAGATTGAGATCATCGCCAtt < 2:1902455/50‑1 (MQ=255) tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc < 1:5133178/49‑1 (MQ=255) tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc < 2:4722335/49‑1 (MQ=255) tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc < 1:3792822/49‑1 (MQ=255) tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTc < 2:1115090/49‑1 (MQ=255) cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg < 2:586291/49‑1 (MQ=255) cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg < 2:2827291/49‑1 (MQ=255) cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt > 2:345533/1‑50 (MQ=255) cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt > 1:714889/1‑50 (MQ=255) ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg > 2:5727940/1‑50 (MQ=255) cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc < 2:942819/50‑1 (MQ=255) cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc > 2:3684312/1‑50 (MQ=255) cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc > 1:3685282/1‑50 (MQ=255) cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc < 1:2582143/50‑1 (MQ=255) cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc < 1:1470148/50‑1 (MQ=255) cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCAGCGCCATTCGTgc < 2:3997678/50‑1 (MQ=255) gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg < 2:4864541/50‑1 (MQ=255) gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg > 1:2918604/1‑50 (MQ=255) gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg > 2:1423684/1‑50 (MQ=255) gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg > 1:2014117/1‑50 (MQ=255) gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg < 2:1078852/50‑1 (MQ=255) cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCa < 1:3272825/50‑1 (MQ=255) aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg > 1:3720953/1‑50 (MQ=255) aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg < 1:5411879/50‑1 (MQ=255) tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg < 2:1348396/50‑1 (MQ=255) tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg > 2:1374327/1‑50 (MQ=255) gTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg > 2:5709977/1‑50 (MQ=255) gTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg > 1:4424852/1‑50 (MQ=255) ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc > 2:3561821/1‑50 (MQ=255) ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc > 1:1942337/1‑50 (MQ=255) ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc > 1:3328994/1‑50 (MQ=255) tGCTATTCCCGATTTTTAGCTTTAGATCATCTCCATTCGTGCGCAGGgcg > 2:4937527/1‑50 (MQ=255) aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg > 2:866155/1‑50 (MQ=255) aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg > 2:3699074/1‑50 (MQ=255) aTTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCgcggg > 2:3613240/1‑50 (MQ=255) ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc < 1:2161380/50‑1 (MQ=255) ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc < 2:2242514/50‑1 (MQ=255) ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg < 1:4445596/48‑1 (MQ=255) ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg < 2:1358096/48‑1 (MQ=255) cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg > 2:1265495/1‑50 (MQ=255) cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcg < 1:4902431/49‑1 (MQ=255) ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg < 2:5345302/49‑1 (MQ=255) ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg < 2:2758137/49‑1 (MQ=255) cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCgggcgggc < 2:2243518/50‑1 (MQ=255) cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCgggcgggc < 1:1011474/50‑1 (MQ=255) gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg > 2:2428474/1‑48 (MQ=255) gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgca < 2:897233/50‑1 (MQ=255) aTGGTGAGCTTGAGCTCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag > 2:87569/1‑50 (MQ=39) aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag > 2:2175652/1‑50 (MQ=255) tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag > 1:411485/1‑49 (MQ=255) gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca < 1:3526941/49‑1 (MQ=255) gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca < 1:1373234/49‑1 (MQ=255) gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca < 2:2684465/49‑1 (MQ=255) gTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca < 2:2208210/49‑1 (MQ=255) tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg < 2:2606282/50‑1 (MQ=255) tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg < 2:1152313/50‑1 (MQ=255) tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg < 1:5367975/50‑1 (MQ=255) tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg < 2:5409664/50‑1 (MQ=255) gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt > 1:4319116/1‑50 (MQ=255) aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt < 1:2752149/50‑1 (MQ=255) aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt < 1:5247725/49‑1 (MQ=255) aGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt < 2:4382035/49‑1 (MQ=255) gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt > 1:960519/1‑49 (MQ=255) gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt > 1:3039983/1‑49 (MQ=255) gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta < 2:2781279/50‑1 (MQ=255) gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta < 2:5354705/50‑1 (MQ=255) gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta < 2:2405785/50‑1 (MQ=255) gCTTGAGATCATCGCCATTCGAGCGCAGGGCGCGGGCGGGCAGCATGtt > 1:3203738/1‑49 (MQ=38) cTTGGGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa < 2:2435678/50‑1 (MQ=38) cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa < 1:561561/50‑1 (MQ=255) cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa > 1:557015/1‑50 (MQ=255) cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa < 2:1490262/50‑1 (MQ=255) ttGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa > 1:4931064/1‑49 (MQ=255) gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata < 1:5199493/49‑1 (MQ=255) gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata < 2:2586642/49‑1 (MQ=255) gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat < 2:785474/48‑1 (MQ=255) gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat > 1:3374655/1‑48 (MQ=255) gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat < 2:4703761/48‑1 (MQ=255) agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg < 1:2211203/50‑1 (MQ=255) gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa < 2:863780/50‑1 (MQ=255) atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc > 1:382322/1‑50 (MQ=255) atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc > 1:650984/1‑50 (MQ=255) atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc < 2:2194522/50‑1 (MQ=255) | TTGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC > NC_000913/214837‑214931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |