| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,213,803 | T→C | S253P (TCT→CCT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,213,803 | 0 | T | C | 94.0% | 607.2 / 15.9 | 168 | S253P (TCT→CCT) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base C (80/78); total (82/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
Only 100 of 168 total aligned reads displayed.
CGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACTA > NC_000913/3213756‑3213849 | cGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt < 1:4281265/50‑1 (MQ=255) gAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt > 1:484037/1‑49 (MQ=255) aaaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTg > 2:4951406/1‑49 (MQ=255) aaaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGa < 2:5568086/50‑1 (MQ=255) aaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGaa < 1:3529524/50‑1 (MQ=255) aGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAg < 1:1156426/50‑1 (MQ=255) ggTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGt < 2:4568074/50‑1 (MQ=255) ggTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGt < 1:754691/50‑1 (MQ=255) ggTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGt < 1:99295/50‑1 (MQ=255) gTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTa < 1:1015138/50‑1 (MQ=255) gTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTa < 1:5627251/50‑1 (MQ=255) gTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTa > 1:813555/1‑50 (MQ=255) tCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAt < 1:4420894/50‑1 (MQ=255) tCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAt > 2:4974126/1‑50 (MQ=255) cGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt > 1:3634930/1‑50 (MQ=255) cGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt > 2:4588278/1‑50 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAttg < 1:5469653/50‑2 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt < 2:699862/49‑1 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt < 1:4648843/49‑1 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt > 2:4761452/1‑49 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTc < 1:3555911/50‑1 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTc < 2:3227156/50‑1 (MQ=255) gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTc < 2:1920343/50‑1 (MQ=255) cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCa < 1:605908/50‑1 (MQ=255) cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTc > 1:594138/1‑49 (MQ=255) cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa < 1:1784868/50‑1 (MQ=255) cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa < 1:3360224/50‑1 (MQ=255) aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaa < 2:4222778/50‑1 (MQ=255) aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa > 1:3896818/1‑49 (MQ=255) aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa > 2:901963/1‑49 (MQ=255) aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa > 2:3106116/1‑49 (MQ=255) aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCa > 1:345818/1‑49 (MQ=255) gTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaaa > 1:1871455/1‑50 (MQ=255) tCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAAc < 1:1338794/50‑1 (MQ=255) tCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAAc < 1:4143183/50‑1 (MQ=255) tCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAAc < 1:5521906/50‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACa < 2:2548945/49‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACa < 1:845286/49‑1 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAg > 1:5354237/1‑50 (MQ=255) aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAg < 2:57151/50‑1 (MQ=255) gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt < 2:3967165/50‑1 (MQ=255) gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt < 1:2839814/50‑1 (MQ=255) gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt > 1:141129/1‑50 (MQ=255) cTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTc < 2:3737080/50‑1 (MQ=255) tACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc > 2:2208865/1‑50 (MQ=255) tACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc > 1:4023005/1‑50 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc < 1:2892351/48‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCg < 1:1448681/49‑1 (MQ=255) ccGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCg < 1:1404850/49‑1 (MQ=255) cGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGcc > 2:2845964/1‑50 (MQ=255) cGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGcc < 2:4915246/50‑1 (MQ=255) cGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGcc > 2:3572646/1‑50 (MQ=255) gCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCt < 1:5478945/50‑1 (MQ=255) gCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCt < 1:5307361/50‑1 (MQ=255) gCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTACCGCCt < 2:3833061/50‑1 (MQ=39) cTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTg < 2:4503522/50‑1 (MQ=255) cTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTg > 2:713112/1‑50 (MQ=255) tCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTgg > 2:5386601/1‑50 (MQ=255) tCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTgg > 2:1729178/1‑50 (MQ=255) cAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGt > 1:5706528/1‑50 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGt < 2:5266368/49‑1 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGt < 1:3322550/49‑1 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTg < 1:5046238/50‑1 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTg > 1:3482419/1‑50 (MQ=255) aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTg > 2:2700992/1‑50 (MQ=255) ggAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGc < 2:5470482/50‑1 (MQ=255) ggAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGc > 1:4471505/1‑50 (MQ=255) gAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGcc > 1:2790230/1‑50 (MQ=255) gAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGcc > 2:3134411/1‑50 (MQ=255) aaGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCg < 2:4258689/50‑1 (MQ=255) aaGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGcc > 1:2883201/1‑49 (MQ=255) agaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 1:1542568/1‑50 (MQ=255) gaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 1:2523032/1‑49 (MQ=255) gaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 1:2783253/1‑49 (MQ=255) gaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 1:2246525/1‑49 (MQ=255) aTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 2:3403893/1‑48 (MQ=255) aTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg > 2:1538352/1‑48 (MQ=255) aTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCa < 2:1216742/50‑1 (MQ=255) tCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAg < 2:5264716/50‑1 (MQ=255) ccTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGt > 2:3019932/1‑50 (MQ=255) cTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGtt > 2:195758/1‑50 (MQ=255) cTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGt < 1:3298825/49‑1 (MQ=255) cTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGtt > 1:2536547/1‑50 (MQ=255) cTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGtt < 1:1188291/50‑1 (MQ=255) cTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGtt > 1:2494303/1‑50 (MQ=255) cTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGtt > 2:1480885/1‑50 (MQ=255) tGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt > 1:4109388/1‑50 (MQ=255) aaaCTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTg < 1:1504848/49‑1 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCTAAGCAGTTTGa > 1:1100520/1‑50 (MQ=39) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt < 1:2988718/48‑1 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTg < 1:1867631/49‑1 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa > 1:1016655/1‑50 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa > 2:3467286/1‑50 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa > 2:1901890/1‑50 (MQ=255) aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa > 1:4724884/1‑50 (MQ=255) aaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGAc > 2:16686/1‑50 (MQ=255) aCTGCCTGAAGTATTCAATCAGTTCCGTCTGGTGCCGAAGCAGTTTGTCt < 2:3860482/50‑1 (MQ=37) aCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGAc > 2:2262458/1‑49 (MQ=255) aCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACt < 2:805056/50‑1 (MQ=255) cTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACTa > 2:3046832/1‑50 (MQ=255) | CGAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACTA > NC_000913/3213756‑3213849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |