BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,485,906	C→A	A16S (GCC→TCC)	pepA ←	aminopeptidase A/I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,485,906	0	C	A	99.0%	761.5 / NA	196	A16S (GCC→TCC)	pepA	aminopeptidase A/I
Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (92/102); total (94/102)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 196 total aligned reads displayed.

AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG  >  NC_000913/4485859‑4485952

aaTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAAc                                              >  1:1715528/1‑50 (MQ=255)

 aTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACt                                             <  2:3649555/50‑1 (MQ=255)

    ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTcc                                           <  1:1992989/49‑1 (MQ=255)

    ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg                                          <  1:5097804/50‑1 (MQ=255)

    ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg                                          <  2:1593238/50‑1 (MQ=255)

     gagaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGc                                         <  2:191858/50‑1 (MQ=255)

     gagaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGc                                         >  1:5847181/1‑50 (MQ=255)

     gagaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGc                                         <  1:3920160/50‑1 (MQ=255)

     gagaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGc                                         <  2:663931/50‑1 (MQ=255)

      agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                        <  1:4927521/50‑1 (MQ=255)

      agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                        <  2:3765489/50‑1 (MQ=255)

      agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                        <  2:2660172/50‑1 (MQ=255)

      agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                        >  2:3988903/1‑50 (MQ=255)

      agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                        <  2:4819226/50‑1 (MQ=255)

      agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                        >  2:5505702/1‑50 (MQ=255)

      agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                        >  1:3461624/1‑50 (MQ=255)

       gaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTg                                       >  1:630354/1‑50 (MQ=255)

        aaaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGt                                      >  1:923362/1‑50 (MQ=255)

        aaaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGt                                      >  1:547997/1‑50 (MQ=255)

        aaaGGCGACGCGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGt                                      >  2:1957501/1‑50 (MQ=255)

         aaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGtt                                     <  1:266987/50‑1 (MQ=255)

         aaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGtt                                     <  1:3596078/50‑1 (MQ=255)

          aGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGttt                                    <  2:2087376/50‑1 (MQ=255)

           ggCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtc                                   <  1:3357230/50‑1 (MQ=255)

           ggCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtc                                   >  2:1415265/1‑50 (MQ=255)

           ggCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtc                                   <  1:5436331/50‑1 (MQ=255)

            gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct                                  <  2:5048717/50‑1 (MQ=255)

             cGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtctc                                 >  1:3079149/1‑50 (MQ=255)

             cGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtctc                                 <  1:3297987/50‑1 (MQ=255)

              gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                                <  1:920213/50‑1 (MQ=255)

              gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                                >  1:3857346/1‑50 (MQ=255)

              gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                                >  1:4763430/1‑50 (MQ=255)

              gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                                <  1:2035822/50‑1 (MQ=255)

               aCGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCg                               >  1:3720203/1‑50 (MQ=255)

               aCGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCg                               <  1:3109451/50‑1 (MQ=255)

               aCGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCg                               >  2:3748836/1‑50 (MQ=255)

                cGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCgg                              <  2:149916/50‑1 (MQ=255)

                agTGGTTCGAAGACGCTCACGACGATGCAGGAACGCCGCTGTTTCTCCgg                              >  2:231790/2‑50 (MQ=255)

                 gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg                             <  1:4785376/50‑1 (MQ=255)

                 gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg                             >  1:4484933/1‑50 (MQ=255)

                 gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg                             >  2:4913544/1‑50 (MQ=255)

                  tGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc                            >  2:5267606/1‑50 (MQ=255)

                      tCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                        >  2:1327208/1‑50 (MQ=255)

                       cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                        <  2:1175767/49‑1 (MQ=255)

                       cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                        <  2:1860551/49‑1 (MQ=255)

                       cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                        <  2:4959737/49‑1 (MQ=255)

                       cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                        <  2:5480105/49‑1 (MQ=255)

                       cGAACACGCCCACGGCGATACGGGAACTTCTCTGTTTCTCCGGGCTAcc                        <  2:345492/49‑1 (MQ=16)

                        gAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCg                       <  1:2482618/49‑1 (MQ=255)

                        gAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGc                      >  1:1056284/1‑50 (MQ=255)

                         aaGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt                     >  1:3794788/1‑50 (MQ=255)

                         aaGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt                     >  2:4597471/1‑50 (MQ=255)

                          aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                    <  2:2794490/50‑1 (MQ=255)

                          aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                    <  2:3632348/50‑1 (MQ=255)

                            aCGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt                  >  1:1051949/1‑50 (MQ=255)

                             cGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttttt                 <  2:2551636/50‑1 (MQ=255)

                              gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                >  2:4079554/1‑50 (MQ=255)

                              gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                <  2:4417940/50‑1 (MQ=255)

                              gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                <  2:851384/50‑1 (MQ=255)

                               cccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTac               <  1:5596549/50‑1 (MQ=255)

                               cccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                >  2:4185735/1‑49 (MQ=255)

                               cccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                >  1:423227/1‑49 (MQ=255)

                                ccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTaca              >  2:5752966/1‑50 (MQ=255)

                                ccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTaca              >  2:5103529/1‑50 (MQ=255)

                                ccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTaca              <  1:2787312/50‑1 (MQ=255)

                                 cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             <  2:2718098/50‑1 (MQ=255)

                                  acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             >  2:4652068/1‑49 (MQ=255)

                                  acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             >  2:4245249/1‑49 (MQ=255)

                                  acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             >  1:535129/1‑49 (MQ=255)

                                   cgacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACt            >  2:727928/1‑49 (MQ=255)

                                   cgacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACt            >  1:1462953/1‑49 (MQ=255)

                                   cgacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa           <  2:1830391/50‑1 (MQ=255)

                                   cgacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa           <  2:566452/50‑1 (MQ=255)

                                    gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaa          <  1:1022467/50‑1 (MQ=255)

                                    gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaa          <  2:1038675/50‑1 (MQ=255)

                                     acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaaa         >  1:90209/1‑50 (MQ=255)

                                      cgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAAc        >  2:5120017/1‑50 (MQ=255)

                                       gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt       <  1:5290474/50‑1 (MQ=255)

                                       gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt       <  2:3334932/50‑1 (MQ=255)

                                       gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt       <  2:1669719/50‑1 (MQ=255)

                                       gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt       >  2:2802198/1‑50 (MQ=255)

                                       gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt       >  1:2242027/1‑50 (MQ=255)

                                       gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt       <  2:2160901/50‑1 (MQ=255)

                                       gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt       <  1:2592652/50‑1 (MQ=255)

                                        aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc      <  1:960333/50‑1 (MQ=255)

                                         tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTcc     <  1:1845210/50‑1 (MQ=255)

                                         tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTcc     <  2:5789877/50‑1 (MQ=255)

                                         tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTcc     <  2:1793265/50‑1 (MQ=255)

                                          gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa    >  1:3167825/1‑50 (MQ=255)

                                          gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa    >  2:5337057/1‑50 (MQ=255)

                                           cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa    <  1:4986908/49‑1 (MQ=255)

                                           cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa    <  1:4367840/49‑1 (MQ=255)

                                           cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt   <  2:2630099/50‑1 (MQ=255)

                                           cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt   >  1:136/1‑50 (MQ=255)

                                           cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt   <  1:1161952/50‑1 (MQ=255)

                                            aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  <  1:5829333/50‑1 (MQ=255)

                                            aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  <  1:3283654/50‑1 (MQ=255)

                                            aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  <  1:5761763/50‑1 (MQ=255)

                                            aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  <  1:3335652/50‑1 (MQ=255)

                                             ggCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  >  1:2189304/1‑49 (MQ=255)

AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG  >  NC_000913/4485859‑4485952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`