Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,093,223 A→G V72V (GTT→GTC)  rhaM ← L‑rhamnose mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,093,2230AG100.0% 579.9 / NA 144V72V (GTT→GTC) rhaML‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (82/62);  total (82/62)

Only 100 of 144 total aligned reads displayed.

GGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093176‑4093269
                                               |                                              
gggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGAc                                              <  1:4349017/50‑1 (MQ=255)
gggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGAc                                              <  2:3387805/50‑1 (MQ=255)
gggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGAc                                              <  1:2796924/50‑1 (MQ=255)
 ggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACa                                             <  1:5562072/50‑1 (MQ=255)
  gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                            >  2:1097347/1‑50 (MQ=255)
  gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                            >  2:3622696/1‑50 (MQ=255)
   ttAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATc                                           <  2:4145600/50‑1 (MQ=255)
    tAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                            >  2:134037/1‑48 (MQ=255)
     aGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCa                                          <  2:1090714/49‑1 (MQ=255)
     aGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAg                                         >  2:5463909/1‑50 (MQ=255)
      gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAg                                         <  1:2185956/49‑1 (MQ=255)
      gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAg                                         <  1:2582173/49‑1 (MQ=255)
      gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                        <  2:246832/50‑1 (MQ=255)
       cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       <  2:5417266/50‑1 (MQ=255)
        gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                      >  1:1462845/1‑50 (MQ=255)
        gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                      >  2:726430/1‑50 (MQ=255)
        gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                      >  2:2054381/1‑50 (MQ=255)
         ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                     >  2:1516585/1‑50 (MQ=255)
         ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                     >  2:1743892/1‑50 (MQ=255)
          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    >  1:86403/1‑50 (MQ=255)
          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    >  1:3255280/1‑50 (MQ=255)
          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    <  1:1827402/50‑1 (MQ=255)
          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    <  1:145482/50‑1 (MQ=255)
          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    >  2:712818/1‑50 (MQ=255)
          gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    <  2:1275952/50‑1 (MQ=255)
           cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   <  2:3367372/50‑1 (MQ=255)
           cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  1:2876407/1‑50 (MQ=255)
           cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  2:3175985/1‑50 (MQ=255)
            aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  1:3978220/1‑49 (MQ=255)
            aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  1:4087214/1‑49 (MQ=255)
            aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  <  1:580492/50‑1 (MQ=255)
             tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  >  1:2831870/1‑49 (MQ=255)
             tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  >  1:2306287/1‑49 (MQ=255)
              aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa                                >  2:4934693/1‑50 (MQ=255)
              aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa                                >  2:4531874/1‑50 (MQ=255)
              aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa                                >  2:2852768/1‑50 (MQ=255)
               aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa                                <  1:4686994/49‑1 (MQ=255)
               aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc                               <  2:209974/50‑1 (MQ=255)
               aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc                               <  1:1048080/50‑1 (MQ=255)
                cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCCAcc                              <  2:2913991/50‑1 (MQ=255)
                cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc                              >  1:3193685/1‑50 (MQ=255)
                 aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  2:2884557/1‑50 (MQ=255)
                 aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:690522/1‑50 (MQ=255)
                 aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             <  1:741703/50‑1 (MQ=255)
                   cGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCa                           >  1:5531301/1‑50 (MQ=255)
                   cGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCa                           >  1:5612875/1‑50 (MQ=255)
                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          >  1:1356024/1‑50 (MQ=255)
                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          <  2:2319410/50‑1 (MQ=255)
                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          <  1:2648686/50‑1 (MQ=255)
                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          >  1:4263390/1‑50 (MQ=255)
                    ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          <  1:4921216/50‑1 (MQ=255)
                     gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                         >  1:4767114/1‑50 (MQ=255)
                     gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                         >  2:4031006/1‑50 (MQ=255)
                     gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                         <  1:5408018/50‑1 (MQ=255)
                      tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  2:470739/1‑50 (MQ=255)
                        atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc                       <  2:580249/49‑1 (MQ=255)
                        atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc                       <  2:861303/49‑1 (MQ=255)
                        atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc                       <  2:282234/49‑1 (MQ=255)
                        atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCa                      <  2:3701860/50‑1 (MQ=255)
                         tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg                     >  1:4059279/1‑50 (MQ=255)
                         tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg                     >  2:4318265/1‑50 (MQ=255)
                          ctatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    >  2:478128/2‑50 (MQ=255)
                          atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    >  1:1311263/1‑50 (MQ=255)
                           tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    <  2:3090021/49‑1 (MQ=255)
                           tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCg                   <  1:1013928/50‑1 (MQ=255)
                             tttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt                 >  1:1918204/1‑50 (MQ=255)
                             tttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt                 >  2:2931239/1‑50 (MQ=255)
                              ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc                >  1:4924264/1‑50 (MQ=255)
                              ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc                >  1:3574698/1‑50 (MQ=255)
                              ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc                >  1:3138895/1‑50 (MQ=255)
                              ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc                >  1:1039611/1‑50 (MQ=255)
                               tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttct               >  2:943074/1‑50 (MQ=255)
                               tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttct               >  2:1787453/1‑50 (MQ=255)
                                 caccaACGTTGGCAGACATCAGTGCTGGCAGCCGCATTCCAGCGttctt              <  2:4488695/49‑1 (MQ=255)
                                 caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttcttc             <  2:3738625/50‑1 (MQ=255)
                                 caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              <  2:2594554/49‑1 (MQ=255)
                                 caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:5249425/1‑49 (MQ=255)
                                  accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:4500277/1‑48 (MQ=255)
                                  accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCa            <  1:3558878/50‑1 (MQ=255)
                                   ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  2:2600547/1‑47 (MQ=255)
                                    caACGTTGGCAGACATCAGTGCTGGCGCCCGCATTCCAGCGTTCTTCAGa          <  2:4294416/50‑1 (MQ=255)
                                    caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:4795070/1‑46 (MQ=255)
                                     aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAt         >  1:195768/1‑50 (MQ=255)
                                     aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAt         >  1:3821317/1‑50 (MQ=255)
                                      aCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt        <  1:421330/50‑1 (MQ=255)
                                      aCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt        <  2:4670776/50‑1 (MQ=255)
                                       cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc       <  2:3112028/50‑1 (MQ=255)
                                       cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc       <  2:1475281/50‑1 (MQ=255)
                                        gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc       >  1:5486291/1‑49 (MQ=255)
                                        gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCa      <  2:2128764/50‑1 (MQ=255)
                                        gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCa      <  2:3256502/50‑1 (MQ=255)
                                         ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCaa     >  2:453992/1‑50 (MQ=255)
                                         ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCaa     >  1:3689737/1‑50 (MQ=255)
                                          tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt    >  2:5313325/1‑50 (MQ=255)
                                          tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt    >  2:5333579/1‑50 (MQ=255)
                                          tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt    >  1:866041/1‑50 (MQ=255)
                                           ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc   >  2:573409/1‑50 (MQ=255)
                                           ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt    <  2:5352583/49‑1 (MQ=255)
                                            gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct  <  2:1208042/50‑1 (MQ=255)
                                            gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct  <  1:2775948/50‑1 (MQ=255)
                                               |                                              
GGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093176‑4093269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑