BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	440.3 / NA	108	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (51/57); total (51/57)

Only 100 of 108 total aligned reads displayed.

TCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512956‑3513048

tcgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                             >  1:3876530/1‑50 (MQ=255)

tcgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                             <  1:2884044/50‑1 (MQ=255)

 cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:12457/1‑50 (MQ=255)

  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  1:3926589/1‑50 (MQ=255)

  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           <  1:3050534/50‑1 (MQ=255)

  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           <  1:5618020/50‑1 (MQ=255)

  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            <  1:1714534/49‑1 (MQ=255)

    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:4084368/50‑1 (MQ=255)

    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:1832713/50‑1 (MQ=255)

    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:5171679/1‑49 (MQ=255)

     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:4234820/1‑48 (MQ=255)

     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:2018591/1‑48 (MQ=255)

     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:1831143/50‑1 (MQ=255)

        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:525846/50‑1 (MQ=255)

        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:2524969/50‑1 (MQ=255)

        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:6743136/50‑1 (MQ=255)

        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:5141882/49‑1 (MQ=255)

        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:2858084/49‑1 (MQ=255)

         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:4535665/1‑50 (MQ=255)

         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:6327297/1‑50 (MQ=255)

          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:1048831/50‑1 (MQ=255)

          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:5525364/1‑50 (MQ=255)

          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:1507617/50‑1 (MQ=255)

            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:5018009/50‑1 (MQ=255)

            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:5335726/50‑1 (MQ=255)

            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:5917933/50‑1 (MQ=255)

             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCg                                <  1:3147942/50‑1 (MQ=255)

               gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              <  1:7106467/50‑1 (MQ=255)

                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:1475987/1‑49 (MQ=255)

                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:3665199/1‑49 (MQ=255)

                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:6486448/1‑49 (MQ=255)

                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:4422097/1‑49 (MQ=255)

                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:2405834/50‑1 (MQ=255)

                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:147575/50‑1 (MQ=255)

                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  1:1626825/1‑50 (MQ=255)

                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:1333583/50‑1 (MQ=255)

                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:2276279/50‑1 (MQ=255)

                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:3831377/50‑1 (MQ=255)

                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:3994633/1‑50 (MQ=255)

                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:909873/1‑50 (MQ=255)

                     ttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:4596235/1‑49 (MQ=255)

                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       <  1:5056784/49‑1 (MQ=255)

                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       <  1:917950/49‑1 (MQ=255)

                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:5272319/1‑50 (MQ=255)

                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:562942/1‑50 (MQ=255)

                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:6746538/1‑50 (MQ=255)

                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:6037075/1‑50 (MQ=255)

                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     <  1:2590466/50‑1 (MQ=255)

                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:5038277/1‑50 (MQ=255)

                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     <  1:1959311/50‑1 (MQ=255)

                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      <  1:4417145/49‑1 (MQ=255)

                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAGCGTTATTg                    <  1:1756285/50‑1 (MQ=255)

                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    >  1:1954595/1‑50 (MQ=255)

                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    >  1:6885259/1‑50 (MQ=255)

                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    >  1:5861671/1‑50 (MQ=255)

                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    <  1:1264498/50‑1 (MQ=255)

                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    <  1:2363493/50‑1 (MQ=255)

                         ttttCTTCACCACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    >  1:3042829/1‑50 (MQ=255)

                          tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:2192446/1‑50 (MQ=255)

                           ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   <  1:627391/49‑1 (MQ=255)

                           ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:6451442/1‑49 (MQ=255)

                           ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:4392430/1‑49 (MQ=255)

                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 <  1:4487596/49‑1 (MQ=255)

                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:5103942/1‑50 (MQ=255)

                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:3722345/1‑50 (MQ=255)

                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:3760738/1‑50 (MQ=255)

                              ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:4513740/1‑49 (MQ=255)

                               tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:2203646/1‑50 (MQ=255)

                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                <  1:4879854/47‑1 (MQ=255)

                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              <  1:460286/49‑1 (MQ=255)

                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:16424/1‑50 (MQ=255)

                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:148290/50‑1 (MQ=255)

                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:6475692/50‑1 (MQ=255)

                                  cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           <  1:3737893/50‑1 (MQ=255)

                                  cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           >  1:4633441/1‑50 (MQ=255)

                                   tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:128891/1‑50 (MQ=255)

                                    aCCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:5738795/1‑49 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:5605732/48‑1 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:2780628/48‑1 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:4055586/48‑1 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:4584678/49‑1 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCa        <  1:3595000/50‑1 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCa        <  1:1948680/50‑1 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCa        <  1:1410789/50‑1 (MQ=255)

                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCa        <  1:6625463/50‑1 (MQ=255)

                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:5935614/1‑47 (MQ=255)

                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAg       <  1:6069785/50‑1 (MQ=255)

                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAgc      >  1:6210930/1‑49 (MQ=255)

                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:221618/1‑50 (MQ=255)

                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:5622761/50‑1 (MQ=255)

                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:6686252/1‑50 (MQ=255)

                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:2874572/1‑50 (MQ=255)

                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:4434502/1‑50 (MQ=255)

                                         tttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt    >  1:1061687/1‑50 (MQ=255)

                                          ttCGGGTCTTCTAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtc   >  1:1500433/1‑49 (MQ=38)

                                           tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   <  1:1405914/49‑1 (MQ=255)

                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:2714707/49‑1 (MQ=255)

                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:4611227/49‑1 (MQ=255)

                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:79275/49‑1 (MQ=255)

                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:5251239/1‑49 (MQ=255)

TCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512956‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`