Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	329.1 / NA	81	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (38/43);  total (38/43)

CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742711‑1742800                                             |                                              cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          <  1:4806374/50‑1 (MQ=255) cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           <  1:2817949/49‑1 (MQ=255) cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:2314096/1‑49 (MQ=255) cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:1864851/1‑49 (MQ=255) cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           <  1:648844/49‑1 (MQ=255) cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           <  1:4217449/49‑1 (MQ=255) cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           <  1:1441591/49‑1 (MQ=255)  gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:266383/1‑49 (MQ=255)  gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:1823490/1‑49 (MQ=255)   ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                        <  1:5492115/50‑1 (MQ=255)   ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                         >  1:5367507/1‑49 (MQ=255)    cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                       <  1:3859550/50‑1 (MQ=255)     aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:143962/50‑1 (MQ=255)     aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:6002661/50‑1 (MQ=255)        ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   >  1:5963749/1‑50 (MQ=255)        ttttttCTTCCCAAGAGACGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   >  1:4361699/1‑50 (MQ=255)         tttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  >  1:3844750/1‑50 (MQ=255)          ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:6304546/49‑1 (MQ=255)          ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:5399325/49‑1 (MQ=255)            ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                <  1:4524195/49‑1 (MQ=255)              cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                              <  1:1953503/49‑1 (MQ=255)               ttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             >  1:5968265/1‑49 (MQ=255)                tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           >  1:4755245/1‑50 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           <  1:2904598/49‑1 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             <  1:6782665/47‑1 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          >  1:3470022/1‑50 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          >  1:1355648/1‑50 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          >  1:507722/1‑50 (MQ=255)                  ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         <  1:2999839/50‑1 (MQ=255)                  ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         >  1:4695086/1‑50 (MQ=255)                  ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          <  1:4843226/49‑1 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:2390699/50‑1 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:7101697/50‑1 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:4815644/50‑1 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        >  1:4034635/1‑50 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        >  1:4030363/1‑50 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:3712495/50‑1 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:2356584/50‑1 (MQ=255)                    aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       >  1:2251075/1‑50 (MQ=255)                     agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:1744400/1‑50 (MQ=255)                     agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      <  1:4911103/50‑1 (MQ=255)                     agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:2326741/1‑50 (MQ=255)                     agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      <  1:1560267/50‑1 (MQ=255)                       agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:1720907/50‑1 (MQ=255)                        gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   >  1:135141/1‑50 (MQ=255)                        gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   <  1:248536/50‑1 (MQ=255)                         tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:1791879/50‑1 (MQ=255)                          cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:855751/1‑50 (MQ=255)                          cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:4100558/1‑50 (MQ=255)                          cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:2976126/1‑50 (MQ=255)                          cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:2826355/1‑50 (MQ=255)                           gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 <  1:3270953/49‑1 (MQ=255)                           gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:1366458/1‑50 (MQ=255)                           gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:609101/1‑50 (MQ=255)                            tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg               <  1:711279/50‑1 (MQ=255)                              cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             <  1:124258/50‑1 (MQ=255)                              cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             >  1:4875324/1‑50 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:4617167/1‑50 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:455066/1‑50 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            <  1:4486683/50‑1 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            <  1:2506373/50‑1 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:5540102/1‑50 (MQ=255)                                ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           <  1:1380138/50‑1 (MQ=255)                                ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           <  1:3351225/50‑1 (MQ=255)                                 tCCAGTGTTTCCCGAATTAAATGGACGCACAAACGCGTTGGCGTGACTTc          >  1:1054255/1‑50 (MQ=255)                                 tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          >  1:6737373/1‑50 (MQ=255)                                  ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:5890402/49‑1 (MQ=255)                                  ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:4119530/49‑1 (MQ=255)                                  ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         >  1:5695716/1‑50 (MQ=255)                                  ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         >  1:5120069/1‑50 (MQ=255)                                  ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         >  1:445107/1‑50 (MQ=255)                                   cAGTGTTGCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         <  1:4530723/49‑1 (MQ=255)                                    aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:1036368/1‑50 (MQ=255)                                    aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:4476331/1‑50 (MQ=255)                                     gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg      <  1:3586611/50‑1 (MQ=255)                                        tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    <  1:5544659/49‑1 (MQ=255)                                        tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    <  1:202002/49‑1 (MQ=255)                                        tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    <  1:6681132/49‑1 (MQ=255)                                        tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg   >  1:13694/1‑50 (MQ=255)                                        tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg   <  1:6870438/50‑1 (MQ=255)                                          tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  <  1:6151463/49‑1 (MQ=255)                                             |                                              CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742711‑1742800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑