Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 257,794 T→C intergenic (+23/‑35) frsA → / → crl fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913257,7940TC100.0% 486.4 / NA 115intergenic (+23/‑35)frsA/crlfermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (66/49);  total (66/49)

Only 100 of 115 total aligned reads displayed.

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACC  >  NC_000913/257747‑257839
                                               |                                             
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                             >  1:6777061/1‑50 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                             <  1:3566479/50‑1 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            >  1:2602147/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            >  1:713894/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            >  1:1311939/1‑50 (MQ=255)
  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            >  1:6362975/1‑49 (MQ=255)
  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            >  1:3535415/1‑49 (MQ=255)
  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                           <  1:6882488/50‑1 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            <  1:1125781/48‑1 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            <  1:5904108/48‑1 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                           <  1:7061639/49‑1 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                           >  1:5309351/1‑49 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                           >  1:5501189/1‑49 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                          <  1:1091773/50‑1 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                          >  1:114924/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                         >  1:6588450/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                         >  1:4257999/1‑49 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:3152897/1‑50 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:5934396/1‑50 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:6463736/1‑49 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                      <  1:5720642/50‑1 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:6813962/1‑48 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:2812340/1‑48 (MQ=255)
         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                    <  1:5321349/50‑1 (MQ=255)
         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                    <  1:3043389/50‑1 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                   <  1:3745963/50‑1 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                   >  1:3592038/1‑50 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                   <  1:4915549/50‑1 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                   >  1:6581787/1‑50 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                 >  1:6172568/1‑50 (MQ=255)
             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGc                                <  1:2180986/50‑1 (MQ=255)
              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                               >  1:2259989/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                              >  1:6408390/1‑50 (MQ=255)
                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                              >  1:1394556/1‑49 (MQ=255)
                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGAAAAACAGTTGCAt                              >  1:2492916/1‑49 (MQ=38)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                            <  1:2038650/49‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                            >  1:4821731/1‑49 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                             <  1:461064/48‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                             <  1:100493/48‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                             <  1:858575/48‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                          <  1:5137935/50‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                          <  1:5044052/50‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                          <  1:4434036/50‑1 (MQ=255)
                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                           >  1:5431961/1‑49 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                        <  1:4432870/50‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                        <  1:2503548/50‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                         <  1:4191549/49‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                         <  1:5766871/49‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                         <  1:3483598/49‑1 (MQ=255)
                      tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                         >  1:2932517/1‑48 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:1352937/1‑50 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaccg                      >  1:3823858/1‑47 (MQ=39)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAgg                     <  1:5309886/50‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAgg                     <  1:1327666/50‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:113029/1‑49 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      <  1:1286225/49‑1 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGga                    <  1:2069032/50‑1 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                   <  1:4875131/50‑1 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:4615637/1‑46 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:3066507/1‑46 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:4337106/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:810979/1‑49 (MQ=255)
                             ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:6407200/1‑48 (MQ=255)
                             ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:4044797/1‑48 (MQ=255)
                             ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:567647/1‑48 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                <  1:73881/49‑1 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                <  1:180487/49‑1 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                <  1:2273515/49‑1 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                <  1:2323528/49‑1 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg               >  1:5246084/1‑50 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg               >  1:6733213/1‑50 (MQ=255)
                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg               >  1:579971/1‑50 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCa             <  1:6656760/50‑1 (MQ=255)
                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa            >  1:6157185/1‑50 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa            <  1:1077993/48‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa            <  1:855207/48‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt           <  1:3420760/49‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg          <  1:375366/50‑1 (MQ=255)
                                   aaTTGTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg          <  1:332430/50‑1 (MQ=39)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa         <  1:2891367/50‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa         <  1:446470/50‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa         >  1:2989179/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa         >  1:4422460/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa         >  1:6497695/1‑50 (MQ=255)
                                      tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg       >  1:4725151/1‑50 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      >  1:2173909/1‑49 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      <  1:1290440/49‑1 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      <  1:6451475/49‑1 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      >  1:3301520/1‑49 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt      <  1:5650083/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt     <  1:6460583/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt     <  1:5450920/49‑1 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa    <  1:5710334/49‑1 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc   >  1:4887347/1‑50 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc   >  1:116886/1‑50 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc   >  1:3955645/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc  >  1:5559294/1‑50 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc  >  1:3570923/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc  >  1:2182642/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc  >  1:5637121/1‑49 (MQ=255)
                                               |                                             
CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACC  >  NC_000913/257747‑257839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑