| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,760 | A→G | N431S (AAC→AGC) | igaA → | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,760 | 0 | A | G | 85.8% | 530.5 / 67.2 | 183 | N431S (AAC→AGC) | igaA | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Reads supporting (aligned to +/- strand): ref base A (10/16); new base G (99/58); total (109/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 203 total aligned reads displayed.
AACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA > NC_000913/3527713‑3527804 | aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga > 2:2531315/1‑50 (MQ=255) aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAacga < 2:5692212/50‑1 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 2:5123977/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 1:1622374/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 2:2657960/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 1:2553420/50‑1 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 2:4733891/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 2:3780760/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg < 2:2137644/50‑1 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc < 1:5344366/50‑1 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc > 1:2217847/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGcc > 1:1988413/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGcc < 2:4111535/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 2:2333709/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:2496213/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:1464894/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 2:5768942/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGccc < 2:1791032/50‑1 (MQ=255) gtCACCTTTTTTTCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:5755310/49‑1 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:2031652/1‑50 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc < 1:533273/50‑1 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTc < 2:1398004/50‑1 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTc < 2:3962576/50‑1 (MQ=255) cTTTTTTGCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa < 2:1748926/50‑1 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa > 2:5623990/1‑50 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:3325857/1‑50 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:5601222/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 1:1074575/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 2:4501709/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 2:2995833/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt < 1:3808889/49‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTg > 1:5324135/1‑50 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg < 2:416160/49‑1 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 2:2059587/1‑50 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAACTACGCCCGCTCATTg < 1:2276363/49‑1 (MQ=255) ttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 2:1643791/1‑49 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 2:4352553/1‑50 (MQ=255) ccGTTTGACTGCTCGCAGATCCTCTGGAGCGACGCCCGCTCATTGCCAt > 2:5079094/1‑49 (MQ=38) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:4290267/1‑49 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt < 1:3185490/49‑1 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGAGACGCCCGCTCATTGCCAt > 2:1461203/1‑49 (MQ=38) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa > 1:2616140/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 2:4367529/50‑1 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 2:211927/50‑1 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:4906559/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 2:10299/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATCGCCATTAc < 2:61922/50‑1 (MQ=255) ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:3483907/1‑48 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:1888491/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:4589554/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAc > 2:1551511/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCa < 2:4135642/49‑1 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:1253820/1‑49 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:5384673/1‑49 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCCCATTGCCATTACCAg > 2:3545467/1‑49 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 1:4707814/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGa < 2:5554878/49‑1 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGa < 1:1939640/49‑1 (MQ=255) aCTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGa < 2:3189096/49‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:4345724/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 2:2394858/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:288182/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt > 2:2819881/1‑50 (MQ=255) cTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAAt > 1:3008637/1‑50 (MQ=255) tacTCGCAGTACATCTGGAGCGACGCCCTCTCATTGCCATTACCAGAATc > 2:4881476/3‑50 (MQ=255) gCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:4190575/1‑50 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATc < 2:710706/48‑1 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt < 2:1211553/49‑1 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt < 2:2726628/49‑1 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:2749042/1‑49 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:561848/1‑49 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:5810277/1‑49 (MQ=255) cGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa > 2:2202874/1‑50 (MQ=255) cGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa > 2:3300695/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc < 2:5473340/50‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:335430/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:3721291/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc < 1:4779062/50‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc < 1:5460266/50‑1 (MQ=255) gCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGa < 2:1867790/48‑1 (MQ=255) cAGATCATCTGGAGTGACGCCCGCTCATTGCCATTACCAGAATCTGAACt > 2:5567847/1‑50 (MQ=39) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:4976978/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:5167533/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg > 1:4000093/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg < 2:5075304/50‑1 (MQ=255) gATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg < 2:800072/50‑1 (MQ=255) atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt < 1:451136/50‑1 (MQ=255) atcatcTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt < 1:1571904/50‑1 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc < 2:1078943/50‑1 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc < 1:3219506/50‑1 (MQ=255) catcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc > 2:2859843/1‑49 (MQ=255) catcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc > 2:5232091/1‑49 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa < 1:4182998/50‑1 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca < 2:4037182/49‑1 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca < 1:2451303/49‑1 (MQ=255) tcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 2:43969/1‑49 (MQ=255) tcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 2:4276734/1‑49 (MQ=255) tcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:5236899/1‑49 (MQ=255) tGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:1863758/1‑49 (MQ=255) gAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca < 1:913950/47‑1 (MQ=255) | AACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA > NC_000913/3527713‑3527804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |