Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,270,811 C→T V347M (GTG→ATG)  chaA ← Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,8110CT100.0% 453.7 / NA 114V347M (GTG→ATG) chaANa(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (64/50);  total (64/50)

Only 100 of 114 total aligned reads displayed.

CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857
                                               |                                              
catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  2:3308273/1‑50 (MQ=255)
catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  2:1474255/1‑50 (MQ=255)
  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  2:4357359/50‑1 (MQ=255)
    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  2:4811237/1‑49 (MQ=255)
    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                          <  2:314484/50‑1 (MQ=255)
     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         >  1:5100682/1‑50 (MQ=255)
     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:1559385/50‑1 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  2:4875138/1‑50 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  1:770238/1‑50 (MQ=255)
      aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  2:2200322/1‑50 (MQ=255)
       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       >  1:3577626/1‑50 (MQ=255)
       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  2:5040534/50‑1 (MQ=255)
        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:328102/1‑50 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    >  1:2454704/1‑50 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:2489343/50‑1 (MQ=255)
          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    >  2:1865043/1‑50 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  2:2304369/1‑50 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  2:5292820/50‑1 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  2:1393376/1‑50 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  2:4020400/1‑50 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  1:5209828/1‑50 (MQ=255)
           cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  1:2536080/1‑50 (MQ=255)
            ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  <  2:2598413/50‑1 (MQ=255)
             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   >  2:4717631/1‑48 (MQ=255)
             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 <  2:2984452/50‑1 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  1:4912246/50‑1 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  1:251719/50‑1 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  2:2077615/50‑1 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:1133982/1‑49 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:142700/1‑49 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:3914722/1‑49 (MQ=255)
               aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:3331197/1‑49 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:743055/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:1453678/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:1107780/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:2466919/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:2964423/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  2:5128823/1‑48 (MQ=255)
                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                              <  1:195431/50‑1 (MQ=255)
                aaCAGTGCCAGATGAGCTGCGCCATTGAGCATGTTAGTACGGCCGGTGga                              <  2:1467292/50‑1 (MQ=255)
                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  2:2745979/50‑1 (MQ=255)
                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:3539544/50‑1 (MQ=255)
                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:803546/50‑1 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                            <  1:4742738/50‑1 (MQ=255)
                  cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:3507398/1‑49 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:1767831/1‑48 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                           <  1:1561986/50‑1 (MQ=255)
                   aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                           <  1:1919477/50‑1 (MQ=255)
                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          <  2:2943793/50‑1 (MQ=255)
                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:3939517/1‑50 (MQ=255)
                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:211402/1‑50 (MQ=255)
                    gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:2884016/1‑50 (MQ=255)
                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  2:4145789/50‑1 (MQ=255)
                     tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:1935029/50‑1 (MQ=255)
                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  2:4958402/1‑48 (MQ=255)
                      gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:1050083/1‑48 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:4993083/1‑47 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:274752/1‑47 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                       <  1:2781992/50‑1 (MQ=255)
                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  2:4441326/49‑1 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:2871144/1‑46 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:3430384/50‑1 (MQ=255)
                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  2:2202991/50‑1 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  2:2846265/1‑49 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  1:3992147/1‑49 (MQ=255)
                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:5478057/50‑1 (MQ=255)
                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                    <  2:3597066/50‑1 (MQ=255)
                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  2:5639963/1‑50 (MQ=255)
                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  2:5644886/1‑50 (MQ=255)
                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  2:2012948/1‑50 (MQ=255)
                            tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  2:3994679/50‑1 (MQ=255)
                             gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:1668167/1‑50 (MQ=255)
                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  2:3061085/1‑49 (MQ=255)
                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc                <  1:5378730/50‑1 (MQ=255)
                               gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  2:2257891/1‑50 (MQ=255)
                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac              <  1:1101891/50‑1 (MQ=255)
                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac              <  1:3345323/50‑1 (MQ=255)
                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:3451578/1‑49 (MQ=255)
                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:5155864/1‑48 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  2:2769053/48‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  2:4633694/48‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:395158/48‑1 (MQ=255)
                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  2:4946536/50‑1 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  1:2794591/1‑50 (MQ=255)
                                    gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  1:3508512/50‑1 (MQ=255)
                                    gcCATTGAGCATGTTAGTAAGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  2:1601142/50‑1 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  2:2797170/1‑50 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:2354551/1‑50 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:5049075/1‑50 (MQ=255)
                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  2:2846158/1‑50 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:983338/1‑49 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  2:134407/50‑1 (MQ=255)
                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:5632393/50‑1 (MQ=255)
                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        >  2:4720407/1‑49 (MQ=255)
                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  2:3570276/50‑1 (MQ=255)
                                         tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:1324482/1‑48 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  1:2630860/1‑48 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  2:4302998/50‑1 (MQ=255)
                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:830954/49‑1 (MQ=255)
                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  2:4114508/50‑1 (MQ=255)
                                               |                                              
CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑