BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	643.3 / NA	161	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (74/87); total (74/87)

Only 100 of 161 total aligned reads displayed.

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  2:3957275/1‑50 (MQ=255)

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  2:399152/1‑50 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             <  1:2328718/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                            <  2:1654200/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  2:2663734/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:5554473/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           <  1:5217547/50‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  2:5207209/1‑50 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAATCAGACGATTGTATGGCCGGGat                                          <  2:2949807/50‑1 (MQ=38)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGTGat                                          <  1:1028050/50‑1 (MQ=39)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGat                                          <  1:5666522/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  2:1313409/1‑49 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         <  2:87900/50‑1 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:3132620/1‑50 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         <  2:3066037/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        >  2:3480826/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  1:3268937/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACGAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:3258563/50‑1 (MQ=255)

       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      <  2:3034338/50‑1 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:2698918/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:691813/1‑50 (MQ=255)

           gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:4390839/1‑48 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:1251099/1‑47 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:870199/49‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:2524712/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:1983518/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  2:45237/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  2:2820658/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:2148192/50‑1 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 >  2:1949019/1‑49 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 >  2:3876220/1‑49 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                <  2:5101475/50‑1 (MQ=255)

               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:2600325/1‑49 (MQ=255)

                tCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaa                              >  1:5574777/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  1:5277799/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  1:1529926/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  2:1866811/1‑49 (MQ=255)

                 ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  2:1348645/1‑49 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:5074104/1‑50 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  2:1616395/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  2:126731/49‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  2:2416746/49‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:268598/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:2944133/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  2:3920518/50‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  2:1902488/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  2:2248041/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  2:3116101/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:2931367/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  2:4152028/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACa                         <  1:3132790/50‑1 (MQ=255)

                      aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       >  1:1145352/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      >  2:5681371/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  2:2463283/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  2:5279878/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  2:5526062/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  2:5135015/50‑1 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:2712003/1‑49 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  2:2653295/1‑49 (MQ=255)

                         aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                    <  2:2054392/50‑1 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  2:1516369/1‑50 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 <  2:4712281/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:1704433/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               <  1:3470942/50‑1 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              >  2:3263312/1‑50 (MQ=255)

                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc              <  1:3934763/50‑1 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:1140005/1‑50 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:3770864/1‑49 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:5153196/1‑49 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:2501622/50‑1 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  2:4344055/50‑1 (MQ=255)

                                   cGATTGTATGCCCGGGATATTGTACAGAAAAAACAGTCTGACATACCGa           >  1:2793108/1‑49 (MQ=39)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          <  2:5131039/50‑1 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa           >  2:1081780/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  2:4588447/50‑1 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  1:5407814/50‑1 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  2:986371/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:1683480/1‑49 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCCGACATACCGaa          >  1:5100175/1‑49 (MQ=39)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa        <  2:3734110/50‑1 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa        <  2:5673961/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       <  1:4747648/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:2346217/1‑50 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg      <  1:2970952/49‑1 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:3838504/1‑50 (MQ=255)

                                        gTATGACCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  2:787673/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  2:4939194/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  2:5405031/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    <  2:2517995/50‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  2:666617/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    <  2:5510140/50‑1 (MQ=255)

                                          aTGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  2:3836476/1‑49 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  2:1521845/49‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  1:1857589/49‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  1:4046471/49‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  1:2721864/50‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  1:968947/50‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  2:1736288/50‑1 (MQ=255)

                                            gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  2:3328809/1‑49 (MQ=255)

                                            gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  1:5600509/1‑49 (MQ=255)

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`