Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	356.8 / NA	87	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/45);  total (42/45)

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857                                               |                                               atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                                             <  1:3256330/50‑1 (MQ=255)  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:5324972/50‑1 (MQ=255)  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:5804215/50‑1 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:3252815/1‑50 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:6790545/1‑50 (MQ=255)    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:998713/1‑49 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:2221749/50‑1 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:4052472/50‑1 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  1:3255177/1‑50 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  1:6411632/1‑50 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:2955587/50‑1 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:3463818/50‑1 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  1:3046536/1‑50 (MQ=255)      aTAGGCGGCAAACAGTGCCAGATGCGCCGCGCCATTGAGCATGTTAGTAc                                        >  1:1689402/1‑50 (MQ=39)       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  1:7003878/50‑1 (MQ=255)       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       >  1:6361999/1‑50 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      <  1:3138824/49‑1 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     >  1:4676091/1‑50 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:4544509/50‑1 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     >  1:4079393/1‑50 (MQ=255)         ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     >  1:3680257/1‑50 (MQ=255)          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:652378/50‑1 (MQ=255)          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:731902/50‑1 (MQ=255)           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  1:563967/1‑50 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  <  1:4704060/50‑1 (MQ=255)            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  <  1:6358840/50‑1 (MQ=255)             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 <  1:2804785/50‑1 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:5199642/1‑49 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:2503351/1‑49 (MQ=255)               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:4236146/1‑49 (MQ=255)                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                              <  1:7049162/50‑1 (MQ=255)                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:4641517/50‑1 (MQ=255)                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:4092399/50‑1 (MQ=255)                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                            <  1:4924260/50‑1 (MQ=255)                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                            <  1:3998622/50‑1 (MQ=255)                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:5780909/1‑49 (MQ=255)                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:4695154/1‑49 (MQ=255)                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:996517/1‑49 (MQ=255)                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:6597535/1‑48 (MQ=255)                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                           <  1:4929995/50‑1 (MQ=255)                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:6894515/1‑50 (MQ=255)                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:114050/50‑1 (MQ=255)                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:5193133/1‑49 (MQ=255)                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:785204/1‑48 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:5196850/1‑47 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                       <  1:4567663/50‑1 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:1640592/49‑1 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:6855299/49‑1 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:6825829/49‑1 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:6197846/1‑46 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:6054979/50‑1 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:952689/50‑1 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:3218463/50‑1 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                    >  1:5314876/1‑50 (MQ=255)                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  1:1192465/50‑1 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:6831527/1‑49 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:6737261/1‑49 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc                <  1:5650443/50‑1 (MQ=255)                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:2084283/1‑50 (MQ=255)                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               <  1:5238295/50‑1 (MQ=255)                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:4224109/1‑49 (MQ=255)                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:76068/1‑48 (MQ=255)                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:5959639/1‑48 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:1660292/48‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:6709243/49‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:5610427/49‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:2843704/49‑1 (MQ=255)                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  1:2887483/1‑50 (MQ=255)                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  1:2822424/50‑1 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:6227761/1‑50 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         <  1:811937/50‑1 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:6366864/1‑50 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:6181403/1‑50 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:1916628/50‑1 (MQ=255)                                        ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa      <  1:2157816/50‑1 (MQ=255)                                        ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:1438595/1‑49 (MQ=255)                                         tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:409588/1‑48 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     <  1:302694/49‑1 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     <  1:130575/49‑1 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:1949758/1‑47 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:1839460/1‑47 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:6282202/1‑47 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  1:6967645/1‑48 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  1:6438090/1‑48 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:4274560/49‑1 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:337210/49‑1 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  1:1618676/50‑1 (MQ=255)                                               |                                               ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑