| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,760 | A→G | N431S (AAC→AGC) | igaA → | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,760 | 0 | A | G | 98.4% | 502.7 / ‑2.0 | 126 | N431S (AAC→AGC) | igaA | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (64/60); total (66/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-02 | |||||||||||
Only 100 of 141 total aligned reads displayed.
ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC > NC_000913/3527714‑3527803 | aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga > 2:2166600/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 2:4260593/50‑1 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 2:4061231/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 2:1596157/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 1:2579846/50‑1 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 1:1952720/50‑1 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:2219897/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:3322047/1‑50 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc < 2:5080927/50‑1 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc < 2:5074389/50‑1 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc > 2:4828883/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc > 1:5484756/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc < 1:1480060/50‑1 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc < 1:1108957/50‑1 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGcc > 1:4974265/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc > 1:2307802/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:4133916/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 2:5569854/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 2:159837/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 2:4190934/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 2:4330284/50‑1 (MQ=255) cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg < 2:1104296/49‑1 (MQ=255) cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg < 2:4667349/49‑1 (MQ=255) cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGc > 2:1976562/1‑50 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc < 1:2598654/49‑1 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 2:1947958/1‑50 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt < 2:2011569/50‑1 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:3638953/1‑49 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc < 1:4458797/50‑1 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa > 2:444629/1‑50 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa > 2:2537769/1‑50 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa < 2:3076132/50‑1 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 2:2444581/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 1:1641390/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 2:2421895/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt < 1:676399/49‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt < 2:169780/49‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt < 2:4512843/49‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt < 2:2283211/49‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt < 2:3754336/48‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg > 1:1070173/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg > 2:401799/1‑50 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg < 2:3512842/49‑1 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg < 1:3176683/49‑1 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 2:4428013/1‑50 (MQ=255) ttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc < 1:5473075/49‑1 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGcc < 2:3336593/49‑1 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGcc < 1:3625035/49‑1 (MQ=255) tACCGTTTGACGGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGcc < 1:4912546/49‑1 (MQ=39) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa < 1:23537/49‑1 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAtt < 2:5556917/50‑1 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 2:2884909/1‑49 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:2792230/1‑49 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa > 1:128517/1‑50 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa > 2:3889393/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 2:2479974/50‑1 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 2:733289/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 1:3320/50‑1 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 1:1278096/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAcc < 1:606949/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAcc < 1:3119088/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAcc < 1:2730027/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:5524386/1‑49 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:2662153/1‑49 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 2:2001829/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCa < 1:976360/49‑1 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCa < 1:4067510/49‑1 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg < 2:5427422/49‑1 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa < 2:5658953/50‑1 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 2:4940484/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 1:3171315/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 2:628490/1‑50 (MQ=255) tGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATc > 1:155798/1‑50 (MQ=255) gCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:4482255/1‑50 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:4664946/1‑49 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:3007246/1‑49 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:1964981/1‑49 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg < 2:2863351/50‑1 (MQ=255) cGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCATAATCTGaa > 1:4695113/1‑50 (MQ=39) cGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa > 2:3154285/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGa < 2:5435730/48‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc < 1:4511668/50‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 2:3392981/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc < 1:4725270/50‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:529667/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:5450230/1‑50 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt > 2:349181/1‑50 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt > 1:2866201/1‑50 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt < 1:2169697/50‑1 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt > 1:2112035/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 2:3434562/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:936499/1‑50 (MQ=255) atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt > 1:3836275/1‑50 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt > 1:4791848/1‑49 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc < 2:3979507/50‑1 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 2:4910114/1‑50 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca < 2:2891223/49‑1 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca < 2:2209564/49‑1 (MQ=255) tcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:1121450/1‑49 (MQ=255) cTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaac > 1:4580150/1‑49 (MQ=255) | ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC > NC_000913/3527714‑3527803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |