Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,513,003 A→G D212D (GAT→GAC)  trpS ← tryptophan‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,513,0030AG100.0% 606.3 / NA 150D212D (GAT→GAC) trpStryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (89/61);  total (89/61)

Only 100 of 150 total aligned reads displayed.

TCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512956‑3513048
                                               |                                             
tcgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                             <  2:2303672/50‑1 (MQ=255)
tcgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                             >  2:3178323/1‑50 (MQ=255)
 cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:344474/1‑50 (MQ=255)
 cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  2:1027317/1‑50 (MQ=255)
  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  2:1258261/1‑50 (MQ=255)
  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           <  1:3941875/50‑1 (MQ=255)
  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           <  1:4726852/50‑1 (MQ=255)
  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            <  1:5355185/49‑1 (MQ=255)
   gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          <  2:2805523/50‑1 (MQ=255)
   gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:4246777/1‑50 (MQ=255)
    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  2:5286743/50‑1 (MQ=255)
    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:2645697/50‑1 (MQ=255)
     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  2:2607545/1‑48 (MQ=255)
     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:5651069/1‑48 (MQ=255)
     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  2:3243648/1‑48 (MQ=255)
      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  2:4012416/1‑50 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  2:1552310/50‑1 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:4120406/50‑1 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:703088/49‑1 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:1863317/49‑1 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  2:5226713/1‑48 (MQ=255)
        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:4052574/1‑48 (MQ=255)
         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:5220853/1‑50 (MQ=255)
         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    <  2:4318638/50‑1 (MQ=255)
          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  2:4298962/50‑1 (MQ=255)
          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  2:653665/1‑50 (MQ=255)
           gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  2:3550802/1‑50 (MQ=255)
            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1199526/1‑50 (MQ=255)
            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:4626097/50‑1 (MQ=255)
            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  <  2:3448947/49‑1 (MQ=255)
             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1607975/1‑49 (MQ=255)
             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCg                                <  1:1792977/50‑1 (MQ=255)
                cACGTTTGGTTTTCTTCACTACCGATTTCGGGTCTCCCAGCAGGCCGAt                              >  2:3604508/1‑49 (MQ=38)
                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  2:4916854/1‑49 (MQ=255)
                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  2:517209/1‑49 (MQ=255)
                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:5073327/1‑49 (MQ=255)
                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  2:3839594/1‑49 (MQ=255)
                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  2:3217420/50‑1 (MQ=255)
                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  2:3810969/1‑50 (MQ=255)
                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  1:3238261/1‑50 (MQ=255)
                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:947726/50‑1 (MQ=255)
                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  2:4033011/1‑50 (MQ=255)
                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  2:2696828/50‑1 (MQ=255)
                  cGTTTGATTTTCTTCACTAACGATTTCGGGTCTTCCAGCAGGCCGATAAc                           >  2:1622792/1‑50 (MQ=38)
                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  2:3851471/1‑50 (MQ=255)
                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGtt                        <  2:5456801/49‑1 (MQ=255)
                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:1322896/1‑50 (MQ=255)
                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  2:829763/1‑50 (MQ=255)
                      tGATTTTCTTCACTACCGATTTCGGGTCTGCCAGCAGGCCGATAACGtt                        <  2:2348535/49‑1 (MQ=38)
                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  2:5411855/1‑50 (MQ=255)
                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  2:3697649/1‑50 (MQ=255)
                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  2:2369790/1‑50 (MQ=255)
                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:1033397/1‑50 (MQ=255)
                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    <  1:5162700/50‑1 (MQ=255)
                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    >  2:524495/1‑50 (MQ=255)
                          tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:5613738/1‑50 (MQ=255)
                          tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:2962340/1‑50 (MQ=255)
                           ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:1626028/1‑49 (MQ=255)
                            tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg                  <  1:3589172/49‑1 (MQ=255)
                            tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg                  <  1:989351/49‑1 (MQ=255)
                            tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg                  <  2:281789/49‑1 (MQ=255)
                            tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg                  <  2:4500723/49‑1 (MQ=255)
                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 <  2:5169012/49‑1 (MQ=255)
                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:647233/1‑50 (MQ=255)
                              ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:537568/1‑49 (MQ=255)
                               tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  2:2775013/1‑50 (MQ=255)
                               tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:3170324/1‑50 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:3256662/48‑1 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:1029246/48‑1 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                <  1:5455395/47‑1 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                <  2:652796/47‑1 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              <  2:842418/49‑1 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              <  2:1043529/49‑1 (MQ=255)
                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:272583/1‑50 (MQ=255)
                                cACTACCGATTTCGGGCCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:5301360/1‑50 (MQ=39)
                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:4159277/50‑1 (MQ=255)
                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            >  1:1142961/1‑50 (MQ=255)
                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            >  2:4203779/1‑50 (MQ=255)
                                  cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           <  2:1800662/50‑1 (MQ=255)
                                   tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  2:5229083/1‑50 (MQ=255)
                                   tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2550791/1‑50 (MQ=255)
                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2731114/1‑48 (MQ=255)
                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:4132476/49‑1 (MQ=255)
                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  2:5619560/1‑47 (MQ=255)
                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:230363/1‑47 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  2:121256/1‑50 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:4185591/1‑50 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  2:2369976/1‑50 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:5426932/1‑50 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  2:4914398/1‑50 (MQ=255)
                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:3179431/1‑50 (MQ=255)
                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     <  2:195760/50‑1 (MQ=255)
                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     <  2:608131/50‑1 (MQ=255)
                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:475472/1‑50 (MQ=255)
                                          ttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   >  1:1578587/1‑50 (MQ=255)
                                          ttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   >  1:4480328/1‑50 (MQ=255)
                                           tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   <  1:4639149/49‑1 (MQ=255)
                                           tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:2237304/1‑50 (MQ=255)
                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  2:2757654/49‑1 (MQ=255)
                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:2779929/49‑1 (MQ=255)
                                               |                                             
TCGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512956‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑