Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,477,150 C→T K90K (AAG→AAA)  fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,1500CT100.0% 719.0 / NA 178K90K (AAG→AAA) fkpApeptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (93/85);  total (93/85)

Only 100 of 180 total aligned reads displayed.

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196
                                              |                                              
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  2:4239586/49‑1 (MQ=255)
cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             >  2:712237/1‑50 (MQ=255)
 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:5046577/49‑1 (MQ=255)
 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  2:2013522/1‑50 (MQ=255)
 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:219261/1‑50 (MQ=255)
  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                           <  1:3931400/50‑1 (MQ=255)
   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  2:1667461/50‑1 (MQ=255)
    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         <  2:1754332/50‑1 (MQ=255)
    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         <  2:4561266/50‑1 (MQ=255)
     gCTTGTAGAGTCTGTTCTATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:3839183/1‑49 (MQ=255)
     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:5324914/1‑49 (MQ=255)
     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  2:5090233/1‑49 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  2:846726/49‑1 (MQ=255)
      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:2468596/49‑1 (MQ=255)
       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:2851675/1‑49 (MQ=255)
        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  2:3956066/1‑50 (MQ=255)
        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:4486448/1‑50 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:1333702/49‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  2:1035168/49‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  2:5294679/49‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:4471680/48‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  2:623321/48‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  2:3123625/48‑1 (MQ=255)
         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    >  2:5650241/1‑50 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:1619600/49‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:5435534/49‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  2:4000065/49‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  2:1578056/50‑1 (MQ=255)
          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGAAAAt                                    <  2:4490822/49‑1 (MQ=38)
           agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  2:1177673/1‑49 (MQ=255)
           agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  1:2563059/1‑49 (MQ=255)
            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  2:4805654/49‑1 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:107050/1‑50 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:2818790/1‑50 (MQ=255)
              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               <  1:1779103/50‑1 (MQ=255)
                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  2:4424802/1‑49 (MQ=255)
                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  2:5140840/1‑49 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  2:980833/47‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:1285665/48‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  2:4403041/48‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:4301851/48‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:5684960/48‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                           <  1:5021479/49‑1 (MQ=255)
                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                           <  1:2506450/49‑1 (MQ=255)
                    tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:1231396/1‑49 (MQ=255)
                    tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:3151558/1‑49 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  2:767265/1‑50 (MQ=255)
                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  2:3788741/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:2982969/1‑50 (MQ=255)
                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        <  1:3426354/49‑1 (MQ=255)
                       aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:4097860/1‑49 (MQ=255)
                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:746713/1‑48 (MQ=255)
                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  2:5551995/1‑48 (MQ=255)
                         ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    >  2:5197157/1‑50 (MQ=255)
                         ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    >  1:1458726/1‑50 (MQ=255)
                         ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    >  1:2062524/1‑50 (MQ=255)
                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGACCACCAGc                   >  2:1637324/1‑50 (MQ=255)
                           ctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:4915862/1‑49 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:1009599/45‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:795290/45‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  2:1213123/46‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:155129/46‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  2:3540234/47‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  2:2829503/48‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:183408/48‑1 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 >  1:1420076/1‑50 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 >  1:355662/1‑50 (MQ=255)
                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 >  1:2057306/1‑50 (MQ=255)
                             tGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  2:2633627/1‑50 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 <  2:1316440/48‑1 (MQ=255)
                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                <  1:893078/49‑1 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  2:3454752/1‑48 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  2:419558/1‑48 (MQ=255)
                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  2:3608224/49‑1 (MQ=255)
                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             <  1:3503001/50‑1 (MQ=255)
                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             <  1:2958908/50‑1 (MQ=255)
                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  2:2393768/1‑50 (MQ=255)
                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  2:11409/1‑50 (MQ=255)
                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  2:3827179/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  2:1822928/49‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  2:3327585/49‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  2:1405358/50‑1 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:4071570/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:4518120/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:165734/1‑50 (MQ=255)
                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:372634/1‑50 (MQ=255)
                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:3510706/50‑1 (MQ=255)
                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  2:1435208/50‑1 (MQ=255)
                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         >  1:4748028/1‑50 (MQ=255)
                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        >  2:1882300/1‑50 (MQ=255)
                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        <  2:5421481/50‑1 (MQ=255)
                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        <  2:3976543/50‑1 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  2:5440854/1‑50 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:494570/50‑1 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:614875/1‑50 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:2410122/1‑50 (MQ=255)
                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:4226733/1‑50 (MQ=255)
                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     >  1:275709/1‑50 (MQ=255)
                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCGGCTGATCttt    <  1:4940397/49‑1 (MQ=39)
                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:4634244/1‑50 (MQ=255)
                                              |                                              
CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑