| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,926,760 | A→G | Y82H (TAC→CAC) | asnC ← | DNA‑binding transcriptional dual regulator AsnC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,926,760 | 0 | A | G | 75.3% | 301.5 / 113.5 | 154 | Y82H (TAC→CAC) | asnC | DNA‑binding transcriptional dual regulator AsnC |
| Reads supporting (aligned to +/- strand): ref base A (17/21); new base G (49/67); total (66/88) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
GCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCAT > NC_000913/3926711‑3926807 | gCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTa < 1:2237507/50‑1 (MQ=255) ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGggtggt > 1:253927/1‑50 (MQ=255) cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCt > 2:2979625/1‑50 (MQ=255) cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAACGCGGACGGGTAGt < 1:2642864/48‑1 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt > 2:3645788/1‑50 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt > 2:4915971/1‑50 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 1:2993692/50‑1 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 1:4701323/50‑1 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 1:5662481/50‑1 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt > 2:5552619/1‑50 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 2:1236181/50‑1 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCt < 2:3428755/49‑1 (MQ=255) aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCt < 2:503161/49‑1 (MQ=255) gTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCttt < 1:1602197/50‑1 (MQ=255) gTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCttt < 1:849154/50‑1 (MQ=255) gTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCttt < 2:4617932/50‑1 (MQ=255) tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTg < 1:248529/50‑1 (MQ=255) tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTg < 2:4571323/50‑1 (MQ=255) tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTg > 2:1894398/1‑50 (MQ=255) tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTg < 1:5320429/50‑1 (MQ=255) gACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTgg > 1:4999770/1‑50 (MQ=255) aCTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgc < 1:1954935/50‑1 (MQ=255) cTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcg < 1:4086062/50‑1 (MQ=255) cTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcg > 1:3765399/1‑50 (MQ=255) cTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcg > 1:2652558/1‑50 (MQ=255) ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc > 2:390051/1‑50 (MQ=255) ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc > 2:1116916/1‑50 (MQ=255) ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc > 1:4435779/1‑50 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc < 1:2299373/48‑1 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc > 1:1810631/1‑50 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc > 1:829254/1‑50 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc > 2:1988972/1‑50 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGct < 2:5168906/49‑1 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGct < 2:4249448/49‑1 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGct < 2:5405370/49‑1 (MQ=255) catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctc > 2:2522438/1‑50 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctct < 1:4496999/50‑1 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc < 2:4324336/49‑1 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc < 2:587465/49‑1 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctct < 2:4071443/50‑1 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctct < 1:64008/50‑1 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctc < 1:1055190/49‑1 (MQ=255) tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCtt > 2:1384482/1‑50 (MQ=255) tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCtt > 2:2059234/1‑50 (MQ=255) tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCtt < 1:22770/50‑1 (MQ=255) caAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCtt > 2:838210/1‑49 (MQ=255) caAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCtt > 2:933672/1‑49 (MQ=255) aaGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCttt < 2:2410586/49‑1 (MQ=255) aaGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTa < 1:3855137/50‑1 (MQ=255) aGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTaa > 1:4872862/1‑50 (MQ=255) aGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTaa < 1:3848534/50‑1 (MQ=255) aGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTaa < 1:2507354/50‑1 (MQ=255) ggCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTaa > 2:2312915/1‑49 (MQ=255) ggCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAat < 1:3565045/50‑1 (MQ=255) gCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAata < 2:3761070/50‑1 (MQ=255) cTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAatat < 1:1995588/50‑1 (MQ=255) ttCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATaa > 1:5525478/1‑50 (MQ=255) tCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATaa < 1:3714117/49‑1 (MQ=255) ccAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATg > 1:1141449/1‑50 (MQ=255) ccAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATg > 1:5210873/1‑50 (MQ=255) ccAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAAt < 1:2843053/49‑1 (MQ=255) cAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATg < 1:4234564/49‑1 (MQ=255) cAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGc > 1:5058941/1‑50 (MQ=255) aGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGcc > 2:2752763/1‑50 (MQ=255) aGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGcc < 1:4389508/50‑1 (MQ=255) gCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCg < 2:4093498/50‑1 (MQ=255) ttGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGAt > 1:672636/1‑49 (MQ=255) tGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:1659914/49‑1 (MQ=255) tGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:1147931/49‑1 (MQ=255) tGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:2435814/49‑1 (MQ=255) tGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:5157024/49‑1 (MQ=255) gCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATaa < 1:4712407/49‑1 (MQ=255) ccAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATaaa < 2:4768368/49‑1 (MQ=255) ccAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATaaa < 1:1553565/49‑1 (MQ=255) ccAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag < 2:5563168/50‑1 (MQ=255) cAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:1755880/1‑49 (MQ=255) cAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:866534/1‑49 (MQ=255) cAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:3733772/1‑49 (MQ=255) cAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:3547266/1‑49 (MQ=255) aGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:5694497/1‑48 (MQ=255) aGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:5380628/1‑48 (MQ=255) gcgcGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcag < 2:3339463/50‑1 (MQ=255) gcgcGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcag < 1:3649151/50‑1 (MQ=255) gcgcGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcag < 1:1405173/50‑1 (MQ=255) cgcgGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcagc < 2:5588957/50‑1 (MQ=255) gcgGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGcc < 1:1319315/50‑1 (MQ=255) ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa > 1:3184863/1‑50 (MQ=255) ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa > 1:1762392/1‑50 (MQ=255) ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa > 1:1714171/1‑50 (MQ=255) ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa < 1:5263008/50‑1 (MQ=255) ggAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa < 1:5228315/50‑1 (MQ=255) gAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTAc > 1:5628001/1‑50 (MQ=255) gAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTAc < 2:1609193/50‑1 (MQ=255) cgggTGGTCTTTGGCGCTCTTTAATATAATGCAGATAAAGCAGCCTACgg > 2:2053574/2‑49 (MQ=255) aGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGt > 2:1605714/1‑50 (MQ=255) gggTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGt > 2:5291142/1‑49 (MQ=255) gggTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTc < 2:1403541/50‑1 (MQ=255) gggTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGt > 2:1695170/1‑49 (MQ=255) gTAGTCTTTGGCGCTCTTTAATGTAATGCCGATAAAGCAGCCTACGTCAt < 1:3928155/50‑1 (MQ=255) tAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCAt > 1:954697/1‑49 (MQ=255) | GCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCAT > NC_000913/3926711‑3926807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |