Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,091,305 A→G S58G (AGC→GGC)  rsmE → 16S rRNA m(3)U1498 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,091,3050AG100.0% 688.7 / NA 171S58G (AGC→GGC) rsmE16S rRNA m(3)U1498 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (86/85);  total (86/85)

Only 100 of 171 total aligned reads displayed.

GTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGA  >  NC_000913/3091259‑3091351
                                              |                                              
gTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGc                                              >  2:4398850/1‑49 (MQ=255)
 tttGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCa                                             >  1:5771350/1‑49 (MQ=255)
   tGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaa                                           <  1:1086546/49‑1 (MQ=255)
   tGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaa                                           >  1:4885520/1‑49 (MQ=255)
   tGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaa                                           <  1:2680833/49‑1 (MQ=255)
    gACGGTAGTAACCAGGTCTTTGACGCAGAAATTACCAGCGCCGGCaaaa                                          <  2:2006525/49‑1 (MQ=38)
    gACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaa                                          >  1:1264536/1‑49 (MQ=255)
     aCGGTAGCAACCAGGTCTTTGACGCCGAAATTTCCAGCGCCGGCaaaaa                                         <  2:1546178/49‑1 (MQ=39)
     aCGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaaa                                        <  2:1332552/50‑1 (MQ=255)
     aCGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaa                                         >  1:4254971/1‑49 (MQ=255)
     aCGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaa                                         >  2:5766164/1‑49 (MQ=255)
     aCGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaa                                         <  1:1290061/49‑1 (MQ=255)
      cGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaaaa                                       <  2:5785143/50‑1 (MQ=255)
      cGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaaaa                                       <  2:3386014/50‑1 (MQ=255)
       ggTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAg                                      <  2:1747545/50‑1 (MQ=255)
       ggTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAg                                      >  1:482517/1‑50 (MQ=255)
        gTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGc                                     <  2:260953/50‑1 (MQ=255)
         tAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCg                                    >  1:33278/1‑50 (MQ=255)
          aGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGt                                   >  1:4758217/1‑50 (MQ=255)
            cAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTgg                                 <  1:2100632/50‑1 (MQ=255)
            cAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTgg                                 <  1:5092664/50‑1 (MQ=255)
             aaCCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTgg                                 >  1:365819/1‑49 (MQ=255)
             aaCCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTgg                                 >  2:3886726/1‑49 (MQ=255)
             aaCCAGGTCTTTGAAGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTgg                                 >  2:3153151/1‑49 (MQ=39)
              aCCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTgg                                 >  1:527378/1‑48 (MQ=255)
              aCCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGaa                               <  1:2730292/50‑1 (MQ=255)
              aCCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGaa                               <  2:2672257/50‑1 (MQ=255)
               ccAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAg                              >  2:2312090/1‑50 (MQ=255)
               ccAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAg                              <  1:5431881/50‑1 (MQ=255)
               ccAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAg                              <  1:1552251/50‑1 (MQ=255)
               ccAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAg                              <  2:4285840/50‑1 (MQ=255)
                cAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                             >  1:2966281/1‑50 (MQ=255)
                cAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                             <  1:2340713/50‑1 (MQ=255)
                cAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                             <  1:2214411/50‑1 (MQ=255)
                cAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                             >  2:4167457/1‑50 (MQ=255)
                cAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                             >  1:790252/1‑50 (MQ=255)
                 aGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                             >  2:2093321/1‑49 (MQ=255)
                 aGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                             >  1:2401782/1‑49 (MQ=255)
                 aGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTg                            <  1:1564615/50‑1 (MQ=255)
                  ggTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTg                            >  1:392520/1‑49 (MQ=255)
                  ggTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTg                            >  1:1196602/1‑49 (MQ=255)
                  ggTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTg                            >  1:695237/1‑49 (MQ=255)
                  ggTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGa                           <  1:5598071/50‑1 (MQ=255)
                   gTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGaa                          <  2:1743605/50‑1 (MQ=255)
                    tCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAg                         <  2:348233/50‑1 (MQ=255)
                     cTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAgg                        >  1:4002088/1‑50 (MQ=255)
                     cTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAgg                        >  1:245917/1‑50 (MQ=255)
                      tttGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       >  2:1146261/1‑50 (MQ=255)
                      tttGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       >  2:5365346/1‑50 (MQ=255)
                      tttGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       >  2:3960032/1‑50 (MQ=255)
                      tttGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       >  2:968963/1‑50 (MQ=255)
                      tttGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       >  1:1999072/1‑50 (MQ=255)
                       ttGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTg                      >  2:1650698/1‑50 (MQ=255)
                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAgg                        <  2:4535612/47‑1 (MQ=255)
                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAgg                        <  1:5286113/47‑1 (MQ=255)
                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       <  1:2921014/48‑1 (MQ=255)
                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       <  2:2549538/48‑1 (MQ=255)
                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       <  2:1304875/48‑1 (MQ=255)
                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                       <  1:5548423/48‑1 (MQ=255)
                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTg                      <  1:4006233/49‑1 (MQ=255)
                         gACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGc                     <  1:951106/49‑1 (MQ=255)
                         gACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCt                    >  2:42441/1‑50 (MQ=255)
                          aCGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTg                   >  1:1788801/1‑50 (MQ=255)
                          aCGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTg                   <  1:5488211/50‑1 (MQ=255)
                            gCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTgg                  >  1:2081575/1‑49 (MQ=255)
                            gCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGa                 <  2:5244134/50‑1 (MQ=255)
                             ccGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGaa                <  1:66273/50‑1 (MQ=255)
                              cGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAg               <  1:1472566/50‑1 (MQ=255)
                              cGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAg               >  1:2132605/1‑50 (MQ=255)
                               gAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAgg              >  1:4924662/1‑50 (MQ=255)
                               gAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAgg              <  1:1048373/50‑1 (MQ=255)
                               gAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGGGAAGGTGCTGGAAgg              >  2:3997034/1‑50 (MQ=38)
                                aaaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             <  1:625245/50‑1 (MQ=255)
                                aaaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  2:278506/1‑50 (MQ=255)
                                aaaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  1:4991465/1‑50 (MQ=255)
                                 aaTTACCAGCGCCGGCAAAAAAAGCGTTGAAGTGAAGGTGCTGGAAGGc             >  1:2857148/1‑49 (MQ=39)
                                 aaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGcc            <  1:4530707/50‑1 (MQ=255)
                                 aaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  2:5809442/1‑49 (MQ=255)
                                 aaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  1:5023960/1‑49 (MQ=255)
                                  aTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  2:2824484/1‑48 (MQ=255)
                                   ttACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  2:3536554/1‑47 (MQ=255)
                                     aCCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  1:31760/1‑45 (MQ=255)
                                     aCCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  1:2008225/1‑45 (MQ=255)
                                     aCCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGa         <  1:3014289/49‑1 (MQ=255)
                                     aCCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGa         <  1:2395527/49‑1 (MQ=255)
                                      ccAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc             >  2:1937108/1‑44 (MQ=255)
                                      ccAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGAt        <  2:2899061/49‑1 (MQ=255)
                                      ccAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGAt        <  2:3558361/49‑1 (MQ=255)
                                      ccAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATc       <  2:434728/50‑1 (MQ=255)
                                      ccAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATc       <  1:1908068/50‑1 (MQ=255)
                                       cAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcg      <  1:3504185/50‑1 (MQ=255)
                                       cAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcg      <  1:5631948/50‑1 (MQ=255)
                                       cAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATc       >  2:4847243/1‑49 (MQ=255)
                                       cAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATc       >  2:3109475/1‑49 (MQ=255)
                                          cgcCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcgacg   <  2:2439561/50‑1 (MQ=255)
                                           gcCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcgacga  >  1:4420464/1‑50 (MQ=255)
                                           gcCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcgacga  >  2:5094176/1‑50 (MQ=255)
                                           gcCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcgacga  >  2:5151650/1‑50 (MQ=255)
                                           gcCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcgacga  >  1:4836625/1‑50 (MQ=255)
                                           gcCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATcgacga  >  2:5563274/1‑50 (MQ=255)
                                              |                                              
GTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGA  >  NC_000913/3091259‑3091351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑