Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 214,885 C→T T18I (ACC→ATC)  arfB → peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913214,8850CT100.0% 473.4 / NA 121T18I (ACC→ATC) arfBpeptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (60/61);  total (60/61)

Only 100 of 125 total aligned reads displayed.

TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214838‑214931
                                               |                                              
tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                              >  2:489264/1‑50 (MQ=255)
 gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:5455881/1‑50 (MQ=255)
   gATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa                                           >  1:4976867/1‑50 (MQ=255)
   gATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa                                           >  2:2811216/1‑50 (MQ=255)
   gATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa                                           >  1:5602230/1‑50 (MQ=255)
    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa                                           <  2:3805681/49‑1 (MQ=255)
    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          <  1:4506202/50‑1 (MQ=255)
    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          >  2:5535122/1‑50 (MQ=255)
    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          <  2:3646862/50‑1 (MQ=255)
    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          >  1:3149883/1‑50 (MQ=255)
    aTTTCCCGACATGTTGCTATCCCCGATGGTGAGCTTGAGATCATCGCCa                                           <  1:2728429/49‑1 (MQ=39)
      ttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        >  1:979467/1‑50 (MQ=255)
       tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        <  2:5599655/49‑1 (MQ=255)
       tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        <  1:4862535/49‑1 (MQ=255)
       tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       >  1:1675093/1‑50 (MQ=255)
        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       <  1:4599487/49‑1 (MQ=255)
        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       <  2:549750/49‑1 (MQ=255)
        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       <  2:1681192/49‑1 (MQ=255)
         ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     <  1:878917/50‑1 (MQ=255)
         ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     >  2:3222227/1‑50 (MQ=255)
          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  2:4802985/50‑1 (MQ=255)
          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  2:2908899/50‑1 (MQ=255)
          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  1:3821084/50‑1 (MQ=255)
           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   <  2:405656/50‑1 (MQ=255)
           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:1349059/1‑50 (MQ=255)
            aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  2:4234624/1‑49 (MQ=255)
            aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  2:3314067/1‑49 (MQ=255)
            aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  2:312844/1‑49 (MQ=255)
             cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:5558555/1‑48 (MQ=255)
             cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:3648173/1‑48 (MQ=255)
             cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:3028389/1‑48 (MQ=255)
              aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                >  1:5074244/1‑50 (MQ=255)
               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  2:1242933/50‑1 (MQ=255)
               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  2:1640021/50‑1 (MQ=255)
               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  1:2347191/1‑50 (MQ=255)
               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  1:4452338/50‑1 (MQ=255)
               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  2:3978511/50‑1 (MQ=255)
               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  2:3942720/1‑50 (MQ=255)
                gTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg                              >  2:5217534/1‑50 (MQ=255)
                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             <  1:3816721/50‑1 (MQ=255)
                   gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           >  1:1655534/1‑50 (MQ=255)
                   gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           >  2:1793177/1‑50 (MQ=255)
                   gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcg                            <  1:4510530/49‑1 (MQ=255)
                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           <  1:3974138/49‑1 (MQ=255)
                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           <  1:4111643/49‑1 (MQ=255)
                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           <  1:190385/49‑1 (MQ=255)
                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                          >  1:4851097/1‑50 (MQ=255)
                     tATTCCCGATTGTTAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  2:2408666/1‑50 (MQ=37)
                     tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  2:2531259/1‑50 (MQ=255)
                     tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  1:5381052/1‑50 (MQ=255)
                     tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  2:4198232/1‑50 (MQ=255)
                      aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        >  1:5437030/1‑50 (MQ=255)
                       ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       <  1:450552/50‑1 (MQ=255)
                        tCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcg                      >  2:105157/1‑50 (MQ=255)
                         cccGATGGTGAGCTTTAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     >  2:2332001/1‑50 (MQ=38)
                         cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     >  1:5359822/1‑50 (MQ=255)
                          ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     <  1:1715722/49‑1 (MQ=255)
                          ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     <  1:4854983/49‑1 (MQ=255)
                          ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    <  2:782984/50‑1 (MQ=255)
                             aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  2:1506061/1‑50 (MQ=255)
                             aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  2:3917434/1‑50 (MQ=255)
                             aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 <  1:3784520/50‑1 (MQ=255)
                              tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  1:2138760/1‑49 (MQ=255)
                              tGGTGAGCTTGAGATCATCGCCATTCGAGCGCAGGGCGCGGGCGGgcag                 >  2:5428826/1‑49 (MQ=38)
                                gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca               <  2:2896653/49‑1 (MQ=255)
                                gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt              >  1:2567021/1‑50 (MQ=255)
                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  2:2176786/50‑1 (MQ=255)
                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             >  1:5643503/1‑50 (MQ=255)
                                  gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            >  1:1903399/1‑50 (MQ=255)
                                  gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCAGGCGGGCAGCATGt            >  2:3989594/1‑50 (MQ=38)
                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt           <  1:2796219/50‑1 (MQ=255)
                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt           <  2:3708872/50‑1 (MQ=255)
                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            <  2:4967258/49‑1 (MQ=255)
                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            <  1:1351603/49‑1 (MQ=255)
                                    gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt           >  2:1500006/1‑49 (MQ=255)
                                    gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  1:3464551/50‑1 (MQ=255)
                                    gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  2:4678358/50‑1 (MQ=255)
                                    gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  1:3216965/50‑1 (MQ=255)
                                     cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  2:3138849/1‑50 (MQ=255)
                                     cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:3046545/1‑50 (MQ=255)
                                     cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         <  2:2368435/50‑1 (MQ=255)
                                      ttGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:1891528/1‑49 (MQ=255)
                                      ttGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:3753906/1‑49 (MQ=255)
                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata       <  1:3967458/49‑1 (MQ=255)
                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata       <  2:4452428/49‑1 (MQ=255)
                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        <  2:2753081/48‑1 (MQ=255)
                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        >  2:470089/1‑48 (MQ=255)
                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa      <  2:2049210/49‑1 (MQ=255)
                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa      <  2:1991795/49‑1 (MQ=255)
                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg     <  1:1622279/50‑1 (MQ=255)
                                          gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa    <  1:557398/50‑1 (MQ=255)
                                          gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa    >  1:1292164/1‑50 (MQ=255)
                                          gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa    <  1:4120261/50‑1 (MQ=255)
                                          gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa    <  1:3586097/50‑1 (MQ=255)
                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   >  1:1211480/1‑50 (MQ=255)
                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   <  2:4289924/50‑1 (MQ=255)
                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   >  1:4986451/1‑50 (MQ=255)
                                            tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  <  1:3399015/50‑1 (MQ=255)
                                            tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  >  1:5054824/1‑50 (MQ=255)
                                            tCATCGCCATTCGTGAGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  >  2:3827558/1‑50 (MQ=38)
                                               |                                              
TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214838‑214931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑