Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 257,794 T→C intergenic (+23/‑35) frsA → / → crl fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913257,7940TC100.0% 660.6 / NA 157intergenic (+23/‑35)frsA/crlfermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (84/73);  total (84/73)

Only 100 of 157 total aligned reads displayed.

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840
                                               |                                              
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:4857602/50‑1 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:1822380/50‑1 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              >  2:38453/1‑50 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:5025888/50‑1 (MQ=255)
cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  2:1203381/50‑1 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:4340172/1‑50 (MQ=255)
 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  2:3610620/1‑50 (MQ=255)
  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  2:706528/1‑49 (MQ=255)
  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  2:4359042/1‑49 (MQ=255)
  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  2:1003381/1‑49 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                            <  1:2528752/49‑1 (MQ=255)
   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           <  2:2154781/50‑1 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:4122871/1‑49 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:2191823/1‑49 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  2:3834536/1‑49 (MQ=255)
    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  2:5485567/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  2:863087/50‑1 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          >  2:1445683/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          >  2:3227539/1‑49 (MQ=255)
     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          >  1:1332085/1‑49 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:2142449/1‑50 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:786678/1‑50 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:1889107/1‑50 (MQ=255)
      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:3977806/1‑50 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:1310302/1‑49 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:2616510/1‑49 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        <  2:482026/49‑1 (MQ=255)
       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  2:3384147/50‑1 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  2:4216982/1‑48 (MQ=255)
        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa                                      <  2:937246/50‑1 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  2:4913378/1‑50 (MQ=255)
          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  1:674440/1‑50 (MQ=255)
           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   >  2:476599/1‑50 (MQ=255)
           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   >  1:3733063/1‑50 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  2:4379420/1‑50 (MQ=255)
            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  1:2217240/1‑50 (MQ=255)
               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               <  1:5194714/50‑1 (MQ=255)
                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  2:1436949/1‑49 (MQ=255)
                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  2:2782979/1‑50 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:4019902/1‑49 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             <  2:2418480/49‑1 (MQ=255)
                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                              <  1:927050/48‑1 (MQ=255)
                    gtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:1740693/1‑50 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                         <  1:2085143/50‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          <  2:3633342/49‑1 (MQ=255)
                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          <  1:2114027/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:1572300/49‑1 (MQ=255)
                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:4155327/1‑50 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAgg                      <  1:4177140/50‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:5250460/49‑1 (MQ=255)
                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:3998061/1‑49 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:3130744/1‑48 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:3276827/1‑48 (MQ=255)
                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:3980109/1‑48 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:2217158/1‑47 (MQ=255)
                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                    <  2:340331/50‑1 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:4804509/1‑46 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  2:3179982/1‑46 (MQ=255)
                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   <  1:3368564/50‑1 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  2:5590190/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:4400106/1‑49 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt                  <  2:93356/50‑1 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt                  <  2:583265/50‑1 (MQ=255)
                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt                  <  2:3119033/50‑1 (MQ=255)
                               gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:420305/1‑49 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATatc               <  2:3030862/49‑3 (MQ=39)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:3340121/49‑1 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:2479377/49‑1 (MQ=255)
                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCa              <  2:56058/50‑1 (MQ=255)
                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             >  2:628593/1‑50 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             <  2:2707131/48‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             <  1:5529218/48‑1 (MQ=255)
                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  2:1769958/49‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:747649/1‑50 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          <  2:1632557/50‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          <  2:1367524/50‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          <  2:477406/50‑1 (MQ=255)
                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:2646132/1‑50 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         <  2:2463216/50‑1 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         <  1:948209/50‑1 (MQ=255)
                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         >  2:2535351/1‑50 (MQ=255)
                                      tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        >  1:977197/1‑50 (MQ=255)
                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       <  1:1444287/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  1:3039144/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  2:3183893/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  2:5196845/49‑1 (MQ=255)
                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  2:5638058/49‑1 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     <  1:307138/49‑1 (MQ=255)
                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    >  1:212988/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   <  1:4428438/50‑1 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:1080359/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:535607/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:3594852/1‑50 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   <  1:4053154/50‑1 (MQ=255)
                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   <  1:2886462/50‑1 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATTACGTTAcc   >  1:3461771/1‑49 (MQ=38)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  2:2076473/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  2:5362401/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  2:1982555/1‑49 (MQ=255)
                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:3855328/50‑1 (MQ=255)
                                               |                                              
CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑