Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	424.9 / NA	88	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (41/47);  total (41/47)

GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234                                                  |                                              gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:2171694/1‑50 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               <  1:2788237/50‑1 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:6688549/1‑50 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                              <  1:3301847/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:528489/1‑49 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:4261045/1‑49 (MQ=255)     tttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:307693/1‑47 (MQ=255)     tttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:4194616/1‑47 (MQ=255)      ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                            <  1:1717152/48‑3 (MQ=255)      ttttGTGCTTAGCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                            <  1:2205063/48‑3 (MQ=38)       tttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:327084/1‑50 (MQ=39)       tttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:5960417/1‑50 (MQ=39)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        <  1:5563277/49‑1 (MQ=38)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                                       <  1:3510041/49‑1 (MQ=38)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                                       <  1:1981900/49‑1 (MQ=38)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTc                                       <  1:5292339/49‑1 (MQ=38)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:3749876/1‑50 (MQ=39)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      <  1:1735149/49‑1 (MQ=38)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     >  1:4170675/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     <  1:1271651/49‑1 (MQ=38)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:5360812/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    <  1:6049664/50‑1 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    <  1:5888769/50‑1 (MQ=39)            gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   >  1:4759212/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:2188146/1‑50 (MQ=39)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:3044329/1‑49 (MQ=38)               tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                >  1:1694109/1‑50 (MQ=39)               tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                >  1:292247/1‑50 (MQ=39)               tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                >  1:3869149/1‑50 (MQ=39)                aTCGCGATGATTTTCGCTGTGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               >  1:2807855/1‑50 (MQ=255)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:4633409/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:2410067/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                <  1:755897/49‑1 (MQ=38)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               >  1:65225/1‑49 (MQ=38)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               <  1:105460/49‑1 (MQ=38)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               <  1:264600/49‑1 (MQ=38)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               <  1:1683027/49‑1 (MQ=38)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAt                            <  1:3726029‑M1/50‑2 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:1084132‑M1/1‑49 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                           <  1:103026‑M1/50‑3 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                           <  1:5234934‑M1/50‑3 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:2938556‑M1/1‑48 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:1277692‑M1/1‑48 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:4781732‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:1902844‑M1/50‑4 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:1007371‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:1133850‑M1/1‑46 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:1980238‑M1/50‑6 (MQ=255)                        gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       >  1:347398‑M1/1‑44 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      <  1:839660‑M1/50‑8 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:1046880‑M1/1‑43 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      <  1:5263898‑M1/50‑8 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      <  1:1546828‑M1/50‑8 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:3975267‑M1/1‑43 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:2398455‑M1/1‑43 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       <  1:2861345‑M1/49‑7 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:4165248‑M1/1‑42 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    >  1:1667285‑M1/1‑40 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    <  1:6438579‑M1/49‑10 (MQ=255)                             tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaa                   <  1:4944180‑M1/49‑11 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:6429516‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:1492047‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:2562749‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:250712‑M1/49‑12 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:847085‑M1/49‑12 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:4599003‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:1525986‑M1/1‑37 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:7014129‑M1/1‑37 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:1735110‑M1/1‑37 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:1814400‑M1/1‑37 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:3788008‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:5216262‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:1903730‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 <  1:88281‑M1/49‑13 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 <  1:4754801‑M1/49‑13 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               <  1:4183877‑M1/50‑15 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:2080328‑M1/1‑36 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:2086712‑M1/1‑36 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            >  1:5923812‑M1/1‑33 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            <  1:4236235‑M1/50‑18 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           <  1:4902380‑M1/50‑19 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:1812829‑M1/50‑20 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:1372901‑M1/50‑20 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:272690‑M1/1‑30 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGCATTAtagcaatcaaaccgaagcca         >  1:3972001‑M1/1‑30 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       <  1:2502090‑M1/50‑23 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       <  1:3659866‑M1/50‑23 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccac        <  1:637951‑M1/49‑22 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         <  1:4828231‑M1/48‑21 (MQ=255)                                         cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:2667188‑M1/1‑27 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     >  1:1965533‑M1/1‑26 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     >  1:2725854‑M1/1‑26 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccagatat    <  1:6838062‑M1/50‑26 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   <  1:5885697‑M1/50‑27 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   <  1:6445493‑M1/50‑27 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  >  1:1384168‑M1/1‑23 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  <  1:6544119‑M1/50‑28 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  >  1:6770720‑M1/1‑23 (MQ=255)                                                  |                                              GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑