Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)10→9	intergenic (‑141/+47)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,013	0	G	.	100.0%	98.0 / NA	21	intergenic (‑132/+56)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (8/13);  total (8/13)

TGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379964‑380057                                                  |                                             tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:4171/1‑50 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:1941674/1‑50 (MQ=255)   gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                            <  1:1073125/50‑1 (MQ=255)   gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              <  1:2191558/48‑1 (MQ=255)    agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                           >  1:1672266/1‑50 (MQ=255)      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                         >  1:1170109/1‑50 (MQ=255)          aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     <  1:1717142/50‑2 (MQ=255)            aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggct                                    >  1:1974608/1‑47 (MQ=255)               tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt                                <  1:2061630/49‑1 (MQ=255)               tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATc                               >  1:1820513/1‑50 (MQ=255)                aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATc                               <  1:68945/49‑1 (MQ=255)                aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATc                               <  1:1527942/49‑1 (MQ=255)                aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCg                              >  1:2031738/1‑50 (MQ=255)                 tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCg                              <  1:1544270/49‑1 (MQ=255)                 tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCg                              <  1:589805/49‑1 (MQ=255)                   cGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCa                           <  1:1712927/50‑1 (MQ=255)                        aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGa                       <  1:810786/49‑1 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                     >  1:2052168/1‑50 (MQ=255)                          tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg                    <  1:330800/50‑1 (MQ=255)                            tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                  <  1:287216/50‑1 (MQ=255)                              gTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgccgc                >  1:394119/1‑50 (MQ=255)                              gTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgccgc                >  1:781884/1‑50 (MQ=255)                                  gAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg            <  1:733951/50‑1 (MQ=255)                                   aaaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg           <  1:524776/50‑1 (MQ=255)                                       cAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg       <  1:1997929/50‑1 (MQ=255)                                        aaaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc      <  1:2245933/50‑1 (MQ=255)                                        aaaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc      >  1:141373/1‑50 (MQ=255)                                           gagGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg   >  1:286269/1‑50 (MQ=255)                                            agGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc  >  1:1642985/1‑50 (MQ=255)                                                  |                                             TGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379964‑380057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑