Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,093,223 A→G V72V (GTT→GTC)  rhaM ← L‑rhamnose mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,093,2230AG100.0% 489.0 / NA 121V72V (GTT→GTC) rhaML‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (78/43);  total (78/43)

Only 100 of 121 total aligned reads displayed.

GGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093177‑4093269
                                              |                                              
ggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACa                                             >  2:3544812/1‑50 (MQ=255)
 gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                            >  1:5464093/1‑50 (MQ=255)
  ttAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                            >  2:1753687/1‑49 (MQ=255)
    aGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCa                                          <  2:4561325/49‑1 (MQ=255)
     gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                        >  1:4137746/1‑50 (MQ=255)
     gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                        <  1:2891897/50‑1 (MQ=255)
     gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                        <  1:2375405/50‑1 (MQ=255)
      cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       <  2:4623218/50‑1 (MQ=255)
      cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       >  2:3571652/1‑50 (MQ=255)
      cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       <  2:2004987/50‑1 (MQ=255)
      cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       >  2:270247/1‑50 (MQ=255)
       gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                      >  1:4896202/1‑50 (MQ=255)
       gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                      >  1:2226517/1‑50 (MQ=255)
       gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                      >  1:825031/1‑50 (MQ=255)
        ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                     <  2:1788386/50‑1 (MQ=255)
        ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                     <  1:593842/50‑1 (MQ=255)
        ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                     <  2:5415544/50‑1 (MQ=255)
         gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    >  2:2318228/1‑50 (MQ=255)
         gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    >  2:2291753/1‑50 (MQ=255)
         gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    <  2:4843368/50‑1 (MQ=255)
         gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    >  2:5801202/1‑50 (MQ=255)
          cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  2:2939203/1‑50 (MQ=255)
          cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   <  1:5445255/50‑1 (MQ=255)
          cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  1:5103066/1‑50 (MQ=255)
           aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  2:775457/1‑49 (MQ=255)
           aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  <  2:921880/50‑1 (MQ=255)
           aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  <  1:3061698/50‑1 (MQ=255)
            tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  >  1:3711779/1‑49 (MQ=255)
             aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa                                >  2:4712596/1‑50 (MQ=255)
              aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc                               >  1:5754350/1‑50 (MQ=255)
               cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc                              >  2:857732/1‑50 (MQ=255)
               cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc                              <  2:5021626/50‑1 (MQ=255)
               cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc                              >  2:5233291/1‑50 (MQ=255)
               cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc                              <  1:4458389/50‑1 (MQ=255)
               cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc                              >  2:3634956/1‑50 (MQ=255)
               cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc                              >  2:5348269/1‑50 (MQ=255)
                aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCCACCg                             <  1:4793749/50‑1 (MQ=255)
                aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  2:5094478/1‑50 (MQ=255)
                 tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:3939750/1‑49 (MQ=255)
                 tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:2461913/1‑49 (MQ=255)
                 tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:3529296/1‑49 (MQ=255)
                 tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:5377453/1‑49 (MQ=255)
                  cGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCa                           >  1:1728737/1‑50 (MQ=255)
                  cGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCa                           >  1:1886006/1‑50 (MQ=255)
                  cGGTCATATATTTCCACCAACGTTAGCAGACATCAGCGCAGACAACCGcc                           >  2:2557073/1‑49 (MQ=255)
                   ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          >  1:419786/1‑50 (MQ=255)
                    gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                         >  1:5222383/1‑50 (MQ=255)
                    gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                         >  2:871028/1‑50 (MQ=255)
                     tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  2:4547099/1‑50 (MQ=255)
                     tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  2:4746930/1‑50 (MQ=255)
                     tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  2:373032/1‑50 (MQ=255)
                     tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  1:1099686/1‑50 (MQ=255)
                      cATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  2:684001/1‑49 (MQ=255)
                      cATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  1:67266/1‑49 (MQ=255)
                       atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  1:3537780/1‑48 (MQ=255)
                       atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  2:220605/1‑48 (MQ=255)
                       atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  1:897011/1‑48 (MQ=255)
                        tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCTGCCGCATTCCAg                     <  1:3144396/50‑1 (MQ=255)
                        tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg                     >  1:1642988/1‑50 (MQ=255)
                        tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg                     >  2:3407194/1‑50 (MQ=255)
                         atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    >  1:5749164/1‑50 (MQ=255)
                         atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    >  1:2032557/1‑50 (MQ=255)
                         atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    >  1:1235180/1‑50 (MQ=255)
                          tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    <  1:4342201/49‑1 (MQ=255)
                          tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCg                   >  1:1071189/1‑50 (MQ=255)
                          tatTTCCACCAACGTTGGAAGACAGCAGTGCTGGCCACCGCATTCCAGCg                   <  1:1315584/50‑1 (MQ=255)
                           atTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGt                  >  1:5253464/1‑50 (MQ=255)
                           atTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGt                  >  1:933293/1‑50 (MQ=255)
                            tttCCACCAACGTTGGCAGACATCAGTGCTGGCATCCGCATTCCAGCGtt                 <  1:2917453/50‑1 (MQ=255)
                            tttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGt                  <  1:3156732/49‑1 (MQ=255)
                             ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc                >  2:2590985/1‑50 (MQ=255)
                              tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttct               >  2:4233118/1‑50 (MQ=255)
                               ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:819901/1‑50 (MQ=255)
                                caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:1020925/1‑49 (MQ=255)
                                caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  2:288332/1‑49 (MQ=255)
                                caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  2:3477461/1‑49 (MQ=255)
                                caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              <  2:5288974/49‑1 (MQ=255)
                                 accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  2:2829987/1‑48 (MQ=255)
                                 accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCa            <  2:3453050/50‑1 (MQ=255)
                                  ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:2282143/1‑47 (MQ=255)
                                  ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg           <  1:3789947/50‑1 (MQ=255)
                                  ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg           <  2:5532089/50‑1 (MQ=255)
                                  ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg           <  1:1171094/50‑1 (MQ=255)
                                   caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:5115475/1‑46 (MQ=255)
                                    aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTACAGCGTTCTTCAGAt         <  1:458222/50‑1 (MQ=255)
                                     aCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt        <  1:2934395/50‑1 (MQ=255)
                                     aCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt        >  2:357695/1‑50 (MQ=255)
                                      cGTTGGCAGACATCAGTGCTGGCGACCGCATTCCAGCGTTCTTCAGATTc       <  1:5298316/50‑1 (MQ=255)
                                       gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc       >  2:1381629/1‑49 (MQ=255)
                                       gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCa      <  2:429738/50‑1 (MQ=255)
                                        ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCaa     <  1:2867347/50‑1 (MQ=255)
                                        ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCaa     >  1:362822/1‑50 (MQ=255)
                                         tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt    >  2:4286603/1‑50 (MQ=255)
                                          ggCAGACATCAGTGCTGGCATCCGCATTCCAGCGTTCTTCAGATTCAAt    <  2:2056326/49‑1 (MQ=39)
                                          ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc   >  1:2791308/1‑50 (MQ=255)
                                          ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc   >  2:3627724/1‑50 (MQ=255)
                                          ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc   >  2:4272741/1‑50 (MQ=255)
                                           gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct  >  1:37774/1‑50 (MQ=255)
                                           gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct  <  2:4124591/50‑1 (MQ=255)
                                           gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct  >  1:4466680/1‑50 (MQ=255)
                                              |                                              
GGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093177‑4093269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑