| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,715,660 | (G)5→6 | coding (238/588 nt) | eamB → | cysteine/O‑acetylserine exporter EamB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,715,655 | 1 | . | G | 75.2% | 314.4 / 111.5 | 109 | coding (233/588 nt) | eamB | cysteine/O‑acetylserine exporter EamB |
| Reads supporting (aligned to +/- strand): ref base . (12/15); new base G (28/54); total (40/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
TTTTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCC > NC_000913/2715606‑2715703 | ttttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc < 1:2906844/50‑1 (MQ=255)ttttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc < 2:182307/50‑1 (MQ=255)ttttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc > 2:1959748/1‑50 (MQ=255) tttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑g > 1:71773/1‑50 (MQ=255) ttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑gg > 2:2839663/1‑50 (MQ=255) ttCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑g < 2:4173955/49‑1 (MQ=255) tCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg > 1:4252413/1‑50 (MQ=255) cACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑g > 2:110545/1‑50 (MQ=255) cACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑g > 2:4478087/1‑50 (MQ=255) cACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑g > 1:50873/1‑50 (MQ=255) cACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑g > 1:2421950/1‑50 (MQ=255) cACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg < 2:181062/49‑1 (MQ=255) aCTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑gg > 1:2898846/1‑50 (MQ=255) aCTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑g < 1:1716650/49‑1 (MQ=255) cTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgt‑gc‑ggg‑ggg < 2:1352734/50‑2 (MQ=255) cTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑ggg > 1:1871516/1‑49 (MQ=255) tGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑gggc > 1:138329/1‑48 (MQ=255) tGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑ggcg > 1:44107/1‑50 (MQ=255) tGGCAGTGATTGACCCGGCAGCGGTACACCTTTTCAGTTgg‑gc‑ggg‑gggc > 1:4596542/1‑48 (MQ=39) gCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑gggc < 2:2619754/48‑3 (MQ=255) gCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑gggc < 1:3209113/48‑3 (MQ=255) gCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTgg‑gc‑ggg‑gggc < 1:349396/48‑3 (MQ=255) cAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCa > 1:213087/1‑50 (MQ=255) aGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCa > 1:2030716/1‑50 (MQ=255) aGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCa < 1:1206986/50‑1 (MQ=255) aGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCa > 2:3998089/1‑50 (MQ=255) gTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCat < 2:5553801/50‑1 (MQ=255) tGATTGACCCGGCGGCGGTACACCTTTGGAGTTGG‑GCGGGG‑GGCGGCata < 2:5432216/50‑1 (MQ=255) aTTGACCCGGCAGCGGTACGCCTTTTGAGTTGG‑GCGGGG‑GGCGGCatata < 2:4852694/50‑1 (MQ=255) aTTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCatata > 1:1290243/1‑50 (MQ=255) aTTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCatata < 2:5303555/50‑1 (MQ=255) aTTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCatat < 1:2476499/49‑1 (MQ=255) aTTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCatat < 2:1567928/49‑1 (MQ=255) aTTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCatatat < 1:942455/50‑1 (MQ=255) aTTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCatata < 2:4178000/49‑1 (MQ=255) aTTGACCCGGCAGCGGTACACCTTTTGAGGTGG‑GCGGGG‑GGCGGCatata > 2:1624247/1‑50 (MQ=255) ttGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCatatat < 1:5628771/50‑1 (MQ=255) ttGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATAtt < 1:1567228/50‑1 (MQ=255) tGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATAtt > 1:771277/1‑50 (MQ=255) gACCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTg > 2:4366303/1‑50 (MQ=255) gACCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTg < 2:4458215/49‑1 (MQ=255) tcccGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGt < 1:3170415/49‑1 (MQ=255) aCCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGt < 1:3047687/50‑1 (MQ=255) aCCCGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGt < 1:4485274/50‑1 (MQ=255) aCCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGt < 2:355064/49‑1 (MQ=255) aCCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTc > 2:779177/1‑50 (MQ=255) cccGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTc < 2:656427/50‑1 (MQ=255) ccGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTct > 2:476147/1‑50 (MQ=255) ccGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTct < 2:2601210/50‑1 (MQ=255) ccGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTctg < 2:4809160/50‑1 (MQ=255) cGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctg < 2:1086228/50‑1 (MQ=255) cGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctg > 1:3571613/1‑50 (MQ=255) cGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctg < 1:2328970/50‑1 (MQ=255) cGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctg < 2:3148084/50‑1 (MQ=255) cGGCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctg < 1:3667817/50‑1 (MQ=255) cGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTctgg < 2:3117604/50‑1 (MQ=255) ggCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctgg < 1:5132429/50‑1 (MQ=255) ggCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctgg > 2:3115839/1‑50 (MQ=255) gCAGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctggc > 1:2403103/1‑50 (MQ=255) cAGCGGTACGCCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctggct < 2:933030/50‑1 (MQ=255) cAGCGGTACACCTGTTGAGTTGG‑GCGGGG‑GGCGGCATGTATTGTgtggct < 2:900558/50‑1 (MQ=255) aGCGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTctggctg < 1:5305718/50‑1 (MQ=255) aGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTctggctgg > 1:5485427/1‑50 (MQ=255) aGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTctggctgg > 2:421344/1‑50 (MQ=255) cGGTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCtggctggc > 2:5069667/1‑50 (MQ=255) ggTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCg > 2:3692365/1‑50 (MQ=255) ggTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGt < 1:2596115/50‑1 (MQ=255) gTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGt < 1:1749685/50‑1 (MQ=255) gTACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGt > 1:207499/1‑50 (MQ=255) gTACACCTTTTGAGTTGG‑GC‑GGGTGGCGGCATATATTGTCTGGCTGGCGt < 1:3267778/50‑1 (MQ=255) gTACACCTTTTGAGTGGGCGG‑GGG‑GGCGGCGGATATTGCCTGGCTGCCGt > 2:377786/1‑50 (MQ=255) tACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTg > 1:3639807/1‑50 (MQ=255) tACACCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTg < 1:5113190/50‑1 (MQ=255) acacCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTg > 2:317731/1‑49 (MQ=39) cacCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTgg > 1:4642126/1‑49 (MQ=39) cacCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGa < 1:3431097/49‑1 (MQ=255) acCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGaa < 2:3535441/50‑1 (MQ=255) acCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGaa < 2:5425517/50‑1 (MQ=255) acCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGaa < 2:4873331/50‑1 (MQ=255) acCTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGaa < 1:180358/50‑1 (MQ=255) ccTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGaaa < 1:3921130/50‑1 (MQ=255) cTTTTGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGaaaa < 1:2263556/50‑1 (MQ=255) cTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAAt < 1:5598826/50‑1 (MQ=255) cTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAAt > 1:5345236/1‑50 (MQ=255) tttGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATc > 1:3420566/1‑50 (MQ=255) tttGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCg > 1:4590590/1‑50 (MQ=255) ttGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAAt < 1:439950/48‑1 (MQ=39) ttGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAAt < 1:4860594/48‑1 (MQ=39) ttGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATc < 1:2065012/48‑1 (MQ=255) tGAGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCg < 1:2733227/49‑1 (MQ=39) aGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGcca > 1:2743695/1‑50 (MQ=255) aGTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGcc < 1:3740100/49‑1 (MQ=39) gTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGccac > 2:4239116/1‑50 (MQ=255) gTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGccac < 2:1638258/50‑1 (MQ=255) gTTGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGccac < 1:1756317/50‑1 (MQ=255) gTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGccacc < 1:1465262/50‑1 (MQ=255) tGG‑GCGGGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGccac > 2:3719844/1‑48 (MQ=39) gg‑gc‑ggg‑ggcggCATATATTGTCTGGCTAGCGTGGAAAATCGCCACCAg < 2:3458425/49‑1 (MQ=255) g‑gc‑ggg‑ggcggCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGcc < 2:3776551/50‑1 (MQ=255) gc‑ggg‑ggcggCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGccc < 2:4938063/50‑1 (MQ=255) | TTTTCACTGGCAGTGATTGACCCGGCAGCGGTACACCTTTTGAGTTGG‑GC‑GGG‑GGCGGCATATATTGTCTGGCTGGCGTGGAAAATCGCCACCAGCCC > NC_000913/2715606‑2715703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |