BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,562,085	C→T	A133T (GCA→ACA)	ddpA ←	putative D,D‑dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,562,085	0	C	T	100.0%	582.9 / NA	150	A133T (GCA→ACA)	ddpA	putative D,D‑dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (66/84); total (66/84)

Only 100 of 153 total aligned reads displayed.

CACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGT  >  NC_000913/1562038‑1562131

cACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGttt                                              >  2:677718/1‑50 (MQ=255)

cACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGttt                                              >  2:5134149/1‑50 (MQ=255)

 aCCGTATGTTCGTCGGGAGCATGAATCTTTAAATCTTTGGGAAATGTTTc                                             <  1:5226374/50‑1 (MQ=255)

 aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             <  2:1384369/50‑1 (MQ=255)

 aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             <  2:1285012/50‑1 (MQ=255)

 aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             >  1:4379042/1‑50 (MQ=255)

 aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             >  1:3292338/1‑50 (MQ=255)

  ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                            <  1:4801063/50‑1 (MQ=255)

  ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                            <  1:4685087/50‑1 (MQ=255)

  ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                            >  1:4377172/1‑50 (MQ=255)

   cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctg                                           <  2:1575196/50‑1 (MQ=255)

   cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctg                                           <  1:1941404/50‑1 (MQ=255)

    gTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgc                                          >  1:5083843/1‑50 (MQ=255)

      aTGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAGATGTTTctgctg                                        >  1:1137215/1‑50 (MQ=255)

      aTGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg                                        >  1:5543157/1‑50 (MQ=255)

       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg                                        <  2:4911870/49‑1 (MQ=255)

       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg                                        <  1:611077/49‑1 (MQ=255)

       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTgg                                       >  1:4843865/1‑50 (MQ=255)

       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTgg                                       >  1:502242/1‑50 (MQ=255)

       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTgg                                       <  1:3597970/50‑1 (MQ=255)

       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTgg                                       <  1:705340/50‑1 (MQ=255)

        gTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGc                                      >  2:273303/1‑50 (MQ=255)

         ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcc                                     <  2:4132451/50‑1 (MQ=255)

         ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGc                                      <  2:83930/49‑1 (MQ=255)

         ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGc                                      <  2:388487/49‑1 (MQ=255)

          tcgtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGccc                                    >  2:1209274/1‑50 (MQ=255)

            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  <  2:4076098/50‑1 (MQ=255)

            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  <  1:1657957/50‑1 (MQ=255)

            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  >  2:4255864/1‑50 (MQ=255)

            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  <  1:5121220/50‑1 (MQ=255)

            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  >  1:1663864/1‑50 (MQ=255)

            gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  <  1:252462/50‑1 (MQ=255)

               gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTgg                                <  2:3297375/49‑1 (MQ=255)

               gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGc                               <  1:1822936/50‑1 (MQ=255)

               gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGc                               >  2:2233618/1‑50 (MQ=255)

               gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGc                               >  2:753480/1‑50 (MQ=255)

                ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              <  1:1884307/50‑1 (MQ=255)

                ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              <  2:3902576/50‑1 (MQ=255)

                 gAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              >  2:4385968/1‑49 (MQ=255)

                  aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            >  1:2103626/1‑50 (MQ=255)

                  aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            >  2:4400946/1‑50 (MQ=255)

                  aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            >  2:1908483/1‑50 (MQ=255)

                  aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            >  2:2938878/1‑50 (MQ=255)

                  aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            <  2:4507897/50‑1 (MQ=255)

                    cATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                          <  1:2675273/50‑1 (MQ=255)

                    cATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                          >  2:1546000/1‑50 (MQ=255)

                     aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttt                         >  2:120497/1‑50 (MQ=255)

                     aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttt                         >  2:5631713/1‑50 (MQ=255)

                     aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttt                         >  2:2447827/1‑50 (MQ=255)

                      tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  2:3726887/1‑50 (MQ=255)

                      tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        <  1:2784268/50‑1 (MQ=255)

                      tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        <  1:445988/50‑1 (MQ=255)

                      tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        <  2:2525590/50‑1 (MQ=255)

                       cAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  2:2905565/1‑49 (MQ=255)

                       cAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  1:3806911/1‑49 (MQ=255)

                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttttt                       <  1:1462839/49‑1 (MQ=255)

                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  2:3598141/1‑48 (MQ=255)

                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTta                      <  2:897691/50‑1 (MQ=255)

                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTta                      <  2:4653932/50‑1 (MQ=255)

                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTta                      <  2:1986997/50‑1 (MQ=255)

                         aTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                     >  1:4763163/1‑50 (MQ=255)

                         aTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                     <  1:551665/50‑1 (MQ=255)

                          tCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttttt                       <  2:1469518/47‑1 (MQ=255)

                            tttAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                  >  2:2640960/1‑50 (MQ=255)

                              tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                  <  2:5218886/48‑1 (MQ=255)

                              tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                  <  1:5496099/48‑1 (MQ=255)

                              tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagt                    <  1:1444357/46‑1 (MQ=255)

                              tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc                >  2:2024762/1‑50 (MQ=255)

                              tAAATCTTTAGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagta                   <  1:1115566/47‑1 (MQ=39)

                               aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc                <  1:730719/49‑1 (MQ=255)

                               aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc                <  1:867368/49‑1 (MQ=255)

                               aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc                <  1:1323325/49‑1 (MQ=255)

                               aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCg               >  1:5550903/1‑50 (MQ=255)

                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCgt              >  2:3474122/1‑49 (MQ=255)

                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCg               <  1:3784830/49‑1 (MQ=255)

                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGc              >  2:339736/1‑50 (MQ=255)

                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGc              >  1:4608285/1‑50 (MQ=255)

                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGc              >  1:2354951/1‑50 (MQ=255)

                                 aTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCt             <  2:191050/50‑1 (MQ=255)

                                 aTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCt             <  2:4615249/50‑1 (MQ=255)

                                    tttGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa           >  1:5402318/1‑49 (MQ=255)

                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc            <  2:3324142/46‑1 (MQ=255)

                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc            <  1:3640410/46‑1 (MQ=255)

                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc            <  1:3907909/46‑1 (MQ=255)

                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaaa         <  1:5312458/49‑1 (MQ=255)

                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaaa         <  2:4687409/49‑1 (MQ=255)

                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          <  2:4961699/48‑1 (MQ=255)

                                      tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          <  1:1316956/48‑1 (MQ=255)

                                      tGGGAAACGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaaa         <  2:665527/49‑1 (MQ=255)

                                       gggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaaaa        <  2:2851984/49‑1 (MQ=255)

                                        ggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      <  2:419816/50‑1 (MQ=255)

                                         gAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaa     <  1:1992339/50‑1 (MQ=255)

                                         gAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaa     <  1:5210972/50‑1 (MQ=255)

                                         gAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      >  1:3861488/1‑49 (MQ=255)

                                         gAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      >  2:5123581/1‑49 (MQ=255)

                                          aaaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      >  1:2515702/1‑48 (MQ=255)

                                          aaaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      >  2:5441839/1‑48 (MQ=255)

                                           aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaag   >  2:5209740/1‑50 (MQ=255)

                                           aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaag   >  2:4571494/1‑50 (MQ=255)

                                            aTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGt  <  2:2243960/50‑1 (MQ=255)

CACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGT  >  NC_000913/1562038‑1562131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`