Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 119,028 T→C V99A (GTC→GCC)  ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913119,0280TC100.0% 465.6 / NA 122V99A (GTC→GCC) ampD1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (65/57);  total (65/57)

Only 100 of 136 total aligned reads displayed.

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074
                                               |                                              
 aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             >  2:3378330/1‑50 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:2109295/1‑50 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:802984/1‑50 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:4189781/1‑50 (MQ=255)
  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:5157929/50‑1 (MQ=255)
   aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:1731901/1‑49 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  1:2650300/49‑1 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  2:4100746/49‑1 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  2:808766/1‑48 (MQ=255)
    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCa                                          <  2:520550/50‑1 (MQ=255)
     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         <  1:341379/50‑1 (MQ=255)
     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  1:477287/1‑50 (MQ=255)
      ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  2:4544595/1‑49 (MQ=255)
       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  1:4356027/1‑50 (MQ=255)
        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:2117376/1‑50 (MQ=255)
        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:1800737/1‑50 (MQ=255)
        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  2:1942947/1‑50 (MQ=255)
        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  2:2710057/1‑50 (MQ=255)
         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                                     <  1:68236/50‑1 (MQ=255)
         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                                     <  1:1135872/50‑1 (MQ=255)
          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  2:1437767/1‑48 (MQ=255)
          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:2197500/1‑48 (MQ=255)
           aTGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   >  2:2577288/1‑50 (MQ=255)
            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  <  1:5306841/50‑1 (MQ=255)
            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  >  2:4752418/1‑50 (MQ=255)
            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  >  2:1526975/1‑50 (MQ=255)
            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   <  2:3876441/49‑1 (MQ=255)
            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   <  2:3183477/49‑1 (MQ=255)
             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  2:5368688/1‑50 (MQ=255)
             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:1105308/1‑50 (MQ=255)
             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  2:5411259/1‑50 (MQ=255)
             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:212770/1‑50 (MQ=255)
              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                >  2:5629853/1‑50 (MQ=255)
              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 <  2:1887115/49‑1 (MQ=255)
               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  1:2391343/1‑50 (MQ=255)
                ccTTTTGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              >  2:477577/1‑50 (MQ=255)
                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              >  2:4919642/1‑50 (MQ=255)
                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              >  1:4369497/1‑50 (MQ=255)
                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               <  1:4101952/49‑1 (MQ=255)
                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  1:1921057/50‑1 (MQ=255)
                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                              <  1:4618451/49‑1 (MQ=255)
                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:669401/1‑49 (MQ=255)
                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  2:1913657/1‑49 (MQ=255)
                   ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            >  2:3147884/1‑49 (MQ=255)
                   ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            >  2:5116390/1‑49 (MQ=255)
                   ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            >  1:4336439/1‑49 (MQ=255)
                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            <  2:2999720/48‑1 (MQ=255)
                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                          >  2:5325553/1‑50 (MQ=255)
                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGa                           <  1:1993488/49‑1 (MQ=255)
                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGa                           <  2:1400222/49‑1 (MQ=255)
                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:5662181/49‑1 (MQ=255)
                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:3454245/49‑1 (MQ=255)
                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        <  1:4149531/50‑1 (MQ=255)
                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  2:1798838/1‑50 (MQ=255)
                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       <  1:1673364/50‑1 (MQ=255)
                       aTAAACGTGCATGGCATGAGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  2:875094/1‑50 (MQ=39)
                         aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  2:614006/1‑50 (MQ=255)
                         aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:251408/1‑50 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     <  1:1172153/49‑1 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  2:1511160/50‑1 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  1:3892883/50‑1 (MQ=255)
                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  1:1547435/50‑1 (MQ=255)
                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    >  1:1689097/1‑49 (MQ=255)
                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTACa                   <  2:3938225/50‑1 (MQ=39)
                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  1:930415/1‑50 (MQ=255)
                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  1:372180/1‑50 (MQ=255)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  2:902789/50‑1 (MQ=255)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  1:1507079/50‑1 (MQ=255)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  1:2543497/50‑1 (MQ=255)
                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  2:1046373/50‑1 (MQ=255)
                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:768746/1‑49 (MQ=255)
                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  2:3546995/1‑49 (MQ=255)
                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  2:547722/50‑1 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               <  2:2999566/49‑1 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               <  1:1558153/49‑1 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               <  1:256131/49‑1 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  1:850231/1‑50 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt              >  2:4143298/1‑50 (MQ=255)
                                catggcatgCGGGAGCCTCTCAGTATCAGGAGCGCGAACGCTGCAATGAt              >  1:733115/1‑50 (MQ=255)
                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  1:2669148/1‑50 (MQ=255)
                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:1880042/1‑50 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          <  1:5243046/50‑1 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          <  2:2147672/50‑1 (MQ=255)
                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          <  2:5326459/50‑1 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  2:3835681/1‑49 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  2:3968667/1‑49 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt        <  2:2486215/50‑1 (MQ=255)
                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt        <  1:3508403/50‑1 (MQ=255)
                                        gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      >  2:3892074/1‑50 (MQ=255)
                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt     <  1:2074876/50‑1 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  2:3173657/1‑50 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  2:1706827/1‑50 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  1:2359477/1‑50 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  1:2270676/1‑50 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  2:1477901/1‑50 (MQ=255)
                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  1:1721213/1‑50 (MQ=255)
                                           ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   >  1:3864066/1‑50 (MQ=255)
                                           ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTgg   >  2:3984889/1‑50 (MQ=255)
                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  2:550092/50‑1 (MQ=255)
                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  2:2237483/50‑1 (MQ=255)
                                               |                                              
AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑