| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,662,004 | T→C | E43G (GAA→GGA) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,662,004 | 0 | T | C | 96.5% | 539.1 / 6.5 | 144 | E43G (GAA→GGA) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (73/65); total (77/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
GTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAA > NC_000913/2661957‑2662050 | gTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTccc < 1:4351000/50‑1 (MQ=255) tACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCa < 1:1192626/50‑1 (MQ=255) tACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa < 1:1780199/50‑1 (MQ=255) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa < 1:374324/50‑1 (MQ=255) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAg < 2:1748527/49‑1 (MQ=255) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAg < 2:2352720/49‑1 (MQ=255) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAg < 2:4578668/49‑1 (MQ=255) gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt < 1:574618/50‑1 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt > 2:2541622/1‑49 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 1:1237630/1‑49 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 1:4368995/1‑49 (MQ=255) tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 2:3816580/1‑48 (MQ=255) tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 2:1953350/1‑48 (MQ=255) tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 2:2785223/1‑48 (MQ=255) ggAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 1:2020588/1‑47 (MQ=255) ggAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 2:1239838/1‑47 (MQ=255) gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATaa < 2:742144/48‑1 (MQ=255) gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATaa < 1:3431290/48‑1 (MQ=255) gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg > 1:1362634/1‑49 (MQ=255) aaaCCAGACCATTTTTACGCAGCCGGGACAACAGTTGTACCAGATAAGa > 2:1822067/1‑49 (MQ=38) aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa < 1:5488754/50‑1 (MQ=255) aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa < 2:3228191/50‑1 (MQ=255) aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAg < 1:3202644/50‑1 (MQ=255) aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAg < 2:4034893/50‑1 (MQ=255) aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAg > 1:1680365/1‑50 (MQ=255) ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAgg > 1:1071828/1‑50 (MQ=255) ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg > 1:1033414/1‑50 (MQ=255) ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg < 1:1601248/50‑1 (MQ=255) ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg > 2:2709035/1‑50 (MQ=255) cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAggg < 2:823725/50‑1 (MQ=255) cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg > 1:4164013/1‑49 (MQ=255) cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg > 2:2650693/1‑49 (MQ=255) aGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAggg > 2:1201971/1‑49 (MQ=255) gACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaa < 2:5532062/50‑1 (MQ=255) gACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGa > 2:2872401/1‑49 (MQ=255) aCCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaaa < 1:4659069/50‑1 (MQ=255) cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt < 1:339324/50‑1 (MQ=255) cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt > 2:4373841/1‑50 (MQ=255) tttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTcc > 2:5040308/1‑50 (MQ=255) ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc > 2:3157703/1‑50 (MQ=255) ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc > 1:2967045/1‑50 (MQ=255) tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc < 2:2058358/49‑1 (MQ=255) tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc < 1:2503537/49‑1 (MQ=255) tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc < 2:5501806/49‑1 (MQ=255) ttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt < 1:4965083/49‑1 (MQ=255) ttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt < 1:4829211/49‑1 (MQ=255) ttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt < 1:2498883/49‑1 (MQ=255) ttACGCAGACGGGAAAACAGTTGGCCCAGATAAGAAAGGGAAATTCCCt < 1:5108983/49‑1 (MQ=39) tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGa > 2:2953163/1‑50 (MQ=255) tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGa > 2:425521/1‑50 (MQ=255) aCGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGa < 1:3196545/49‑1 (MQ=255) aCGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc > 2:1344533/1‑50 (MQ=255) cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc < 2:3596706/49‑1 (MQ=255) ggaGACGGGCAACCCGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt > 2:5008122/3‑50 (MQ=255) gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt < 1:5392700/50‑1 (MQ=255) gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt > 2:1850832/1‑50 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGCTAAGAAAGGGAAATTCCCTGACGtt > 2:879793/1‑50 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt < 1:4159673/50‑1 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt > 1:220291/1‑50 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt < 1:2318626/50‑1 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt < 1:5017309/50‑1 (MQ=255) aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc < 1:796035/50‑1 (MQ=255) aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc < 2:71747/50‑1 (MQ=255) aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc < 2:3134763/50‑1 (MQ=255) aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc > 2:2553716/1‑50 (MQ=255) aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc < 2:1110911/50‑1 (MQ=255) gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg < 1:2839864/50‑1 (MQ=255) gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg > 1:3727003/1‑50 (MQ=255) cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg > 2:4091669/1‑49 (MQ=255) cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg > 1:1926464/1‑49 (MQ=255) cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa < 1:477747/50‑1 (MQ=255) cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa < 2:1408620/50‑1 (MQ=255) gggAAAGCAGATGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa > 2:3794261/1‑49 (MQ=255) gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa > 1:4524692/1‑49 (MQ=255) ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa > 2:1560720/1‑50 (MQ=255) ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa < 2:1040528/50‑1 (MQ=255) ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa > 1:4815068/1‑50 (MQ=255) gAAAACAGTTGTTCCAGATAAGAAAGGGAAATTACCTGACGTTCGGaaa > 2:975280/1‑49 (MQ=255) gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa > 2:2408036/1‑49 (MQ=255) gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa > 1:5166601/1‑49 (MQ=255) aaaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata < 2:4300956/50‑1 (MQ=255) aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 2:4445131/1‑50 (MQ=255) aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 2:673543/1‑50 (MQ=255) aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 2:2276179/1‑49 (MQ=255) aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 2:3863029/1‑49 (MQ=255) aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc < 2:1595183/50‑1 (MQ=255) aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 2:1009145/1‑48 (MQ=255) aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa < 1:4389071/50‑1 (MQ=255) aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa < 1:1314586/50‑1 (MQ=255) cAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg < 1:1167443/50‑1 (MQ=255) cAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg > 2:5327264/1‑50 (MQ=255) aGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc > 1:4956457/1‑50 (MQ=255) gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc < 2:3559780/50‑1 (MQ=255) gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc > 2:3965992/1‑49 (MQ=255) ggtgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc > 2:3995214/3‑49 (MQ=255) ttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc > 2:5492055/1‑49 (MQ=255) ttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc > 2:5552794/1‑49 (MQ=255) tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa > 1:3061683/1‑50 (MQ=255) tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa > 1:32250/1‑50 (MQ=255) tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa > 2:1444093/1‑50 (MQ=255) | GTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAA > NC_000913/2661957‑2662050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |