Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	160.5 / NA	35	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/15);  total (20/15)

CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765141‑765233                                                 |                                             ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                             <  1:1042444/50‑1 (MQ=255)      tttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       >  1:244455/1‑50 (MQ=39)       ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       >  1:1220133/1‑49 (MQ=38)       ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       <  1:5856/49‑1 (MQ=38)         gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    >  1:1107716/1‑50 (MQ=39)          tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    <  1:683179/49‑1 (MQ=38)          tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  1:125224/50‑1 (MQ=39)          tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  1:1024562/50‑1 (MQ=39)           gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                  >  1:677049/1‑50 (MQ=39)            cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 <  1:1737623/50‑1 (MQ=39)             ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 >  1:975140/1‑49 (MQ=38)             ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                 >  1:2169137/1‑49 (MQ=38)              tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                               >  1:234122/1‑50 (MQ=39)                tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                             <  1:1843399/50‑1 (MQ=39)                tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                             <  1:1631314/50‑1 (MQ=39)                  gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            <  1:414564‑M1/49‑1 (MQ=255)                    gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         >  1:1870609‑M1/1‑47 (MQ=255)                    gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         >  1:320593‑M1/1‑47 (MQ=255)                     atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        <  1:585450‑M1/50‑5 (MQ=255)                     atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        <  1:342528‑M1/50‑5 (MQ=255)                     atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         >  1:2121711‑M1/1‑46 (MQ=255)                       gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                      >  1:1941997‑M1/1‑44 (MQ=255)                       gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                      >  1:684157‑M1/1‑44 (MQ=255)                        atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                      <  1:268601‑M1/49‑7 (MQ=255)                           ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                   >  1:214054‑M1/1‑40 (MQ=255)                              gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc               >  1:1271645‑M1/1‑37 (MQ=255)                                   gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:1717615‑M1/1‑32 (MQ=255)                                    cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:1679374‑M1/1‑31 (MQ=255)                                       tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca      <  1:531319‑M1/50‑23 (MQ=255)                                         aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata    >  1:1494506‑M1/1‑26 (MQ=255)                                         aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat     <  1:39515‑M1/49‑24 (MQ=255)                                          gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat   >  1:1263464‑M1/1‑25 (MQ=255)                                          gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat   <  1:1583875‑M1/50‑26 (MQ=255)                                          gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat   >  1:1794058‑M1/1‑25 (MQ=255)                                           ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg  >  1:380626‑M1/1‑24 (MQ=255)                                           ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg  <  1:2192644‑M1/50‑27 (MQ=255)                                                 |                                             CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765141‑765233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑