Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,477,150 C→T K90K (AAG→AAA)  fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,1500CT100.0% 996.6 / NA 243K90K (AAG→AAA) fkpApeptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (109/134);  total (109/134)

Only 100 of 247 total aligned reads displayed.

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196
                                              |                                              
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  2:5417820/49‑1 (MQ=255)
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:2417272/49‑1 (MQ=255)
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  1:3644817/1‑50 (MQ=255)
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  2:352475/1‑50 (MQ=255)
cGAATGCTTGTAGAGTCTGGTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  2:4017780/49‑1 (MQ=39)
 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  1:4918460/50‑1 (MQ=255)
 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  2:4542716/50‑1 (MQ=255)
  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                           <  2:2319639/50‑1 (MQ=255)
  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                           <  1:832487/50‑1 (MQ=255)
   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  2:1719809/1‑48 (MQ=255)
   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  2:1425514/1‑48 (MQ=255)
   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  2:2034566/50‑1 (MQ=255)
    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         <  1:2131555/50‑1 (MQ=255)
    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         <  2:4585779/50‑1 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        >  2:5710378/1‑49 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:870640/49‑1 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:537816/49‑1 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       <  1:4809645/50‑1 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       <  1:2973088/50‑1 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       <  2:2374586/50‑1 (MQ=255)
       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  2:382271/1‑49 (MQ=255)
       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:1742963/1‑49 (MQ=255)
       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:4079801/1‑49 (MQ=255)
       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  2:2065426/1‑49 (MQ=255)
       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:4021294/1‑49 (MQ=255)
        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:116590/1‑50 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  2:438987/49‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    >  1:392373/1‑50 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  2:832963/49‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  2:1602074/1‑50 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:5355621/50‑1 (MQ=255)
           agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  1:1373143/1‑49 (MQ=255)
            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                 >  2:5774661/1‑50 (MQ=255)
             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  2:3090485/50‑1 (MQ=255)
             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:4685706/50‑1 (MQ=255)
             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                >  1:1292877/1‑50 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               <  2:127275/50‑1 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               <  2:5351071/50‑1 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:1008845/1‑50 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               <  1:5840940/50‑1 (MQ=255)
               tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                              <  2:3129278/50‑1 (MQ=255)
                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  2:1093756/1‑49 (MQ=255)
                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  2:1176622/1‑49 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  1:2537101/47‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  2:3242798/47‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  2:2815107/47‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  2:4417176/47‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  2:5532266/47‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:2886998/1‑50 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAGGCATCCTGa                           <  2:4716013/49‑1 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  2:4986634/1‑50 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                         <  2:4901420/49‑1 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                         <  2:2909284/49‑1 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:5625893/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        <  1:3573908/49‑1 (MQ=255)
                         ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    >  1:5579730/1‑50 (MQ=255)
                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:3203228/1‑50 (MQ=255)
                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  2:5584423/1‑49 (MQ=255)
                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:2343643/1‑49 (MQ=255)
                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  2:134053/1‑49 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  2:722759/44‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  2:3274454/44‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  2:1557599/46‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  2:1880939/48‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  2:4082794/48‑1 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:2234514/49‑1 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  2:5072636/49‑1 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:5165596/49‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  2:1043421/49‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  2:2263912/50‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  2:1154722/50‑1 (MQ=255)
                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             <  1:1470531/50‑1 (MQ=255)
                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  1:5310994/1‑50 (MQ=255)
                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  1:2493265/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAgtt           <  2:4741155/50‑3 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:588359/49‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:4751538/49‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:5086079/50‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:5027921/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:1579301/50‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:4590040/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:1967547/50‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:440147/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  2:2491846/50‑1 (MQ=255)
                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:2044104/1‑49 (MQ=255)
                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  2:4494331/1‑49 (MQ=255)
                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:1824962/1‑49 (MQ=255)
                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         >  2:3455976/1‑50 (MQ=255)
                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       <  2:2712250/50‑1 (MQ=255)
                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  2:366815/1‑50 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:3956561/1‑50 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:4127471/50‑1 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:3386336/1‑50 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  2:5388277/1‑50 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  2:664506/50‑1 (MQ=255)
                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     <  1:4510939/50‑1 (MQ=255)
                                         ttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     >  1:108881/1‑49 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:264105/49‑1 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   >  2:1336316/1‑50 (MQ=255)
                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   <  2:1951752/49‑1 (MQ=255)
                                              |                                              
CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑