BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,891	G→A	Q68* (CAA→TAA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,891	0	G	A	100.0%	596.6 / NA	155	Q68* (CAA→TAA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (80/75); total (80/75)

Only 100 of 155 total aligned reads displayed.

GGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCA  >  NC_000913/3850844‑3850936

ggaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGc                                             >  2:3635116/1‑50 (MQ=255)

 gaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  2:2723718/1‑50 (MQ=255)

 gaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            <  2:1129125/50‑1 (MQ=255)

 gaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  2:1138426/1‑50 (MQ=255)

 gaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  1:5317014/1‑50 (MQ=255)

 gaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  1:2419176/1‑50 (MQ=255)

  aCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCtt                                           <  2:3812488/50‑1 (MQ=255)

  aCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCtt                                           <  1:4630493/50‑1 (MQ=255)

  aCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  1:1991997/1‑49 (MQ=255)

  aCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  1:3013922/1‑49 (MQ=255)

   cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTa                                          <  2:2516339/50‑1 (MQ=255)

   cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTa                                          <  2:4396525/50‑1 (MQ=255)

    tGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                         >  1:4175649/1‑50 (MQ=255)

     gATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                         >  1:4941441/1‑49 (MQ=255)

     gATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                         >  1:5052433/1‑49 (MQ=255)

      aTTGCGCTGCACGTTTACGACATCTTCCAGCTTATAGATTTAGCTTat                                         <  2:4529249/48‑1 (MQ=25)

      aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                       >  2:1774593/1‑50 (MQ=255)

      aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                       <  2:781162/50‑1 (MQ=255)

      aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                       <  1:1265804/50‑1 (MQ=255)

      aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatc                                        <  2:4837677/49‑1 (MQ=255)

      aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatc                                        <  2:1357952/49‑1 (MQ=255)

      aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                         <  2:2759297/48‑1 (MQ=255)

      aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                         <  2:5358489/48‑1 (MQ=255)

       ttACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcat                                      <  1:949771/50‑1 (MQ=255)

       ttACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcat                                      >  1:611460/1‑50 (MQ=255)

        tACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcatc                                     >  1:3946674/1‑50 (MQ=255)

         aCGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcatc                                     <  1:5372173/49‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                    <  1:1853508/49‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                    <  1:1308009/49‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                    <  1:4901568/49‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                    <  1:2658477/49‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                    <  2:4873262/49‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                    <  1:382437/49‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                   <  2:4767393/50‑1 (MQ=255)

          cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                   >  2:1207203/1‑50 (MQ=255)

             tGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                                >  2:4319926/1‑50 (MQ=255)

             tGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                                >  1:5679603/1‑50 (MQ=255)

              gCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                                >  1:2110520/1‑49 (MQ=255)

               cACTTTCACGTCATCTTCAAGCTTATCGATTTAGCTTATCATCTGCGCCa                              <  2:5527284/50‑1 (MQ=255)

               cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                                >  2:5081602/1‑48 (MQ=255)

               cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                                >  2:4081647/1‑48 (MQ=255)

               cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCa                              <  1:3730448/50‑1 (MQ=255)

               cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCa                              <  1:2910740/50‑1 (MQ=255)

               cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCa                              <  1:752127/50‑1 (MQ=255)

                aCTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                                >  2:697049/1‑47 (MQ=255)

                aCTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAg                             <  2:1642423/50‑1 (MQ=255)

                 cTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGa                            >  1:224392/1‑50 (MQ=255)

                   ttCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          >  2:1964221/1‑50 (MQ=255)

                   ttCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          >  2:3212589/1‑50 (MQ=255)

                   ttCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          >  2:4208182/1‑50 (MQ=255)

                   ttCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          >  2:3879551/1‑50 (MQ=255)

                   ttCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          >  1:3437194/1‑50 (MQ=255)

                    tCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGAc                           <  2:3454264/48‑1 (MQ=255)

                    tCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          <  2:3960387/49‑1 (MQ=255)

                    tCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGc                         >  1:2470911/1‑50 (MQ=255)

                      aCGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                       >  2:4048185/1‑50 (MQ=255)

                      aCGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                       <  1:3707622/50‑1 (MQ=255)

                      aCGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                       <  2:2213552/50‑1 (MQ=255)

                       cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTgg                      <  1:5597735/50‑1 (MQ=255)

                       cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTgg                      <  2:4182680/50‑1 (MQ=255)

                       cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTgg                      <  1:376203/50‑1 (MQ=255)

                        gACATGTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                     <  2:4906403/50‑1 (MQ=255)

                        gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                     <  2:1087835/50‑1 (MQ=255)

                        gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                     <  2:4721529/50‑1 (MQ=255)

                        gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                     >  1:119196/1‑50 (MQ=255)

                        gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                     >  1:843160/1‑50 (MQ=255)

                         aCATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtc                    <  2:1642931/50‑1 (MQ=255)

                         aCATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtc                    <  1:3412226/50‑1 (MQ=255)

                          cATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcg                   >  1:1000500/1‑50 (MQ=255)

                           aTCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcgt                  >  2:1857507/1‑50 (MQ=255)

                                ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcgtc                 <  2:1044195/46‑1 (MQ=255)

                                ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCa                <  1:3963957/47‑1 (MQ=255)

                                ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAtt              >  2:3042402/1‑49 (MQ=255)

                                ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAtt              >  2:4544486/1‑49 (MQ=255)

                                ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAt               <  2:4148655/48‑1 (MQ=255)

                                ccAGCTTATCGATTTAGCTTATCATCTGCCCCAGACGCTGGTCGTCAtt              <  2:4459518/49‑1 (MQ=255)

                                 cAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTg             <  1:4042904/49‑1 (MQ=255)

                                 cAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGa            >  1:768648/1‑50 (MQ=255)

                                  aGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc           >  2:2571582/1‑50 (MQ=255)

                                   gCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAcc          <  1:1481892/50‑1 (MQ=255)

                                   gCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc           >  1:2592069/1‑49 (MQ=255)

                                    cTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc           >  1:4394950/1‑48 (MQ=255)

                                    cTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCa         <  2:5316551/50‑1 (MQ=255)

                                     ttATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  1:1010393/1‑50 (MQ=255)

                                     ttATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  2:3049394/1‑50 (MQ=255)

                                     ttATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  1:454466/1‑50 (MQ=255)

                                      tATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  2:2093746/1‑49 (MQ=255)

                                      tATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  2:1456181/1‑49 (MQ=255)

                                       aTCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        <  1:394963/48‑1 (MQ=255)

                                        tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTa      <  1:1841905/49‑1 (MQ=255)

                                        tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAg     >  1:1460619/1‑50 (MQ=255)

                                         cGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  1:838692/1‑50 (MQ=255)

                                          gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGcc   <  1:3212153/50‑1 (MQ=255)

                                          gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  2:5013541/1‑49 (MQ=255)

                                          gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  1:4263273/1‑49 (MQ=255)

                                          gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  2:5644999/1‑49 (MQ=255)

                                          gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  2:680709/1‑49 (MQ=255)

                                           aTTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  2:1219774/1‑48 (MQ=255)

                                           aTTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCa  <  1:1206220/50‑1 (MQ=255)

                                            tttAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  1:632207/1‑47 (MQ=255)

GGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCA  >  NC_000913/3850844‑3850936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`