BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	489.9 / NA	121	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (61/60); total (61/60)

Only 100 of 121 total aligned reads displayed.

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  2:4516723/1‑50 (MQ=255)

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  2:5280511/1‑50 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             <  1:4254641/49‑1 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  2:4843118/1‑49 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           <  2:5762282/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  2:730838/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  2:4324749/50‑1 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         <  2:3415363/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        >  1:3507541/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        >  2:5690121/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  2:3072119/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:4206214/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:5239257/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  2:2829376/50‑1 (MQ=255)

       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:5106485/1‑50 (MQ=255)

       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  2:3841377/1‑50 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  2:3081556/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:2553672/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:4442904/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:5218279/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  2:4762732/1‑50 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:1218933/1‑50 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  2:143682/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:4690090/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  2:5311077/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                   <  2:4271847/50‑1 (MQ=255)

           gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  2:5817706/1‑48 (MQ=255)

           gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  2:4985048/1‑48 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATGTTCTACAg                                 <  1:331681/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  2:4305507/49‑1 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 >  1:2304006/1‑49 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               <  2:2346581/50‑1 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                >  1:1133787/1‑49 (MQ=255)

              tttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                >  1:1954956/1‑49 (MQ=255)

               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:309137/1‑49 (MQ=255)

               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  2:2626703/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  1:2566145/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             <  1:642953/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           <  1:154145/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:5671350/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  2:1221964/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  2:3658634/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:5781183/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:653331/50‑1 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  2:5491702/1‑50 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:5519703/1‑50 (MQ=255)

                      aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       >  1:1530998/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       <  1:3382094/49‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       <  2:1759689/49‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       <  2:2426257/49‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      >  2:2147981/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      <  2:62265/50‑1 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      >  2:1678693/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  2:17217/50‑1 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  2:813762/1‑49 (MQ=255)

                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   <  1:1219397/50‑1 (MQ=255)

                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   <  1:2887026/50‑1 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:3528787/1‑49 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:3615404/1‑49 (MQ=255)

                            aaaCAGACTATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:152827/1‑50 (MQ=39)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 <  1:1232678/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  2:361078/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:2797287/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:5706537/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  2:3051987/1‑50 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  1:1679427/1‑50 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  1:4111070/1‑50 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               <  1:2193366/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               <  2:5833504/50‑1 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              >  1:5806938/1‑50 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              <  1:3063675/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:3772960/1‑50 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:5349582/1‑49 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  2:3734764/50‑1 (MQ=255)

                                  aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa           <  1:3797359/50‑1 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa           >  2:4250451/1‑49 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa           >  2:5252807/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  2:5293001/50‑1 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:2874713/1‑49 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:366992/1‑48 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       <  1:2125321/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       <  2:482551/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:806595/1‑50 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:714115/1‑50 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  2:1916423/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  2:4910376/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  2:4649846/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  2:4915823/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:2093873/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  2:5691602/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    <  1:5252238/50‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    <  1:5814795/50‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    <  2:725617/50‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   >  2:4755359/1‑49 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   >  1:3099342/1‑49 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  2:256385/49‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  2:3371538/50‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  1:4972842/50‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  1:1010925/50‑1 (MQ=255)

                                            gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  1:1458543/1‑49 (MQ=255)

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`